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Expression of pro-inflammatory TACE-TNF-α-amphiregulin axis in Sjögren’s syndrome salivary glands

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Abstract

The tumor-necrosis-factor-converting-enzyme (TACE)-TNF-α-Amphiregulin (AREG) axis plays an important pathogenic role in inflammatory and autoimmune disorders. However, the pathological roles of these proteins in the chronic autoimmune disease Sjögren’s syndrome (SS) remain to be elucidated. It is known that the TACE–AREG axis is clearly part of a larger cascade of signals that starts with the activation of Furin, responsible for maturation of TACE that, in turn, determines the production of active TNF-α, directly involved in the up-regulation of AREG expression. This study showed that Furin, TACE, TNF-α, and AREG proteins, detected in acinar and ductal cells of human salivary glands from SS patients, increased remarkably in comparison with biopsies of labial salivary glands from healthy controls. The changes in Furin, TACE, TNF- α, and AREG proteins’ level detected in salivary glands biopsies of SS patients could be responsible for pro-inflammatory cytokines overexpression characterizing Sjögren’s syndrome.

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Acknowledgments

This work was supported by grant (No: 20216000056) from the Italian Ministry for Universities and Research. We are grateful to M.V.C. Pragnell, B.A., for critical reading of the manuscript.

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Authors and Affiliations

  1. Department of Human Anatomy and Histology, Laboratory of Cell Biology, University of Bari Medical School, Piazza Giulio Cesare 1, 70124, Bari, Italy

    Margherita Sisto, Sabrina Lisi & Vincenzo Mitolo

  2. Department of Biological and Environmental Sciences and Technologies, University of Salento, Lecce, Italy

    Dario Domenico Lofrumento

  3. Department of Pathological Anatomy, University of Bari Medical School, Bari, Italy

    Giuseppe Ingravallo

  4. Department of Internal Medicine and Public Medicine, Section of Rheumatology, University of Bari Medical School, Bari, Italy

    Massimo D’Amore

Authors
  1. Margherita Sisto
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  2. Sabrina Lisi
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  3. Dario Domenico Lofrumento
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  4. Giuseppe Ingravallo
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  5. Vincenzo Mitolo
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  6. Massimo D’Amore
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Correspondence to Margherita Sisto.

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Margherita Sisto and Sabrina Lisi have equally contributed to this work and both are equally considered as “first author”.

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Sisto, M., Lisi, S., Lofrumento, D.D. et al. Expression of pro-inflammatory TACE-TNF-α-amphiregulin axis in Sjögren’s syndrome salivary glands. Histochem Cell Biol 134, 345–353 (2010). https://doi.org/10.1007/s00418-010-0735-5

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