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Effect of Decantation Time on Viability and Apoptosis in Adipocytes After Liposuction

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  • Basic Science/Experimental
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Abstract

Background

The effect of decantation time on viability and apoptosis in adipocytes has not been described. The objective of the study was to describe viability and apoptosis in adipocytes up to 2 h after harvesting.

Methods

Twenty patients who underwent esthetic liposuction from the abdomen were included. The lipoaspirate was obtained from the infra-umbilical area with the tumescent technique. Liposuction was performed with a 60-ml syringe and a 3-ml cannula. Lipoaspirates were centrifuged at 50 g for 5 min at 0, 60 and 120 min after harvesting. One gram of fat was digested with 0.1% type 1 collagenase and incubated at 37 degrees for 30 min. Adipocytes were counted on 10 random microscopic fields. Apoptosis was determined by TUNEL assay. A fluorescence microscope was used to visualize the staining nuclei and cells.

Results

Regarding viability, immediately after harvesting, 57.6 ± 18.9% of the cells were viable, whereas 60 min after liposuction the viability decreased to 51.62 ± 8.8% and 120 min after liposuction the percentage of viable cells was 46.8 ± 16.9%. The percentage of apoptotic cells at time 0 was 38.2 ± 8.0%, whereas it was 51.24 ± 8.1% at 60 min and 62.9 ± 16.1% at 120 min after collection.

Conclusions

Apoptosis and mortality of adipocytes after liposuction increase directly proportional to the time of decantation. Lipoinjection should be performed as soon as possible after harvesting.

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Authors and Affiliations

  1. Department of Plastic Surgery, Universidad Autonoma de Nuevo Leon, Madero Ave. And Gonzalitos Ave. Mitras Centro, Monterrey, N.L., Mexico

    Gabriel A. Mecott, Iram Z. Gonzalez, Ivan Gonzalez-Cantu, Yanko Castro-Govea & Mauricio M. García-Pérez

  2. Histology Department, Faculty of Medicine, Universidad Autonoma de Nuevo Leon, Monterrey, Mexico

    Roberto Montes de Oca, Rodolfo Garza-Morales & Odilia Saucedo-Cárdenas

Authors
  1. Gabriel A. Mecott
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  2. Iram Z. Gonzalez
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  3. Roberto Montes de Oca
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  4. Rodolfo Garza-Morales
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  5. Ivan Gonzalez-Cantu
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  6. Yanko Castro-Govea
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  7. Odilia Saucedo-Cárdenas
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  8. Mauricio M. García-Pérez
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Correspondence to Gabriel A. Mecott.

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Mecott, G.A., Gonzalez, I.Z., Montes de Oca, R. et al. Effect of Decantation Time on Viability and Apoptosis in Adipocytes After Liposuction. Aesth Plast Surg 43, 228–232 (2019). https://doi.org/10.1007/s00266-018-1258-z

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  • DOI: https://doi.org/10.1007/s00266-018-1258-z

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