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Development of a new, more sensitive immunoassay for human tryptase: Use in systemic anaphylaxis

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Abstract

Tryptase, a neutral protease, is selectively concentrated in the secretory granules of human mast cells, and its release into the circulation serves as a clinical marker of mast cell activation. The current study describes a new, more sensitive ELISA utilizing a newly developed, mouse monoclonal IgG1 antibody for capture called B12 and capable of detecting tryptase in normal plasma and serum. The greater sensitivity of the new immunoassay results in part from a greater portion of tryptase being detected. Mean levels of tryptase in serum from normal subjects from Richmond, Virginia (4.9 ng/ml;n=56), Munich, Germany (3.8 ng/ml;n=19), and Amersfoort, The Netherlands (1.9 ng/ml;n=8) were as indicated. In 62 subjects with ongoing allergic rhinitis, tryptase levels were no different in serum than for 19 normal controls, indicating that local mast cell activation is not necessarily reflected in the circulation. In 61 subjects sensitive to honey bee or yellow jacket venom by history, the 17 destined to have a severe, hypotensive response to a sting challenge had higher levels of tryptase at baseline than mild reactors, nonreactors, and controls, suggesting that baseline levels of tryptase may predict the severity of the clinical response to allergen in sensitive subjects.

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Authors and Affiliations

  1. Department of Internal Medicine, Medical College of Virginia, Virginia Commonwealth University, 23298, Richmond, Virginia

    Lawrence B. Schwartz, Timothy R. Bradford & Cheryl Rouse

  2. Department of Pediatrics, Medical College of Virginia, Virginia Commonwealth University, 23298, Richmond, Virginia

    Anne-Marie Irani

  3. Department of Otorhinolaryngology, Ludwig-Maximilians-Universität München, Munich, Bavaria, Federal Republic of Germany

    Gerd Rasp

  4. Department of Internal Medicine, University Hospital Utrecht, Utrecht, The Netherlands

    J. K. Van Der Zwan & Peter-Willem G. Van Der Linden

  5. Departments of Internal Medicine and Clinical Chemistry, Lichtenberg Hospital, Amersfoort, The Netherlands

    Peter-Willem G. Van Der Linden

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  1. Lawrence B. Schwartz
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  2. Timothy R. Bradford
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  4. Anne-Marie Irani
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  6. J. K. Van Der Zwan
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  7. Peter-Willem G. Van Der Linden
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Schwartz, L.B., Bradford, T.R., Rouse, C. et al. Development of a new, more sensitive immunoassay for human tryptase: Use in systemic anaphylaxis. J Clin Immunol 14, 190–204 (1994). https://doi.org/10.1007/BF01533368

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  • DOI: https://doi.org/10.1007/BF01533368

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