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Intra-articular injection of hyaluronate and indomethacin in rabbits with antigen-induced arthritis

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Abstract

Combined effects of hyaluronate and indomethacin in the treatment of rabbits with antigen-induced arthritis (AIA) were evaluated by assessing joint swelling, C-reactive protein (CRP) and prostaglandin E2 (PGE2) levels with periodic intra-articular (ia) injections of hyaluronate alone (HA group) and with either a low or high concentration of indomethacin (LI-HA or HI-HA group). End-point analyses included matrix metalloproteinases-3 (MMP-3) activity and macroscopic and histological joint examinations. Results demonstrated that treatment in LI-HA and HI-HA groups resulted in statistically significant suppression of CRP, PGE2, and MMP-3 in comparison with those of HA group. Inhibition of serum CRP was only observed in LI-HA group. The order of serum MMP-3 inhibition was LI-HA>HI-HA>HA. Based on macroscopic and histological analyses of pannus formation, hyperplasia, inflammation, joint leakage and erosion, and loss of proteoglycan, the only statistically significant improvement was shown in LI-HA group compared to HA group and HI-HA group compared to control group.

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Acknowledgments

This study was sponsored by Min-Sheng Healthcare (93MSH-TMU-17). The authors would like to express sincere thank Mr. Wen-Chung Lee for her assistance with the histological staining.

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Authors and Affiliations

  1. College of Pharmacy, Taipei Medical University, Taipei, Taiwan, ROC

    Yow-Jen Lo & Ming-Thau Sheu

  2. Department of Orthopedic Surgery, Min-Sheng Healthcare, Taoyuan, Taiwan, ROC

    Wen-Chi Tsai

  3. Department of Pathology, Taipei Medical University, Taipei, Taiwan, ROC

    Yun-Ho Lin

  4. Department of Orthopedics and Traumatology, Taipei Medical University Hospital, Taipei Medical University, Taipei, Taiwan, ROC

    Jau-Le Li & Ming-Shium Hsieh

  5. School of Medical Technology, Taipei Medical University, 250 Wu-Hsing Street, Taipei, 110, Taiwan, ROC

    Yu-Chih Liang & Chien-Ho Chen

  6. Department of Internal Medicine, Taipei Medical University Hospital, Taipei Medical University, Taipei, Taiwan, ROC

    Chi-Ching Chang

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  1. Yow-Jen Lo
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Correspondence to Chien-Ho Chen.

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Lo, YJ., Sheu, MT., Tsai, WC. et al. Intra-articular injection of hyaluronate and indomethacin in rabbits with antigen-induced arthritis. Rheumatol Int 27, 1099–1111 (2007). https://doi.org/10.1007/s00296-007-0346-1

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  • DOI: https://doi.org/10.1007/s00296-007-0346-1

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