Abstract
During an 18-month period all stools submitted to a microbiology laboratory in Belgium for culture were screened for VerocytotoxinproducingEscherichia coli (VTEC) serotype O157. In the stool samples from 3940 patients, eight (0.2 %) VTEC O157 strains were isolated, seven of which were O157:H7. Additional screening for other serotypes of VTEC in 332 selected stool samples yielded four more strains (serotypes O2:K1:H6, O111:H−, O117:K1:H7 and O“C70/86”:H−). The 0.3 % isolation rate for all VTEC was comparable to that forShigella spp. Eight children under 30 months and two adults suffered from uncomplicated gastroenteritis. A 5-month-old child and a 41-year-old woman presented with hemolytic uremic syndrome a few days after onset of a diarrheal episode.
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Johnson WM, Lior H, Bezanson GS: CytotoxicEscherichia coli O157:H7 associated with haemorrhagic colitis in Canada. Lancet 1983, i: 76.
Karmali MA, Steele BT, Petric M, Lim C: Sporadic cases of haemolytic-uraemic syndrome associated with faecal cytotoxin and cytotoxin-producingEscherichia coli in stools. Lancet 1983, i: 619–620.
Ramsey PG, Neill MA: Thrombotic thrombocytopenic purpura associated withEscherichia coli O157:H7 — Washington. Morbidity and Mortality Weekly Report 1986, 35: 549–551.
Smith HR, Gross RJ, Rowe B, Fry NK, Scotland SM: Haemorrhagic colitis and Verocytotoxin-producingEscherichia coli in England and Wales. Lancet 1987, i: 1062–1065.
Scotland SM, Rowe B, Smith HR, Willshaw GA, Gross RJ: Verocytotoxin-producing strains ofEscherichia coli from children with haemolytic uraemic syndrome and their detection by specific DNA probes. Journal of Medical Microbiology 1988, 25: 237–243.
Walker CW, Upson R, Warren RE: Haemorrhagic colitis: detection of Verotoxin-producingEscherichia coli O157 in a clinical microbology laboratory. Journal Clinical Pathology 1988, 41: 80–84.
Taylor CM, White RHR, Winterborn MH, Rowe B: Haemolytic-uraemic syndrome: clinical experience of an outbreak in the West Midlands. British Medical Journal 1986, 292: 1513–1516.
Morgan GM, Newman C, Palmer SR, Allen JB, Shepherd W, Rampling AM, Warren RE, Gross RJ, Scotland SM, Smith HR: First recognized community outbreak of haemorrhagic colitis due to Verotoxin-producingEscherichia coli O157:H7 in the UK. Epidemiology and Infections 1988, 101: 83–91.
Karch H, Heesemann J, Laufs R: Phage-associated cytotoxin production by and enteroadhesiveness of enteropathogenicEscherichia coli isolated from infants with diarrhea in West Germany. Journal of Infectious Diseases 1987, 155: 707–715.
Robaeys G, Surmont I, Lemmens P, Coremans G, Vantrappen G, Vandepitte J: Haemorrhagic colitis and Verotoxin-producingEscherichia coli O157 in Belgium. Lancet 1987, i: 1495–1496.
Bitzan M, Karch H, Altrogge H, Strehlau J, Bläker F: Hemolytic-uremic syndrome associated with a variant Shiga-like cytotoxin ofEscherichia coli O111. Pediatric Infectious Disease Journal 1988, 7: 128–132.
March SB, Ratnam S: Sorbitol-MacConkey medium for detection ofEscherichia coli O157:H7 associated with hemorrhagic colitis. Journal of Clinical Microbiology 1986, 23: 869–872.
Farmer JJ, Davis BR: H7 antiserum-sorbitol fermentation medium: a single tube screening medium for detectingEscherichia coli O157:H7 associated with hemorrhagic colitis. Journal of Clinical Microbiology 1985, 22: 620–625.
Karmali MA: Laboratory diagnosis of Verotoxin-producingEscherichia coli infections. Clinical Microbiology Newsletter 1987, 9: 65–70.
Ratnam S, March SB, Ahmed R, Bezanson GS, Kasatiya S: Characterization ofEscherichia coli serotype O157:H7. Journal of Clinical Microbiology 1988, 26: 2006–2012.
Bopp CA, Greene KD, Dowes FP, Sowers EG, Wells JG, Wachsmuth IK: Unusual VerotoxinproducingEscherichia coli associated with hemorrhagic colitis. Journal of Clinical Microbiology 1987, 25: 1486–1489.
Karmali MA: Infection by Verocytotoxin-producingEscherichia coli. Clinical Microbiology Reviews 1989, 2: 15–38.
Gransden WR, Damm MAS, Anderson JD, Carter JE, Lior H: Further evidence associating hemolytic uremic syndrome with infection by Verotoxin-producingEscherichia coli O157:H7. Journal of Infectious Diseases 1986, 154: 522–524.
Harris AA, Kaplan RL, Goodman LJ, Doyle M, Landau W, Segreti J, Mayer K, Levin S: Results of a screening method used in a 12-month stool survey forEscherichia coli O157:H7. Journal of Infectious Diseases 1985, 152: 775–777.
Cahoon FE, Thompson JS: Frequency ofEscherichia coli O157:H7 isolation from stool specimens. Canadian Journal of Microbiology 1987, 33: 914–915.
Pai CH, Ahmet N, Lior H, Johnson WM, Sims HV, Woods DE: Epidemiology of sporadic diarrhea due to Verocytotoxin-producingEscherichia coli: a two-year prospective study. Journal of Infectious Diseases 1988, 157: 1054–1057.
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Piérard, D., Van Etterijck, R., Breynaert, J. et al. Results of screening for verocytotoxin-producingEscherichia coli in faeces in Belgium. Eur. J. Clin. Microbiol. Infect. Dis. 9, 198–201 (1990). https://doi.org/10.1007/BF01963837
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DOI: https://doi.org/10.1007/BF01963837