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Serum copper/zinc superoxide dismutase levels in patients with rheumatoid arthritis

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International Journal of Clinical and Laboratory Research

Abstract

Rheumatoid arthritis is characterized by a chronic hypertrophic synovitis leading to destruction of connective tissue and functional damage of cartilage and bone structures. Reactive oxygen species play an important role in tissue injury in this disease. To clarify the role of the cellular antioxidant system in the protection against oxygen free radicals, we examined the levels of copper/zinc superoxide dismutase in the sera of patients with rheumatoid arthritis. We used an enzyme-linked immunosorbent assaywhich determines the concentration of copper/zinc superoxide dismutase indepedently from its enzymatic activity. We found that patients with rheumatoid arthritis have higher serum copper/zinc superoxide dismutase levels than control subjects. Copper/zinc superoxide dismutase also correlated positively with serum levels of both neopterin and rheumatoid factor, sensitive markers for disease activity in rheumatoid arthritis. These results support the hypothesis that the increased amount of copper/zinc superoxide dismutase is probably inadequate to exert an effective antioxidant protection but can result in a pro-inflammatory, pathogenic effect enhancing tissue damage. Furthermore, copper/zinc superoxide dismutase might be used as a marker of inflammatory activity in rheumatoid arthritis.

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References

  1. Bodman KB, Roitt IM. The pathophysiology of rheumatoid arthritis. Fund Am Clin Immunol 1994;2: 73.

    Google Scholar 

  2. Lunec J. Oxygen radicals: their measurement and role in major disease. JIFCC 1992; 4: 58.

    CAS  Google Scholar 

  3. Blake DR, Merry P, Unsworth J, Outhwait J, Morris CJ, Gray L, Lunec J. Hyoxic-reperfusion injury in the inflamed human joint. Lancet 1989; 1: 289.

    Article  PubMed  CAS  Google Scholar 

  4. Harris ED. Regulation of antioxidant enzymes. FASEB J 1992; 6: 2675.

    PubMed  CAS  Google Scholar 

  5. Panasyuk A, Frati E, Ribault D, Mitrovic DJ. Effect of reactive oxygen species on the biosynthesis and structure of newly synthesized proteoglycans. Free Radic Biol Med 1994; 16: 157.

    Article  PubMed  CAS  Google Scholar 

  6. Greenwald RA, Moy WW. Effect of oxygen free radicals on hyaluronic acid. Arthritis Rheum 1980; 23: 445.

    Article  Google Scholar 

  7. Buettner GR, Chamulitrat W. The catalytic activity of iron in synovial fluid as monitored by the ascorbate free radical. Free Radic Biol Med 1990; 8: 55.

    Article  PubMed  CAS  Google Scholar 

  8. Porstmann T, Wietschke R, Schmechta H, Grunow R, Porstmann B, Bleiber R, Pergande M, Stachat S, Baehr R von. A rapid and sensitive enzyme-immunoassay for Cu/Zn superoxide dismutase with polyclonal and monoclonal antibodies. Clin Chim Acta 1988; 171: 1.

    Article  PubMed  CAS  Google Scholar 

  9. Igari T, Kaneda H, Horiuchi S, Ono SA. A remarkable increase of superoxide dismutase activity in synovial fluid of patients with rheumatoid arthritis. Clin Orthop 1982; 162: 282.

    PubMed  Google Scholar 

  10. Blake DR, Hall ND, Terby DA, Halliwell B, Gutteridge JMC. Protection against superoxide and hydrogen peroxide in synovial fluid from rheumatoid patients. Clin Sci 1981; 61: 483.

    PubMed  CAS  Google Scholar 

  11. Biemond P, Swaak AJG, Koster JF. Prorective factors against hydrogen peroxide in rheumatoid arthritis synovial fluid. Arthritis Rheum 1984; 27: 760.

    Article  PubMed  CAS  Google Scholar 

  12. Pham Huu T, Marquetty C, Amit N, Hakim JJ. Effect of degranulation on superoxide dismutase activity in human neutrophils. Free Radic Biol Med 1986; 2: 213.

    Article  CAS  Google Scholar 

  13. Arnett FC, Edworthy SM, Bloch DA, McShane DJ, Fries JF, Cooper NS, Healey LA, Kaplan SR, Liang MH, Luthra HS, Medsger TA Jr, Mitchell DM, Neustadt DH, Pinals RS, Schaller JC, Sharp JT, Wilder RL, Hunder GG. The American Rheumatism Association 1987 revised criteria for the classification of rheumatoid arthritis. Arthritis Rheum 1988; 31: 315.

    Article  PubMed  CAS  Google Scholar 

  14. Porstmann T, Wietschke R, Grunow R, Jahn S, Porstmann B, Schmechta H, Pergande M, Baehr R von. Production and characterization of monoclonal antibodies against human Cu/Zn superoxide dismutase and the establishment of a super-rapid enzyme-linked immunosorbent assay (SURALISA). J Immunol Methods 1990; 127: 1.

    Article  PubMed  CAS  Google Scholar 

  15. Schmechta H. Enzymimmunassay für Haemoglobin in der Blutspurenkunde. I. Mitteilung: Erzeugung von anti-human-Haemoglobin und Testaufbau Krim. Foren Wiss 1990; 77–78: 124.

    Google Scholar 

  16. Zuckerman SH, Suprenant YM. Simplified microELISA for the quantitation of murine serum amyloid A protein. J Immunol Methods 1986; 92: 37.

    Article  PubMed  CAS  Google Scholar 

  17. Lund-Oleson K. Oxygen tensions in synovial fluids. Arthritis Rheum 1970; 13: 769.

    Article  Google Scholar 

  18. Edwards SW, Hallet MB, Campbell AK. Oxygen radical production may be limited by oxygen concentration. Biochem J 1984; 217: 851.

    PubMed  CAS  Google Scholar 

  19. Henrotin Y, Deby-Dupont G, Deby C, De Bruyn M, Lamy M, Franchimont P. Production of active oxygen species by isolated human chondrocytes. Comput Chem 1993; 32: 562.

    CAS  Google Scholar 

  20. Blake DR, Gallagher PJ, Potter AR. The effect of synovial iron on the progression of rheumatoid disease: an histological assessment of patient with early rheumatoid synovitis. Arthritis Rheum 1984; 27: 495.

    Article  PubMed  CAS  Google Scholar 

  21. Mao GD, Thomas PD, Lopaschuk GD, Poznansky MJ. Superoxide dismutase (SOD)-catalase conjugates. Role of hydrogen peroxide and the Fenton reaction in SOD toxicity. J Biol Chem 1993; 268: 416.

    PubMed  CAS  Google Scholar 

  22. Yim MB, Chock PB, Stadtman ER. Cu/Zn superoxide dismutase catalyzes hydroxyl radical production from hydrogen peroxide. Proc Natl Acad Sci USA 1990; 87: 5006.

    Article  PubMed  CAS  Google Scholar 

  23. Lynch RE, Fridovich IJ. Effects of superoxide on the erythrocytes membrane. Biol Chem 1978; 253: 1838.

    CAS  Google Scholar 

  24. Misra HP, Fridovich I. Superoxide dismutase “positive” spectrophotometric assay. Anal Biochem 1977; 79: 553.

    Article  PubMed  CAS  Google Scholar 

  25. Fuchs D, Hausen A, Reibnegger G, Werner ER, Dietrich MP, Watcher H. Neopterin as a marker for activated cell-mediated immunity: application in HIV infection. Immunol Today 1988; 9: 150.

    Article  PubMed  CAS  Google Scholar 

  26. Hannonen P, Sari Tikanoja, Hakola M, Mottonen T, Viinikka L, Oka M. Urinary neopterin index as a measure of rheumatoid activity. Scand J Rheumatol 1986; 15: 148.

    PubMed  CAS  Google Scholar 

  27. Reibnegger G, Egg D, Fuchs D, Günther R, Hausen A, Werner ER, Wachter H, Urinary neopterin reflects clinical activity in patients with rheumatoid arthritis. Arthritis Rheum 1986; 29: 1063.

    Article  PubMed  CAS  Google Scholar 

  28. Kullich W. Correlation of interleukin-2 receptor and neopterin secretion in rheumatoid arthritis. Clin Rheumatol 1993; 12: 387.

    Article  PubMed  CAS  Google Scholar 

  29. Borzì RM, Grigolo B, Meliconi R, Fasano L, Sturani C, Fabbri M, Porstmann T, Facchini A. Elevated serum superoxide dismutase levels correlate with disease severity and neutrophil degranulation in idiopathic pulmonary fibrosis. Clin Sci 1993; 85: 353.

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Laboratorio di Immunologia e Genetica, Istituto di Ricerca Codivilla Putti, Istituti Ortopedici Rizzoli, Via di Barbiano 1/10, 1-40136, Bologna, Italy

    I. Mazzetti, B. Grigolo, R. M. Borzì & A. Facchini

  2. Dipartimento di Medicina Interna, Cardioangiologia, Epatologia, Universita degli Studi di Bologna, Bologna, Italy

    R. Meliconi

  3. Dipartimento di Medicina Interna e Gastroenterologia, Università degli Studi di Bologna, Bologna, Italy

    A. Facchini

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  1. I. Mazzetti
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  2. B. Grigolo
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  3. R. M. Borzì
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  4. R. Meliconi
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  5. A. Facchini
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Mazzetti, I., Grigolo, B., Borzì, R.M. et al. Serum copper/zinc superoxide dismutase levels in patients with rheumatoid arthritis. Int J Clin Lab Res 26, 245–249 (1996). https://doi.org/10.1007/BF02602957

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  • DOI: https://doi.org/10.1007/BF02602957

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