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Association of the tumor necrosis factor-alpha −308 G/A polymorphism with nasal polyposis

  • Rhinology
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Abstract

Nasal polyposis (NP) is a chronic inflammatory disease in which several molecular and cellular interactions play important roles. Tumor necrosis factor-alpha (TNF-α) is a major pro-inflammatory cytokine with a key role in immune inflammatory responses in NP. Altered levels of TNF-α, which may occur due to polymorphisms in the TNF-α promoter region, may also be associated with NP susceptibility. Given these facts, we investigated the possible association of the TNF-α −308 G/A single nucleotide polymorphism (SNP) with NP. In this study, 97 consecutive adult patients with NP and 95 age- and gender-matched controls were recruited. For identification of SNP, restriction fragment length polymorphism analysis after polymerase chain reaction was carried out. The NP group had a significantly higher rate of polymorphism compared to controls (p = 0.015). Logistic regression analysis revealed that the presence of the TNF-α −308 G/A SNP is an independent risk factor for NP development (OR, 3.68; CI, 1.27–10.7; p = 0.016). The presence of a mutation failed to influence disease severity on the basis of resistance to medical and/or surgical treatment. This study suggests a possible linkage of a SNP in the TNF-α promoter with NP. These results need to be confirmed with multicentre studies for more precise interpretation and corroborative studies for investigating the influence of polymorphism on transcriptional activity.

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Acknowledgments

This study was supported by Ankara University Research Fund (Project Number 20040809181). The authors would like to thank Zeynep Biyikli and Dr. Zekeriya Alanoglu for help on statistical analysis. Study protocol of this project was approved by Ethics Committee of Ankara University Medical School.

Conflict of interest statement

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Otolaryngology Department, Elazig State Hospital, Elazig, Turkey

    Hunkar Batikhan

  2. Otorhinolaryngology, Head and Neck Surgery Department, Ankara University Medical School, Ankara, Turkey

    Mustafa Kursat Gokcan, Esen Beder & Muharrem Gerceker

  3. Pediatric Molecular Genetics Department, Ankara University Medical School, Ankara, Turkey

    Nejat Akar

  4. Biotechnology Institute, Ankara University Medical School, Ankara, Turkey

    Aysenur Ozturk

  5. Koza Sokak Ikizler Sitesi 72B/39, G.O.P., 06700, Ankara, Turkey

    Mustafa Kursat Gokcan

Authors
  1. Hunkar Batikhan
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  2. Mustafa Kursat Gokcan
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  3. Esen Beder
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  4. Nejat Akar
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  5. Aysenur Ozturk
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  6. Muharrem Gerceker
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Corresponding author

Correspondence to Mustafa Kursat Gokcan.

Additional information

Presented as a poster at the 8th International Otorhinolaryngology Head and Neck Surgery Meeting, Ankara, Turkey, 15–17 May 2008.

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Batikhan, H., Gokcan, M.K., Beder, E. et al. Association of the tumor necrosis factor-alpha −308 G/A polymorphism with nasal polyposis. Eur Arch Otorhinolaryngol 267, 903–908 (2010). https://doi.org/10.1007/s00405-009-1167-5

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  • DOI: https://doi.org/10.1007/s00405-009-1167-5

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