Abstract
Introduction and hypothesis
We assessed whether urinary excretion of glycosaminoglycans (GAGs) may be affected by a condition of detrusor overactivity (DO).
Materials and methods
We evaluated 24-h urinary excretion of GAGs in 25 patients (mean age 63.81 years) with DO and in 14 healthy controls (mean age 65.75 years). No patients or controls had urinary tract infection. The excretion of GAGs was measured on the basis of total urine volume and normalized to creatinine concentration. Student’s test was employed to check between the urinary excretion of GAGs in the two groups.
Results
The urinary content of GAGs discovered in control group was significantly higher than that in pathological group for the ratio GAGs/creatinine concentration.
Conclusions
The low content of GAGs might be due to relative ischemia of the bladder wall epithelial layer following a chronically increased contractile activity of the detrusor even if further studies are needed to confirm this finding.
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References
Hurst RE, Roy JB, Min KW, Veltri RW, Marley G, Patton et al (1996) A deficit of chondroitin sulfate proteoglycans on the bladder uroepithelium in interstitial cystitis. Urology 48:817–821
Parsons CL, Stauffer C, Schmidt JD (1980) Bladder-surface glycosaminoglycans: an efficient mechanism of environmental adaptation. Science 208:605–607
Bodenstab W, Kaufman J, Parsons CL (1983) Inactivation of antiadherence effect of bladder surface glycosaminoglycan by a complete urinary carcinogen (N-methyl-N-nitrosourea). J Urol 129:200–201
Hook M, Kjellen L, Johansson S (1984) Cell-surface glycosaminoglycans. Annu Rev Biochem 53:847–869
Hurst RE, Rhodes SW, Adamson PB, Parsons CL, Roy JB (1987) Functional and structural characteristics of the glycosaminoglycans of the bladder luminal surface. J Urol 138:433–437
Lokeshwar VB, Selzer MG, Unwala DJ, Estrella V, Gomez MF, Golshani R et al (2006) Uronate peaks and urinary hyaluronic acid levels correlate with interstitial cystitis severity. J Urol 176:1001–1007
Hurst RE, Parsons CL, Roy JB, Young JL (1993) Urinary glycosaminoglycan excretion as a laboratory marker in the diagnosis of interstitial cystitis. J Urol 9:31–35
Ferrara P, Marrone G, Mastrangelo A, Nicoletti A, Emmanuele V, Fasano A (2007) Increased excretion of glycosaminoglycans in children with urinary incontinence compared to those with monosymptomatic nocturnal enuresis. Scand J Urol Nephrol 41:218–222
Haylen BT, Ashby D, Sutherst JR, Frazer MI, West CR (1989) Maximum and average urine flow rates in normal male and female populations the Liverpool nomograms. Br J Urol 64:30–38
Abrams P, Cardozo L, Fall M, Griffiths D, Ulmsten R et al (2002) The standardisation of terminology of lower urinary tract function : report from the standardisation sub-committee of the International Continence Society. Neurourol Urodyn 21:167–178
Hesse A, Wuzel H, Vahlensieck W (1986) The excretion of glycosaminoglycans in the urine of calcium-oxalate-stone patients and healthy persons. Urol Int 41:81–87
Bitter T, Muir HM (1962) A modified uronic acid carbazole reaction. Anal Biochem 4:330–334
Sprent P, Smeeton NC (2001) Applied nonparametric statistical methods, 3rd edn. Chapman & Hall, London IX + 459 p
Hauser PJ, Dozmorov MG, Bane BL, Slobodov G, Culkin DJ, Hurst RE (2008) Abnormal expression of differentiation related proteins and proteoglycan core proteins in the urothelium of patients with interstitial cystitis. J Urol 179:764–769
Hennessey PT, Hurst RE, Hemstreet GP 3rd, Cutter G (1981) Urinary glycosaminoglycan excretion as a biochemical marker in patients with bladder carcinoma. Cancer Res 41:3868–3873
Salvaggio E, Antuzzi D, Ferrara P, Scanu M, Pulitanò S, Venuti L et al (2001) Glycosaminoglycans: urinary excretion in children with myelomeningocele. Urol Int 66:156–159
Erickson DR, Xie SX, Bhavanandan VP, Wheeler MA, Hurst RE, Demers LM et al (2002) A comparison of multiple urine markers for interstitial cystitis. J Urol 167:2461
Soler R, Bruschini H, Martins JR, Dreyfuss JL, Camara NO, Alves MT et al (2008) Urinary glycosaminoglycans as biomarker for urothelial injury: is it possible to discriminate damage from recovery? Urology 72:937–942
Parsons CL (1996) Potassium sensitività test. Tech Urol 2:171–173
Daha LK, Riedl CR, Hohlbrugger G, Knoll M, Engelhardt PF, Pfluger H (2003) Comparative assessment of maximal bladder capacity, 0.9% NaCl versus 0.2 M KCl, for the diagnosis of interstitial cystitis: a prospective controlled study. J Urol 170:807–809
Brading AF (1997) A myogenic basis for the overactive bladder. Urology 50:57–67
Levin RM, Brendler K, Van Arsdalen KN, Wein AJ (1983) Functional response of the rabbit urinary bladder to anoxia and ischaemia. Neurouroll Urodyn 2:233–243
Zhao Y, Wein AJ, Bilgen A, Levin RM (1991) Effect of anoxia on in vitro bladder function. Pharmacology 43:337–344
Pessina F, McMurray G, Wiggin A, Brading AF (1997) The effect of anoxia and glucose-free solutions on the contractile response of guinea-pig detrusor strips to intrinsic nerve stimulation and the application of excitatory agonists. J Urol 157:2375–2380
Hedbom E, Hauselmann HJ (2002) Molecular aspects of pathogenesis in osteoarthritis: the role of inflammation. Cell Mol Life Sci 59:45–53
Lotz M (2001) Cytokines in cartilage injury and repair. Clin Orthop 391:S108–S115
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Siracusano, S., Cucchi, A., Ciciliato, S. et al. Urinary levels of glycosaminoglycans in patients with idiopathic detrusor overactivity. Int Urogynecol J 20, 1477–1480 (2009). https://doi.org/10.1007/s00192-009-0973-z
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DOI: https://doi.org/10.1007/s00192-009-0973-z