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Odontogenic ameloblast-associated and amelotin are novel basal lamina components

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Abstract

Odontogenic ameloblast-associated (ODAM) and amelotin (AMTN) are secreted by maturation stage ameloblasts and accumulate at the interface with enamel where an atypical basal lamina (BL) is present. This study aimed at determining and quantifying the ultrastructural distribution of ODAM and AMTN at the cell–tooth interface. Ultrathin sections of enamel organs from the early to mid- and late maturation stage of amelogenesis were processed for immunogold labeling with antibodies against ODAM, AMTN or with the lectins wheat germ agglutinin, Helix pomatia agglutinin (HPA) and Ricinus communis I agglutinin. Immunolabeling showed that both ODAM and AMTN localized to the BL. Quantitative analyses indicated that at the beginning of maturation there is a concentration of ODAM on the cell side of the BL while AMTN appears more concentrated on the enamel side. In the late maturation stage, such differential distribution is no longer apparent. All three lectins are bound to the BL. Competitive incubation with native lectins did not affect the binding efficiency of ODAM; however, AMTN binding was significantly reduced after incubation with HPA. In conclusion, ODAM and AMTN are bona fide components of the BL associated with maturation stage ameloblasts and they organize into different subdomains during the early maturation stage. The data also suggest that the BL is a dynamic structure that rearranges its organization as enamel maturation advances. Finally, the abrogation of AMTN antibody labeling by HPA supports the presence of O-linked sugars in the molecule and/or its close association with other O-glycosylated molecules.

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Notes

  1. While this manuscript was under review, a paper dealing with the comparative temporospatial expression profile of murine AMTN protein during amelogenesis also reported the presence of AMTN in the basal lamina (Somogyi-Ganss et al. 2011).

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Acknowledgments

Juliana dos Santos Neves is a PhD student at the Department of Morphology, Piracicaba Dental School, State University of Campinas, SP, Brazil, and a visiting student at the Laboratory for the Study of Calcified Tissues and Biomaterials, Université de Montréal, QC, Canada. This exchange was supported by CAPES, Government of Brazil (proc. 2294-10-9). We extend our thanks to Ms Cynthia Török for technical assistance. The study was supported by the Canadian Institutes of Health Research. Pierre Moffatt is supported by the Shriners of North America.

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Authors and Affiliations

  1. Laboratory for the Study of Calcified Tissues and Biomaterials, Faculty of Dentistry, Université de Montréal, P.O. Box 6128, Station Centre-Ville, Montreal, QC, H3C 3J7, Canada

    Juliana Dos Santos Neves, Rima M. Wazen, Shingo Kuroda, Sylvia Francis Zalzal & Antonio Nanci

  2. Shriners Hospital for Children, 1529 Cedar Avenue, Montreal, QC, H3G 1A6, Canada

    Pierre Moffatt

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  1. Juliana Dos Santos Neves
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  2. Rima M. Wazen
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  3. Shingo Kuroda
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  4. Sylvia Francis Zalzal
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  5. Pierre Moffatt
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  6. Antonio Nanci
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Correspondence to Antonio Nanci.

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Dos Santos Neves, J., Wazen, R.M., Kuroda, S. et al. Odontogenic ameloblast-associated and amelotin are novel basal lamina components. Histochem Cell Biol 137, 329–338 (2012). https://doi.org/10.1007/s00418-011-0901-4

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  • DOI: https://doi.org/10.1007/s00418-011-0901-4

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