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Botulinum Toxin Enhances the Implantation Effect of Adipocytes in C57/BL6 Mice

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Abstract

Background

Recently, many plastic surgeons have been using adipogenic-differentiated cell implantation for remodeling scars in patients. However, this technique is not a long-term solution because implanted cells disappear gradually. Therefore, we investigated a method to increase the grafted cell preservation rate by using an effective adjuvant, botulinum toxin.

Methods

The adipogenic-differentiated cells were subcutaneously injected in the dorsal area of C57/BL6 mice with or without botulinum toxin. Two and six weeks later we analyzed the residual volume and confirmed the characteristics of the implanted cells by real-time RT-PCR and immunohistochemistry.

Results

Two and six weeks after transplantation we found that the residual volume of the transplantation site was higher in the botulinum toxin-treated group than in the untreated group. We also confirmed that the residual transplanted area has characteristics of adipogenic tissue by histological analysis. Next, to determine the mechanism related to the enhanced preservation rate of grafted cells via treatment with botulinum toxin, we performed immunohistochemical staining for the angiogenesis-related marker CD31. We found that CD31 expression was higher in the botulinum toxin-treated group than in the untreated group.

Conclusion

We have shown that in vivo grafted adipocyte cell preservation can be enhanced by treatment with botulinum toxin as an adjuvant. We suggest that botulinum toxin further increases this graft preservation rate by enhancing angiogenesis.

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Acknowledgments

This work was supported by a Korea Research Foundation grant funded by the Korean Government (MOEHRD) (KRF-2006-005-J02202), a grant from the Korea Health 21 R&D project, Ministry of Health & Welfare, Republic of Korea (A050569), and the Seoul Research and Business Development Program (10582).

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Authors and Affiliations

  1. Department of Life Science, Sookmyung Women’s University, Yongsan-gu, Seoul, 140-742, Korea

    Min Kyung Jung, Seok Bean Song, So Young Cheon, Yoorim Park & Daeho Cho

  2. Department of Dermatology, St. Mary’s Hospital, The Catholic University, Seoul, 137-701, Korea

    Hyunjeong Park

  3. Department of Animal Resource and Sciences, Dankook University, Cheonan, 330-714, Korea

    Cherlhyun Kim

  4. Department of Ophthalmology, School of Medicine, Konkuk University Hospital, Konkuk University, Seoul, 143-701, Korea

    Byung Joo Cho

  5. Department of Anatomy and Tumor Immunity Medical Research Center, Seoul National College of Medicine, Seoul, 110-744, Korea

    Wang Jae Lee

  6. Faculty of Medicine, Imperial College London, London, SW72AZ, UK

    Jung-Wook Bang

  7. Department of Plastic Surgery, College of Medicine, Sungkyunkwan University, Seoul, 110-745, Korea

    Sa Ik Bang

Authors
  1. Min Kyung Jung
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  2. Seok Bean Song
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  3. So Young Cheon
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  4. Yoorim Park
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  5. Hyunjeong Park
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  6. Cherlhyun Kim
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  7. Byung Joo Cho
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  8. Wang Jae Lee
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  9. Jung-Wook Bang
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  10. Sa Ik Bang
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  11. Daeho Cho
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Corresponding authors

Correspondence to Sa Ik Bang or Daeho Cho.

Additional information

Daeho Cho and Sa Ik Bang contributed equally to this work.

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Jung, M.K., Song, S.B., Cheon, S.Y. et al. Botulinum Toxin Enhances the Implantation Effect of Adipocytes in C57/BL6 Mice. Aesth Plast Surg 33, 722–729 (2009). https://doi.org/10.1007/s00266-009-9394-0

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  • DOI: https://doi.org/10.1007/s00266-009-9394-0

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