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Grape seed proanthocyanidin extract inhibits interleukin-17-induced interleukin-6 production via MAPK pathway in human pulmonary epithelial cells

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Abstract

This study was aimed to investigate the effect of grape seed proanthocyanidin extract (GSPE) on interleukin-17 (IL-17)-induced interleukin-6 (IL-6) production in A549 human pulmonary epithelial cells. Cells were treated with IL-17 (10 ng/ml) or GSPE (50 μg/ml), or both. The effects of GSPE on cell viability and apoptosis were evaluated. The mRNA and protein levels of IL-6 were determined by reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. Expressions of inhibitory factor κB-α (IκB-α) and ERK 1/2, p38, and JNK mitogen-activated protein kinase (MAPK) were examined by Western blot analysis. GSPE significantly inhibited both GSPE treatment only and IL-17-induced mRNA expressions and protein productions of IL-6 in pulmonary epithelial cells (p < 0.01). GSPE decreased the IL-17-induced phosphorylation of IκB-α. The IL-17 stimulated ERK 1/2, p38, and JNK MAPK activities and GSPE decreased IL-17-stimulated ERK 1/2, p38, and JNK MAPK activities. GSPE also attenuated TNF-α and IL-1β-induced IL-6 productions (p < 0.05). Our results show that GSPE may inhibit IL-17-stimulated IL-6 productions in human pulmonary epithelial cells by inhibiting MAPK and nuclear factor-κB-mediated signaling pathway.

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Acknowledgments

The present research has been conducted by attached research institute research grant of Keimyung University in 2007.

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Authors and Affiliations

  1. Department of Biochemistry, Pain Research Center, School of Medicine, Keimyung University, Daegu, 704–701, Republic of Korea

    Hyeongseok Kim, Kyo-Chul Mun & Eunyoung Ha

  2. Department of Internal Medicine and Pain Research Center, School of Medicine, Keimyung University Dongsan Medical Center, Daegu, 700–712, Republic of Korea

    Jin Young Kim, Hong Suk Song & Keon Uk Park

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  1. Hyeongseok Kim
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Correspondence to Eunyoung Ha.

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Hyeongseok Kim and Jin Young Kim contributed equally to this work.

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Kim, H., Kim, J.Y., Song, H.S. et al. Grape seed proanthocyanidin extract inhibits interleukin-17-induced interleukin-6 production via MAPK pathway in human pulmonary epithelial cells. Naunyn-Schmiedeberg's Arch Pharmacol 383, 555–562 (2011). https://doi.org/10.1007/s00210-011-0633-y

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