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MicroRNA-19b-1 reverses ischaemia-induced heart failure by inhibiting cardiomyocyte apoptosis and targeting Bcl2 l11/BIM

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Abstract

Ischaemia induces cardiac apoptosis and leads to a loss of cardiac function and heart failure after myocardial infarction. MicroRNA-19b-1 (miR-19b-1), a key member of the miR-17/92 cluster, plays crucial roles in inhibiting apoptosis. However, the role of miR-19b-1 in ischaemia-induced heart failure remains unknown. In this study, ischaemia resulted in cardiac apoptosis and the suppression of miR-19b-1 expression, whereas miR-19b-1 overexpression inhibited ischaemia-induced cardiac apoptosis in vivo and in vitro. Moreover, miR-19b-1 not only attenuated the infarct size but also ameliorated heart failure after myocardial infarction, including the changes in the left ventricular ejection fraction and volume load. Mechanically, miR-19-1 targeted and downregulated the mRNA and protein expression of Bcl2l11/BIM, a pro-apoptotic gene of the Bcl-2 family. Together, these results revealed an essential role of miR-19b-1 in ischaemia-induced heart failure.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (81670266 and 81770384), the Science and Technology Commission of Shanghai Municipality (17140902500) and Joint Funds for the innovation of science and Technology, Fujian province (2017Y9007).

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Author notes

  1. Wenbo Yang, Yanxin Han and Chendie Yang contributed equally to this work.

Authors and Affiliations

  1. Department of Cardiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, No 197 Ruijin 2nd Road, Shanghai, 200025, People’s Republic of China

    Wenbo Yang, Yanxin Han, Chendie Yang, Yanjia Chen, Weilin Zhao, Xiuxiu Su, Ke Yang & Wei Jin

  2. Institute of Cardiovascular Disease, Shanghai Jiao Tong University School of Medicine, Shanghai, People’s Republic of China

    Ke Yang

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  1. Wenbo Yang
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Yang, W., Han, Y., Yang, C. et al. MicroRNA-19b-1 reverses ischaemia-induced heart failure by inhibiting cardiomyocyte apoptosis and targeting Bcl2 l11/BIM. Heart Vessels 34, 1221–1229 (2019). https://doi.org/10.1007/s00380-018-01336-3

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