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High-resolution mapping of zym, a recessive gene for Zucchini yellow mosaic virus resistance in cucumber

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Using a high-resolution mapping approach, we identified a candidate gene for ZYMV resistance in cucumber. Our findings should assist the development of high-versatility molecular markers for MAS for ZYMV resistance.

Abstract

Zucchini yellow mosaic virus (ZYMV) causes significant disease, which leads to fruit yield loss in cucurbit crops. Since ZYMV resistance is often inherited recessively in cucumber, marker-assisted selection (MAS) is a useful tool for the development of resistant cucumber cultivars. Using 128 families of an F2:3 population derived from a cross between susceptible ‘CS-PMR1’ and resistant ‘A192-18’ cucumber inbred lines, we confirmed that ZYMV resistance is conferred by a single recessive locus: zym A192-18. We constructed a cucumber genetic linkage map that included 125 simple sequence repeat (SSR) markers segregating into 7 linkage groups (chromosomes). The zym A192-18 locus was mapped to chromosome 6, at genetic distances of 0.9 and 1.3 cM from two closely linked SSR markers. For high-resolution genetic mapping, we identified new molecular markers cosegregating with the zym A192-18 locus; using cucumber genomic and molecular marker resources and screening an F2 population of 2,429 plants, we narrowed down the zym A192-18 locus to a <50-kb genomic region flanked by two SSR markers, which included six candidate genes. Sequence analysis of the candidate genes’ coding regions revealed that the vacuolar protein sorting-associated protein 4-like (VPS4-like) gene had two SNPs between the parental lines. Based on SNPs of the VPS-4-like gene, we developed zym A192-18-linked DNA markers and found that genotypes associated with these markers were correlated with the ZYMV resistance phenotype in 48 cucumber inbred lines. According to our data, the gene encoding VPS4-like protein is a candidate for the zym A192-18 locus. These results may be valuable for MAS for ZYMV resistance in cucumber.

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Acknowledgments

The authors wish to thank Professor Dr. Tomohide Natsuaki (Utsunomiya University, Japan) for kindly providing the ZYMV Z5-1 strain. The authors are also grateful to Dr. Nobuko Fukino (National Institute of Vegetable and Tea Science, Japan) for her valuable suggestions regarding linkage map construction.

Conflict of interest

The authors have declared no conflicts of interest.

Author information

Authors and Affiliations

  1. Saitama Gensyu Ikuseikai Co. Ltd., Kuki, Saitama, 346-0105, Japan

    Masashi Amano & Akira Mochizuki

  2. Department of Plant Science and Resources, College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa, 252-0880, Japan

    Yumi Kawagoe, Keisuke Iwahori, Kaori Niwa, Takanori Maeda & Yoshiyuki Imura

  3. Crop Research Institute, Drnovská 507, 16106, Prague 6, Czech Republic

    Jiri Svoboda

Authors
  1. Masashi Amano
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  2. Akira Mochizuki
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  3. Yumi Kawagoe
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  4. Keisuke Iwahori
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  5. Kaori Niwa
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  6. Jiri Svoboda
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  7. Takanori Maeda
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  8. Yoshiyuki Imura
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Corresponding author

Correspondence to Masashi Amano.

Additional information

Communicated by I. Paran.

DDBJ accession numbers for sequences reported in this manuscript are AB819727–AB819730.

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Amano, M., Mochizuki, A., Kawagoe, Y. et al. High-resolution mapping of zym, a recessive gene for Zucchini yellow mosaic virus resistance in cucumber. Theor Appl Genet 126, 2983–2993 (2013). https://doi.org/10.1007/s00122-013-2187-5

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  • DOI: https://doi.org/10.1007/s00122-013-2187-5

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