Abstract
Tissue engineering and regenerative medicine research is being aggressively pursued in attempts to develop biological substitutes to replace lost tissue or organs. Remarkable degrees of success have been achieved in the generation of a variety of tissues and organs as a result of concerted contributions by multidisciplinary groups in the field of biotechnology. Engineering of an organ is a complex process which is initiated by appropriate sourcing of cells and their controlled proliferation to achieve critical numbers for seeding on biodegradable scaffolds in order to create cell-scaffold constructs, which are thereafter maintained in bioreactors to generate tissues identical to those required for replacement. Extensive efforts in understanding the characteristics of cells and their interaction with specifically tailored scaffolds holds the key to their attachment, controlled proliferation and differentiation, intercommunication, and organization to form tissues. The demand for tissue-engineered organs is enormous and this technology holds the promise to supply customized organs to overcome the severe shortages that are currently faced by the pediatric patient, especially due to organ-size mismatch. The contemporary state of tissue-engineering technology presented in this review summarizes the advances in the various areas of regenerative medicine and addresses issues that are associated with its future implementation in the pediatric surgical patient.
Similar content being viewed by others
References
Langer R, Vacanti JP (1993) Tissue engineering. Science 260:920–926
Lysaght MJ, O’Loughlin JA (2000) Demographic scope and economic magnitude of contemporary organ replacement therapies. ASAIO J 46:515–521
Eurotransplant International Foundation Annual Report 2008. In: Oosterlee A, Rahmel A (eds) Eurotransplant International Foundation, Leiden, The Netherlands. ISBN-13: 978-90-71658-28-0
2008 Annual Report of the U.S. Organ Procurement and Transplantation Network and the Scientific Registry of Transplant Recipients: Transplant Data 1998–2007. U.S. Department of Health and Human Services, Health Resources and Services Administration, Healthcare Systems Bureau, Division of Transplantation, Rockville, MD
Giovanelli M, Gupte GL, McKiernan P et al (2009) Impact of change in the United Kingdom pediatric donor organ allocation policy for intestinal transplantation. Transplantation 87:1695–1699
Tiao GM, Alonso MH, Ryckman FC (2006) Pediatric liver transplantation. Semin Pediatr Surg 15:218–227
Harada KM, Mandia-Sampaio EL, de Sandes-Freitas TV et al (2009) Risk factors associated with graft loss and patient survival after kidney transplantation. Transplant Proc 41:3667–3670
Reding R (2005) Long-term complications of immunosuppression in pediatric liver recipients. Acta Gastroenterol Belg 68:453–456
Magee JC, Krishnan SM, Benfield MR (2008) Pediatric transplantation in the United States, 1997–2006. Am J Transplant 8:935–945
Golomb J, Klutke CG, Lewin KJ et al (1989) Bladder neoplasms associated with augmentation cystoplasty: report of 2 cases and literature review. J Urol 142:377–380
Castagna MT, Mintz GS, Ohlmann P et al (2005) Incidence, location, magnitude, and clinical correlates of saphenous vein graft calcification: an intravascular ultrasound and angiographic study. Circulation 111:1148–1152
Arul GS, Parikh D (2008) Oesophageal replacement in children. Ann R Coll Surg Engl 90:7–12
Jaffe R, Strauss BH (2007) Late and very late thrombosis of drug-eluting stents: evolving concepts and perspectives. J Am Coll Cardiol 50:119–127
Guyen O, Lewallen DG, Cabanela ME (2008) Modes of failure of osteonics constrained tripolar implants: a retrospective analysis of forty-three failed implants. J Bone Joint Surg Am 90:1553–1560
Yukata K, Doi K, Hattori Y et al (2009) Early breakage of a titanium volar locking plate for fixation of a distal radius fracture: case report. J Hand Surg Am 34:907–909
Schildhauer TA, Robie B, Muhr G et al (2006) Bacterial adherence to tantalum versus commonly used orthopedic metallic implant materials. J Orthop Trauma 20:476–484
Jeandidier N, Riveline JP, Tubiana-Rufi N et al (2008) Treatment of diabetes mellitus using an external insulin pump in clinical practice. Diabetes Metab 34:425–438
Piaggesi A (2004) Research development in the pathogenesis of neuropathic diabetic foot ulceration. Curr Diab Rep 4:419–423
Senker J, Enzing C, Joly PB et al (2000) European exploitation of biotechnology-do government policies help? A recent survey of public spending on biotechnology in Europe suggests that money alone cannot stimulate growth of the sector. Nat Biotechnol 18:605–608
Tabata Y (2009) Biomaterial technology for tissue engineering applications. J R Soc Interface 6(Suppl 3):S311–S324
Williams DF (2009) On the nature of biomaterials. Biomaterials 30:5897–5909
Burdick JA, Vunjak-Novakovic G. Engineered microenvironments for controlled stem cell differentiation. Tissue Eng Part A 15:205–219
Carrel A, Lindbergh C (1938) The culture of organs. Paul B. Hoeber Inc., Harper Brothers, New York
Bianco P, Robey PG (2001) Stem cells in tissue engineering. Nature 414:118–121
Vats A, Bielby RC, Tolley NS et al (2005) Stem cells. Lancet 366:592–602
Thomson JA et al (1998) Embryonic stem cell lines derived from human blastocysts. Science 282:1145–1147
Solter D, Gearhart J (1999) Putting stem cells to work. Science 283:1468–1470
Vogel G (1999) Harnessing the power of stem cells. Science 283:1432–1434
Amit M, Carpenter MK, Inokuma MS et al (2000) Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged periods of culture. Dev Biol 227:271–278
Odorico JS, Kaufman DS, Thomson JA (2001) Multilineage differentiation from human embryonic stem cell lines. Stem Cells 19:193–204
Cowan CA, Klimanskaya I, McMahon J et al (2004) Derivation of embryonic stem-cell lines from human blastocysts. N Engl J Med 350:1353–1356
Schuldiner M, Itskovitz-Eldor J, Benvenisty N (2003) Selective ablation of human embryonic stem cells expressing a “suicide” gene. Stem Cells 21:257–265
Drukker M, Katz G, Urbach A et al (2002) Characterization of the expression of MHC proteins in human embryonic stem cells. Proc Natl Acad Sci USA 99:9864–9869
Lysaght MJ (2003) Immunosuppression, immunoisolation and cell therapy. Mol Ther 7:432
Hall VJ, Stojkovic P, Stojkovic M (2006) Using therapeutic cloning to fight human disease: a conundrum or reality? Stem Cells 24:1628–1637
Colman A, Kind A (2000) Therapeutic cloning: concepts and practicalities. Trends Biotechnol 18:192–196
Priddle H, Jones DR, Burridge PW et al (2006) Hematopoiesis from human embryonic stem cells: overcoming the immune barrier in stem cell therapies. Stem Cells 24:815–824
Raikwar SP, Mueller T, Zavazava N (2006) Strategies for developing therapeutic application of human embryonic stem cells. Physiology (Bethesda) 21:19–28
Tian X, Kaufman DS (2005) Hematopoietic development of human embryonic stem cells in culture. Methods Mol Med 105:425–436
Trounson A (2006) The production and directed differentiation of human embryonic stem cells. Endocr Rev 27:208–219
Leker RR, McKay RD (2004) Using endogenous neural stem cells to enhance recovery from ischemic brain injury. Curr Neurovasc Res 1:421–427
Beltrami AP, Barlucchi L, Torella D et al (2003) Adult cardiac stem cells are multipotent and support myocardial regeneration. Cell 114:763–776
Kuroda R, Usas A, Kubo S et al (2006) Cartilage repair using bone morphogenetic protein 4 and muscle-derived stem cells. Arthritis Rheum 54:433–442
Walkup MH, Gerber DA (2006) Hepatic stem cells: in search of. Stem Cells 24:1833–1840
Zalzman M, Anker-Kitai L, Efrat S (2005) Differentiation of human liver-derived, insulin-producing cells toward the beta-cell phenotype. Diabetes 54:2568–2575
Raghunath J, Salacinski HJ, Sales KM et al (2005) Advancing cartilage tissue engineering: the application of stem cell technology. Curr Opin Biotechnol 16:503–509
Riha GM, Lin PH, Lumsden AB, Yao Q (2005) Review: application of stem cells for vascular tissue engineering. Tissue Eng 11:1535–1552
Risbud MV, Shapiro IM (2005) Stem cells in craniofacial and dental tissue engineering. Orthod Craniofac Res 8:54–59
Bruder SP, Fink DJ, Caplan AI (1994) Mesenchymal stem cells in bone development, bone repair, and skeletal regeneration therapy. J Cell Biochem 56:283–294
Gimble J, Guilak F (2003) Adipose-derived adult stem cells: isolation, characterization, and differentiation potential. Cytotherapy 5:362–369
De Coppi P, Bartsch G, Siddiqui MM et al (2007) Isolation of amniotic stem cell lines with potential for therapy. Nat Biotechnol 25:100–106
Miki T, Lehmann T, Cai H et al (2005) Stem cell characteristics of amniotic epithelial cells. Stem Cells 23:1549–1559
Saxena AK (2005) Tissue engineering: present concepts and strategies. J Indian Assoc Pediatr Surg 10:14–19
Langer R, Tirrell DA (2004) Designing materials for biology and medicine. Nature 428:487–492
Boccaccini AR, Blaker JJ (2005) Bioactive composite materials for tissue engineering scaffolds. Expert Rev Med Devices 2:303–317
Behonick DJ, Werb Z (2003) A bit of give and take: the relationship between the extracellular matrix and the developing chondrocyte. Mech Dev 120:1327–1336
Ma Z, He W, Yong T et al (2005) Grafting of gelatin on electrospun poly(caprolactone) nanofibers to improve endothelial cell spreading and proliferation and to control cell orientation. Tissue Eng 11:1149–1158
Rho KS, Jeong L, Lee G et al (2006) Electrospinning of collagen nanofibers: effects on the behavior of normal human keratinocytes and early-stage wound healing. Biomaterials 27:1452–1461
Ayutsede J, Gandhi M, Sukigara S et al (2006) Carbon nanotube-reinforced Bombyx morisilk nanofibers by the electrospinning process. Biomacromolecules 7:208–224
Stankus JJ, Guan J, Fujimoto K et al (2006) Microintegrating smooth muscle cells into a biodegradable, elastomeric fiber matrix. Biomaterials 27:735–744
Lee KY, Mooney DJ (2001) Hydrogels for tissue engineering. Chem Rev 101:1869–1880
Nguyen KT, West JL (2002) Photopolymerizable hydrogels for tissue engineering applications. Biomaterials 23:4307–4314
Lutolf MP, Hubbell JA (2005) Synthetic biomaterials as instructive extracellular microenvironments for morphogenesis in tissue engineering. Nat Biotechnol 23:47–55
Tibbitt MW, Anseth KS (2009) Hydrogels as extracellular matrix mimics for 3D cell culture. Biotechnol Bioeng 103:655–663
Grayson WL, Zhao F, Izadpanah R et al (2006) Effects of hypoxia on human mesenchymal stem cell expansion and plasticity in 3D constructs. J Cell Physiol 207:331–339
Niklason LE, Gao J, Abbott WM et al (1999) Functional arteries grown in vitro. Science 284:489–493
Barron V, Lyons E, Stenson-Cox C et al (2003) Bioreactors for cardiovascular cell and tissue growth: a review. Ann Biomed Eng 31:1017–1030
Eschenhagen T, Fink C, Remmers U et al (1997) Three-dimensional reconstitution of embryonic cardiomyocytes in a collagen matrix: a new heart model system. FASEB J 11:683–694
Carrier RL, Papadaki M, Rupnick M et al (1999) Cardiac tissue engineering: cell seeding, cultivation parameters and tissue construct characterization. Biotechnol Bioeng 64:580–589
Zimmermann WH, Melnychenko I, Wasmeier G et al (2006) Engineered heart tissue grafts improve systolic and diastolic function in infracted rat hearts. Nat Med 12:452–458
Guo XM, Zhao YS, Chang HX et al (2006) Creation of engineered cardiac tissue in vitro from mouse embryonic stem cells. Circulation 113:2229–2237
Shimizu T, Yamato M, Isoi Y et al (2002) Fabrication of pulsatile cardiac tissue grafts using a novel 3-dimensional cell sheet manipulation technique and temperature-responsive cell culture surfaces. Cir Res 90:e40–e48
Guo Y, Zhang XZ, Wei Y et al (2009) Culturing of ventricle cells at high density and construction of engineered cardiac cell sheets without scaffold. Int Heart J 50:653–662
Shinoka T, Ma PX, Shum-Tim D et al (1996) Tissue-engineered heart valves. Autologous valve leaflet replacement study in a lamb model. Circulation 94(Suppl 9):II164–II168
Schnell AM, Hoerstrup SP, Zund G et al (2001) Optimal cell source for cardiovascular tissue engineering: venous vs. aortic human myofibroblasts. Thorac Cardiovasc Surg 49:221–225
Sutherland FWH, Perry TE, Nasseri BA et al (2002) Advances in the mechanisms of cell delivery to cardiovascular scaffolds: comparison of two rotating cell culture systems. ASAIO J 48:346–349
Engelmayr GC, Hildebrand DK, Sutherland FW et al (2003) A novel bioreactor for the dynamic flexural stimulation of tissue engineered heart-valve biomaterials. Biomaterials 24:2523–2532
Dohmen PM, Lembcke A, Hotz H et al (2002) Ross operation with a tissue-engineered heart valve. Ann Thorac Surg 74:1438–1442
Dohmen PM, Lembcke A, Holinski S et al (2007) Mid-term clinical results using a tissue-engineered pulmonary valve to reconstruct the right ventricular outflow tract during the Ross procedure. Ann Thorac Surg 84:729–736
Shinoka T, Breuer C (2008) Tissue-engineered blood vessels in pediatric cardiac surgery. Yale J Biol Med 81:161–166
Narushima M, Kobayashi N, Okitsu T et al (2005) A human beta-cell line for transplantation therapy to control type 1 diabetes. Nat Biotechnol 23:1274–1282
Yanagita M, Nakayama K, Takeuchi T (1992) Processing of mutated proinsulin with tetrabasic cleavage sites to bioactive insulin in the nonendocrine cell line, COS-7. FEBS Lett 311:55–59
Bonner-Weir S, Sharma A (2002) Pancreatic stem cells. J Pathol 197:519–526
Jun HS, Yoon JW (2005) Approaches for the cure of type 1 diabetes by cellular and gene therapy. Curr Gene Ther 5:249–262
Mikos A, Papadaki M, Kouvroukoglou S et al (1994) Mini-review: islet transplantation to create a bioartificial pancreas. Biotechnol Bioeng 43:673–677
Soon-Shiong P, Heintz RE, Merideth N et al (1994) Insulin independence in a type 1 diabetic patient after encapsulated islet transplantation. Lancet 343:950–951
Calafiore R, Basta G, Luca G et al (2006) Microencapsulated pancreatic islet allografts into nonimmunosuppressed patients with type 1 diabetes: first two cases. Diabetes Care 29:137–138
Tuch BE, Keogh GW, Williams LJ et al (2009) Safety and viability of microencapsulated human islets transplanted into diabetic humans. Diabetes Care 32:1887–1889
Takimoto Y, Okumura N, Nakamura T et al (1993) Long-term follow-up of the experimental replacement of the esophagus with a collagen–silicone composite tube. ASAIO J 39:M736–M739
Yamamoto Y, Nakamura T, Shimizu Y et al (1999) Intrathoracic esophageal replacement in the dog with the use of an artificial esophagus composed of a collagen sponge with a double-layered silicone tube. J Thorac Cardiovasc Surg 118:276–286
Yamamoto Y, Nakamura T, Shimizu Y et al (2000) Intrathoracic esophageal replacement with a collagen sponge–silicone double-layer tube: evaluation of omental-pedicle wrapping and prolonged placement of an inner stent. ASAIO J 46:734–739
Hori Y, Nakamura T, Kimura D et al (2003) Effect of basic fibroblast growth factor on vascularization in esophagus tissue engineering. Int J Artif Organs 26:241–244
Sato M, Ando N, Ozawa S et al (1994) An artificial esophagus consisting of cultured human esophageal epithelial cells, polyglycolic acid mesh, and collagen. ASAIO J 40:M389–M392
Hayashi K, Ando N, Ozawa S et al (2004) A neo-esophagus reconstructed by cultured human esophageal epithelial cells, smooth muscle cells, fibroblasts, and collagen. ASAIO J 50:261–266
Badylak S, Meurling S, Chen M et al (2000) Resorbable bioscaffold for esophageal repair in a dog model. J Pediatr Surg 35:1097–1103
Badylak SF, Vorp DA, Spievack AR et al (2005) Esophageal reconstruction with ECM and muscle tissue in a dog model. J Surg Res 128:87–97
Doede T, Bondartschuk M, Joerck C et al (2009) Unsuccessful alloplastic esophageal replacement with porcine small intestinal submucosa. Artif Organs 33:328–333
Nakase Y, Nakamura T, Kin S et al (2008) Intrathoracic esophageal replacement by in situ tissue-engineered esophagus. J Thorac Cardiovasc Surg 136:850–859
Grikscheit T, Ochoa ER, Srinivasan A et al (2003) Tissue-engineered esophagus: experimental substitution by onlay patch or interposition. J Thorac Cardiovasc Surg 126:537–544
Saxena AK, Ainoedhofer H, Höllwarth ME (2009) Esophagus tissue engineering: in vitro generation of esophageal epithelial cell sheets and viability on scaffold. J Pediatr Surg 44:896–901
Saxena AK, Kofler K, Ainödhofer H et al (2009) Esophagus tissue engineering: hybrid approach with esophageal epithelium and unidirectional smooth muscle tissue component generation in vitro. J Gastrointest Surg 13:1037–1043
Soltysiak P, Saxena AK (2009) Micro-computed tomography for implantation site imaging during in situ oesophagus tissue engineering in a live small animal model. J Tissue Eng Regen Med 3:573–576
Saxena AK, Ainoedhofer H, Höllwarth ME (2010) Culture of ovine esophageal epithelial cells and in vitro esophagus tissue engineering. Tissue Eng Part C Methods 16:109–114
Kofler K, Ainoedhofer H, Höllwarth ME et al (2010) Fluorescence-activated cell sorting of PCK-26 antigen-positive cells enables selection of ovine esophageal epithelial cells with improved viability on scaffolds for esophagus tissue engineering. Pediatr Surg Int 26:97–104
Saxena AK, Soltysiak P, Ainoedhofer H (2009) Esophagus tissue engineering: In situ generation of vascularized esophageal conduits using the ovine model. Abstracts of the 41st Annual Meeting of the Canadian Association of Pediatric Surgeons, Oct 1–4, Halifax, Nova Scotia, Canada
Vacanti JP, Morse MA, Saltzman WM et al (1998) Selective cell transplantation using bioabsorbable artificial polymers as matrices. J Pediatr Surg 23:3–9
Patel HR, Tait IS, Evans GS et al (1996) Influence of cell interactions in a novel model of postnatal mucosal regeneration. Gut 38:679–686
Evans GS, Flint N, Somers AS et al (1992) The development of a method for the preparation of rat intestinal epithelial cell primary cultures. J Cell Sci 101:219–231
Tait IS, Flint N, Campbell FC et al (1994) Generation of neomucosa in vivo by transplantation of dissociated rat postnatal small-intestinal epithelium. Differentiation 56:91–100
Tait IS, Evans GS, Flint N et al (1994) Colonic mucosal replacement by syngeneic small intestinal stem cell transplantation. Am J Surg 167:67–72
Choi RS, Riegler M, Pothoulakis C et al (1998) Studies of brush border enzymes, basement membrane components, and electrophysiology of tissue-engineered neointestine. J Pediatr Surg 33:991–996
Kim SS, Kaihara S, Benvenuto MS et al (1999) Effects of anastomosis of tissue engineered neointestine to native small bowel. J Surg Res 87:6–13
Grikscheit TC, Siddique A, Ochoa ER et al (2004) Tissue-engineered small intestine improves recovery after massive small bowel resection. Ann Surg 240:748–754
Lloyd DA, Ansari TI, Gundabolu P et al (2006) A pilot study investigating a novel subcutaneously implanted precellularized scaffold for tissue engineering of intestinal mucosa. Eur Cell Mater 11:27–33
Sala FG, Kunisaki SM, Ochoa ER et al (2009) Tissue-engineered small intestine and stomach form from autologous tissue in a preclinical large animal model. J Surg Res 156:205–212
Nony PA, Schnellmann RG (2003) Mechanisms of renal cell repair and regeneration after acute renal failure. J Pharmacol Exp Ther 304:905–912
Al-Awqati Q, Oliver JA (2002) Stem cells in the kidney. Kidney Int 61:387–395
Oliver JA, Maarouf O, Cheema FH et al (2004) The renal papilla is a niche for adult kidney stem cells. J Clin Invest 114:795–804
Duffield JS, Park KM, Hsiao LL et al (2005) Restoration of tubular epithelial cells during repair of the postischemic kidney occurs independently of bone marrow-derived stem cells. J Clin Invest 115:1743–1755
Lin F, Moran A, Igarashi P (2005) Intrarenal cells, not bone marrow–derived cells, are the major source for regeneration in postischemic kidney. J Clin Invest 115:1756–1764
Brodie JC, Humes HD (2005) Stem cell approaches for the treatment of renal failure. Pharmacol Rev 57:299–313
Steenhard BM, Isom KS, Cazcarro P et al (2005) Integration of embryonic stem cells in metanephric kidney organ culture. J Am Soc Nephrol 16:1623–1631
Wang PC, Takezawa T (2005) Reconstruction of renal glomerular tissue using collagen vitrigel scaffold. J Biosci Bioeng 99:529–540
Joraku A, Stern KA, Atala A et al (2009) In vitro generation of three-dimensional renal structures. Methods 47:129–133
Roessger A, Denk L, Minuth WW (2009) Potential of stem/progenitor cell cultures within polyester fleeces to regenerate renal tubules. Biomaterials 30:3723–3732
Kropp BP, Cheng EY, Lin HK et al (2004) Reliable and reproducible bladder regeneration using unseeded distal small intestinal ubmucosa. J Urol 172:1710–1713
Yoo JJ, Meng J, Oberpenning F et al (1998) Bladder augmentation using allogenic bladder submucosa seeded with cells. Urology 51:221–225
Probst M, Dahiya R, Carrier S et al (1997) Reproduction of functional smooth muscle tissue and partial bladder replacement. Br J Urol 79:505–515
Portis AJ, Elbahnasy AM, Shalhav AL et al (2000) Laparoscopic augmentation cystoplasty with different biodegradable grafts in an animal model. J Urol 164:1405–1411
Landman J, Olweny E, Sundaram CP et al (2004) Laparoscopic mid-sagittal hemicystectomy and bladder reconstruction with small intestinal submucosa and reimplantation of ureter into small intestinal submucosa: 1-year follow-up. J Urol 171:2450–2455
Oberpenning FO, Meng J, Yoo J et al (1999) De novo reconstitution of a functional urinary bladder by tissue engineering. Nat Biotechnol 17:149–155
Atala A, Bauer SB, Soker S et al (2006) Tissue-engineered autologous bladders for patients needing cystoplasty. Lancet 367:1241–1246
Soler R, Fullhase C, Atala A et al (2009) Regenerative medicine strategies for treatment of neurogenic bladder. Therapy 6:177–184
Guillouzo A (1998) Liver cell models in in vitro toxicology. Environ Health Perspect 106:511–532
Mitaka T (1998) The current status of primary hepatocyte culture. Int J Exp Pathol 79:393–409
Ranucci CS, Kumar A, Batra SP et al (2000) Control of hepatocyte function on collagen foams: sizing matrix pores toward selective induction of 2D and 3D cellular morphogenesis. Biomaterials 21:783–793
Seo SJ, Choi YJ, Akaike T et al (2006) Alginate/galactosylated chitosan/heparin scaffold as a new synthetic extracellular matrix for hepatocytes. Tissue Eng 12:33–44
Tan W, Desai TA (2003) Microfluidic patterning of cells in extracellular matrix biopolymers: effects of channel size, cell type, and matrix composition on pattern integrity. Tissue Eng 9:255–267
Wang X, Yan Y, Pan Y et al (2006) Generation of three-dimensional hepatocyte/gelatin structures with rapid prototyping system. Tissue Eng 12:83–90
Kaihara S, Borenstein J, Koka R et al (2000) Silicon micromachining to tissue engineer branched vascular channels for liver fabrication. Tissue Eng 6:105–117
Allen JW, Khetani SR, Bhatia SN (2005) In vitro zonation and toxicity in a hepatocyte bioreactor. Toxicol Sci 84:110–119
Gebhardt R, Hengstler JG, Muller D et al (2003) New hepatocyte in vitro systems for drug metabolism: metabolic capacity and recommendations for application in basic research and drug development, standard operation procedures. Drug Metab Rev 35:145–213
Hong KU, Reynolds SD, Giangreco A et al (2001) Clara cell secretory protein-expressing cells of the airway neuroepithelial body microenvironment include a label-retaining subset and are critical for epithelial renewal after progenitor cell depletion. Am J Respir Cell Mol Biol 24:671–681
Kim CF, Jackson EL, Woolfenden AE (2005) Identification of bronchioalveolar stem cells in normal lung and lung cancer. Cell 121:823–835
Coraux C, Nawrocki-Raby B, Hinnrasky J et al (2005) Embryonic stem cells generate airway epithelial tissue. Am J Respir Cell Mol Biol 32:87–92
Van Vranken BE, Romanska HM, Polak JM (2005) Coculture of embryonic stem cells with pulmonary mesenchyme: a microenvironment that promotes differentiation of pulmonary epithelium. Tissue Eng 11:1177–1187
Saxena AK, Marler J, Benvenuto M (1999) Skeletal muscle tissue engineering using isolated myoblasts on synthetic biodegradable polymers: preliminary studies. Tissue Eng 5:525–532
Saxena AK, Willital GH, Vacanti JP (2001) Vascularized three-dimensional skeletal muscle tissue-engineering. Biomed Mater Eng 11:275–281
Tsang VL, Bhatia SN (2004) Three-dimensional tissue fabrication. Adv Drug Deliv Rev 56:1635–1647
Costa KD, Lee EJ, Holmes JW (2003) Creating alignment and anisotropy in engineered heart tissue: Role of boundary conditions in a model three-dimensional culture system. Tissue Eng 9:567–577
Girton TS, Barocas VH, Tranquillo RT (2002) Confined compression of a tissue-equivalent: collagen fibril and cell alignment in response to anisotropic strain. J Biomech Eng 124:568–575
Taylor NA, Wilkinson JG (1986) Exercise-induced skeletal muscle growth. Hypertrophy or hyperplasia? Sports Med 3:190–200
Vandenburgh HH, Karlisch P (1989) Longitudinal growth of skeletal myotubes in vitro in a new horizontal mechanical cell stimulator. In Vitro Cell Dev Biol 25:607–616
Cheema U, Yang SY, Mudera V (2003) 3-D in vitro model of early skeletal muscle development. Cell Motil Cytoskeleton 54:226–236
Tatsumi R, Sheehan SM, Iwasaki H (2001) Mechanical stretch induces activation of skeletal muscle satellite cells in vitro. Exp Cell Res 267:107–114
Darr KC, Schultz E (1987) Exercise-induced satellite cell activation in growing and mature skeletal muscle. J Appl Physiol 63:1816–1821
Kook SH, Lee HJ, Chung WT et al (2008) Cyclic mechanical stretch stimulates the proliferation of C2C12 myoblasts and inhibits their differentiation via prolonged activation of p38 MAPK. Mol Cells 25(4):479–486
Otis JS, Burkholder TJ, Pavlath GK (2005) Stretch-induced myoblast proliferation is dependent on the COX2 pathway. Exp Cell Res 310:417–425
Fujita H, Nedachi T, Kanzaki M (2007) Accelerated de novo sarcomere assembly by electric pulse stimulation in C2C12 myotubes. Exp Cell Res 313:1853–1865
De Deyne PG (2000) Formation of sarcomeres in developing myotubes: Role of mechanical stretch and contractile activation. Am J Physiol Cell Physiol 279:C1801–C1811
Larkin LM, Van der Meulen JH, Dennis RG (2006) Functional evaluation of nerve-skeletal muscle constructs engineered in vitro. In Vitro Cell Dev Biol Anim 42:75–82
Dhawan V, Lytle IF, Dow DE (2007) Neurotization improves contractile forces of tissue-engineered skeletal muscle. Tissue Eng 13:2813–2821
Acknowledgments
Research funds from the European Union within the 6th Framework Program (EuroSTEC; LSHC-CT-2006-037409). Authors thank Symatese Biomateriaux, France and Matricel GmbH, Germany for scaffold images. The contributions of Prof. Michael E. Höllwarth, Mag. Kristina Kofler, Mrs. Anna Kuess, Mr. Herwig Ainoedhofer, Mr. Richard Ackbar, Dr. Piotr Soltysiak, Dr. Hinrich Baumgart, Dr. Christian Komann, Dr. Iris Wiederstein and Dr. Gerd Leitinger (Medical University of Graz, Austria) are gratefully appreciated.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Saxena, A.K. Tissue engineering and regenerative medicine research perspectives for pediatric surgery. Pediatr Surg Int 26, 557–573 (2010). https://doi.org/10.1007/s00383-010-2591-8
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00383-010-2591-8