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Effects of superovulation, in vitro fertilization, and oocyte in vitro maturation on imprinted gene Grb10 in mouse blastocysts

  • Gynecologic Endocrinology and Reproductive Medicine
  • Published:
Archives of Gynecology and Obstetrics Aims and scope Submit manuscript

Abstract

Purpose

Grb10 is a key imprinted gene that is suspected to have a role in the adverse outcomes of assisted reproductive technology (ART), but little is known about the effects of ART on it. Primary ART techniques, including superovulation, in vitro fertilization (IVF), and oocyte in vitro maturation (IVM), were analyzed in this study of the effects of ART on embryo quality and Grb10.

Methods

Embryo development rates were determined. Blastocyst cell number and global methylation were analyzed at the single-embryo level, together with Grb10 methylation and mRNA expression of the imprinted genes.

Results

Lower blastocyst cell number, higher genome and Grb10 CGI1 methylation, and variable mRNA expression were observed in the ART groups compared with the control group. Whether fertilization was in vivo or in vitro, the changes in the genome and Grb10 CGI1 methylation level and Grb10 and H19 expression were similar in the groups with superovulation and more significant than the IVM group.

Conclusions

These results suggest that superovulation had a greater impact than IVF or IVM on the genome and Grb10 DNA methylation level, and Grb10 and H19 expression.

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Funding

This work was supported by the National Natural Science Foundation of China (Grant number: 31071311), and the Project of Fuzhou Science and Technology Plan of Fuzhou (Grant number: 2014-S-146).

Author information

Authors and Affiliations

  1. The School of Basic Medical Science, Fujian Medical University, No. 88 Jiaotong Road, Fuzhou, 350003, Fujian, People’s Republic of China

    Xuan Chen, Yanfang Huang, Hongfeng Huang, Yingying Guan, Ming Li, Xiaohong Jiang, Miao Yu & Xiaoyu Yang

  2. The First Affiliated Hospital of Fujian Medical University, Fuzhou, People’s Republic of China

    Yanfang Huang

  3. Fuzhou Maternity and Child Health Care Hospital, Fuzhou, People’s Republic of China

    Xiaoyu Yang

Authors
  1. Xuan Chen
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  2. Yanfang Huang
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  3. Hongfeng Huang
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  4. Yingying Guan
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  5. Ming Li
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  6. Xiaohong Jiang
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  7. Miao Yu
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  8. Xiaoyu Yang
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Contributions

XC: protocol and project development, data management, data analysis, manuscript writing and manuscript editing. YH: protocol and project development, and data management. HH: protocol and project development, and data collection. YG: protocol and project development, and data collection. ML: data collection, protocol and project development. XJ: protocol and project development, and data management. MY: protocol and project development, and data management. XY: protocol and project development, data analysis, and manuscript editing.

Corresponding author

Correspondence to Xiaoyu Yang.

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Chen, X., Huang, Y., Huang, H. et al. Effects of superovulation, in vitro fertilization, and oocyte in vitro maturation on imprinted gene Grb10 in mouse blastocysts. Arch Gynecol Obstet 298, 1219–1227 (2018). https://doi.org/10.1007/s00404-018-4905-3

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  • DOI: https://doi.org/10.1007/s00404-018-4905-3

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