Abstract
In an effort to initiate steps designed to characterize the idiopathic hemochromatosis disease gene, theHLA-A/HLA-F region where this gene is in disequilibrium linkage with some polymorphic markers has been over-lapped by a yeast artificial chromosome (YAC) contig. In order to achieve the physical mapping of these YACs and of the corresponding genomic region, we subcloned one of the YACs involved. A computer-assisted analysis of the sequence of one subclone led to the isolation of a potential exon that proved to belong to a new expressed messenger namedHCGIX. After southern blot analysis, the corre-sponding cDNA clone was found to belong to a new multigene family whose members are dispersed throughout theHLA class I region and are closely associated with members of another recently described multigene family designatedPERB11. The data reported here suggest that these two multigene families form a cluster that have been dispersed together throughout the telomeric part of the major histocompatibility complex and have been involved in the genesis of this human class I region.
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The nucleotide sequence data reported in this paper have been submitted to the EMBL nucleotide sequence database and have been assigned the accession numbers X92109, X95288, and X95289
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Pichon, L., Hampe, A., Giffon, T. et al. A new non-HLA multigene family associated with thePERB11 family within theMHC class I region. Immunogenetics 44, 259–267 (1996). https://doi.org/10.1007/BF02602555
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DOI: https://doi.org/10.1007/BF02602555