Skip to main content

Advertisement

SpringerLink
Log in

Expression of mitotic Aurora/Ipl1p-related kinases in renal cell carcinomas: an immunohistochemical study

  • Original Paper
  • Published:
Urological Research Aims and scope Submit manuscript

Abstract

The Aurora/Ipl1p-related kinases, AIRK1, AIRK2 and AIRK3, are members of a novel family of oncogenic serine/threonine kinases regulated by cell cycle progression and involved in chromosome segregation and cytokinesis. In this study, we examined expression of members of the AIRK family in human renal cell carcinomas. Expression of AIRK subfamilies was examined in 64 renal cell carcinomas by immunohistochemistry. Immunostaining of AIRK1, AIRK2 and AIRK3 was observed in 95%, 47% and 98% of specimens, respectively. Moreover, in specimens from the same patient, staining of AIRK2 was correlated with proliferating cell nuclear antigen labeling. Here we provide the first description of AIRK isozyme immunostaining in human renal cell carcinoma. Although the precise physiological functions of these kinases are not known, AIRK subfamily expression may play a role in renal cell carcinoma tumorigenesis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1A–I.
Fig. 2.

Similar content being viewed by others

References

  1. Bischoff JR, Plowman GD (1999) The Aurora/Ipl1p kinase family: regulators of chromosome segregation and cytokinesis. Trends Cell Biol 9: 454

    CAS  PubMed  Google Scholar 

  2. Bischoff JR, Anderson L, Zhu Y, Mossie K, Ng L, Souza B, Schryver B, Flanagan P, Clairvoyant F, Ginther C, Chan CS, Novotny M, Slamon DJ, Plowman GD (1998) A homologue of Drosophila aurora kinase is oncogenic and amplified in human colorectal cancers. EMBO J 17: 3052

    CAS  PubMed  Google Scholar 

  3. Doxsey S (1998) The centrosome—a tiny organelle with big potential. Nat Genet 20: 104

    Article  CAS  PubMed  Google Scholar 

  4. Giet R, Prigent C (1999) Aurora/Ipl1p-related kinases, a new oncogenic family of mitotic serine-threonine kinases. J Cell Sci 112: 3591

    CAS  PubMed  Google Scholar 

  5. Honda K, Mihara H, Kato Y, Yamaguchi A, Tanaka H, Yasuda H, Furukawa K, Urano T (2000) Degradation of human Aurora2 protein kinase by the anaphase-promoting complex-ubiquitin-proteasome pathway. Oncogene 19:2812

    Article  CAS  PubMed  Google Scholar 

  6. Katayama H, Ota T, Jisaki F, Ueda Y, Tanaka T, Odashima S, Suzuki F, Terada Y, Tatsuka M (1999) Mitotic kinase expression and colorectal cancer progression. J Natl Cancer Inst 91: 1160

    Article  CAS  PubMed  Google Scholar 

  7. Kimura M, Kotani S, Hattori T, Sumi N, Yoshioka T, Todokoro K, Okano Y (1997) Cell cycle-dependent expression and spindle pole localization of a novel human protein kinase, Aik, related to Aurora of Drosophila and yeast Ipl1. J Biol Chem 272: 13766

    Article  CAS  PubMed  Google Scholar 

  8. Kimura M, Matsuda Y, Yoshioka T, Sumi N, Okano Y (1998) Identification and characterization of STK12/Aik2: a human gene related to aurora of Drosophila and yeast IPL1. Cytogenet Cell Genet 82: 147

    Article  CAS  PubMed  Google Scholar 

  9. Kimura M, Matsuda Y, Yoshioka T, Okano Y (1999) Cell cycle-dependent expression and centrosome localization of a third human aurora/Ipl1-related protein kinase, AIK3. J Biol Chem 274: 7334

    Article  CAS  PubMed  Google Scholar 

  10. Loeb LA (1998) Cancer cells exhibit a mutator phenotype. Adv Cancer Res 72: 25

    CAS  PubMed  Google Scholar 

  11. Pihan GA, Purohit A, Wallace J, Knecht H, Woda B, Quesenberry P, Doxsey SJ (1998) Centrosome defects and genetic instability in malignant tumors. Cancer Res 58: 3974

    CAS  PubMed  Google Scholar 

  12. Sobin LH, Wittekind CH (1997) Urological tumours. In: TNM classification of malignant tumours, 5th ed, John Wiley & Sons, Inc, New York, p 180

  13. Tanaka T, Kimura M, Matsunaga K, Fukada D, Mori H, Okano Y (1999) Centrosomal kinase AIK1 is overexpressed in invasive ductal carcinoma of the breast. Cancer Res 59: 2041

    CAS  PubMed  Google Scholar 

  14. Tatsuka M, Katayama H, Ota T, Tanaka T, Odashima S, Suzuki F, Terada Y (1998) Multinuclearity and increased ploidy caused by overexpression of the aurora- and Ipl1-like midbody-associated protein mitotic kinase in human cancer cells. Cancer Res 58: 4811

    CAS  PubMed  Google Scholar 

  15. Terada Y, Tatsuka M, Suzuki F, Yasuda Y, Fujita S, Otsu M (1998) AIM-1: a mammalian midbody-associated protein required for cytokinesis. EMBO J 17: 667

    CAS  PubMed  Google Scholar 

  16. Zhou H, Kuang J, Zhong L, Kuo WL, Gray JW, Sahin A, Brinkley BR, Sen S (1998) Tumour amplified kinase STK15/BTAK induces centrosome amplification, aneuploidy and transformation. Nat Genet 20: 189

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Urology, Gifu University School of Medicine, 40 Tsukasa, Gifu 500-8705, Japan

    Hidetoshi Ehara, Shigeaki Yokoi, Masayoshi Tamaki, Yoshinori Nishino, Yoshito Takahashi & Takashi Deguchi

  2. Department of Molecular Pathobiochemistry, Gifu University School of Medicine, 40 Tsukasa, Gifu 500-8705, Japan

    Masashi Kimura, Takashi Yoshioka & Yukio Okano

Authors
  1. Hidetoshi Ehara
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Shigeaki Yokoi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Masayoshi Tamaki
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yoshinori Nishino
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yoshito Takahashi
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Takashi Deguchi
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Masashi Kimura
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Takashi Yoshioka
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Yukio Okano
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hidetoshi Ehara.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ehara, H., Yokoi, S., Tamaki, M. et al. Expression of mitotic Aurora/Ipl1p-related kinases in renal cell carcinomas: an immunohistochemical study . Urol Res 31, 382–386 (2003). https://doi.org/10.1007/s00240-003-0354-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00240-003-0354-x

Keywords

Search

Navigation