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Mapping quantitative trait loci conferring resistance to rice black-streaked virus in maize (Zea mays L.)

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Abstract

Maize rough dwarf disease (MRDD) is one of the most serious virus diseases of maize worldwide, and it causes great reduction of maize production. In China, the pathogen was shown to be rice black-streaked virus (RBSDV). Currently, MRDD has spread broadly and leads to significant loss in China. However, there has been little research devoted to this disease. Our aims were to identify the markers and loci underlying resistance to this virus disease. In this study, segregation populations were constructed from two maize elite lines ‘90110’, which is highly resistant to MRDD and ‘Ye478’, which is highly susceptible to MRDD. The F2 and BC1 populations were used for bulk sergeant analysis (BSA) to identify resistance-related markers. One hundred and twenty F7:9 RILs were used for quantitative trait loci (QTL) mapping through the experiment of multiple environments over 3 years. Natural occurrence and artificial inoculation were both used and combined to determine the phenotype of plants. Five QTL, qMRD2, qMRD6, qMRD7, qMRD8 and qMRD10 were measured in the experiments. The qMRD8 on chromosome 8 was proved to be one major QTL conferring resistance to RBSDV disease in almost all traits and environments, which explained 12.0–28.9 % of the phenotypic variance for disease severity in this present study.

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Acknowledgments

This work was supported by the National Basic Research Program of China (973 Program 2009CB118400) and National High Technology Research and Development Program of China (863 Program) (No. 2012AA10A306). We thank Professor Deng-hai Li (Laizhou Academy of Agricultural Sciences) for donating maize inbred line ‘Ye478’ and supporting to field tests. We thank Professor Cheng-he Zhang (Hebei Academy of Agricultural Sciences) for donating maize inbred line ‘90110’. We are grateful to Doctor Roberta Greenwood for help with editing the English of the paper.

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Authors and Affiliations

  1. School of Life Sciences, Shandong University, Jinan, China

    Junwen Luan, Fei Wang, Yujie Li, Bin Zhang & Juren Zhang

  2. School of Life Sciences, Shanghai University, Shanghai, China

    Fei Wang

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  1. Junwen Luan
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  2. Fei Wang
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  3. Yujie Li
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Correspondence to Juren Zhang.

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Communicated by M. Xu.

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Luan, J., Wang, F., Li, Y. et al. Mapping quantitative trait loci conferring resistance to rice black-streaked virus in maize (Zea mays L.). Theor Appl Genet 125, 781–791 (2012). https://doi.org/10.1007/s00122-012-1871-1

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  • DOI: https://doi.org/10.1007/s00122-012-1871-1

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