Skip to main content
SpringerLink
Log in

Complete nucleotide sequence of sugarcane streak Monogeminivirus

  • Original Papers
  • Published:
Archives of Virology Aims and scope Submit manuscript

Summary

The complete nucleotide sequence of the genome of the Monogeminivirus sugarcane streak virus (SSV) was determined from cloned replicative form DNA. The genome is contained in one DNA circle of 2 758 nucleotides, and has four open reading frames with the potential to encode proteins of MW >10 kDa: two in the viral (+) sense and two in the complementary (−) sense. Each open reading frame has a counterpart among the open reading frames reported for other Monogeminiviruses. A potential binding site for a DNA replication primer and potential transcriptional control sequences were identified on the (+) strand, and a possible intron on the (−) strand. Phylogenetic analysis of coat protein and replication-associated protein sequences of SSV and other grass-infecting geminiviruses indicate that SSV, although distinct from any other virus, is part of an “African streak virus subgroup” of Monogeminiviruses.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Accotto GP, Donson J, Mullineaux PM (1989) Mapping ofDigitaria streak virus transcripts reveals different RNA species from the same transcription unit. EMBO J 8: 1033–1039

    Google Scholar 

  2. Andersen MT, Richardson KA, Harbison S-A, Morris BAM (1988) Nucleotide sequence of the geminivirus chloris striate mosaic virus. Virology 164: 443–449

    Google Scholar 

  3. Boulton MI, Steinkellner H, Donson J, Markham PG, King DI, Davies JW (1989) Mutational analysis of the virion-sense genes of maize streak virus. J Gen Virol 70: 2309–2323

    Google Scholar 

  4. Briddon RW, Pinner MS, Stanley J, Markham PG (1990) Geminivirus coat protein gene replacement alters insect specificity. Virology 177: 85–94

    Google Scholar 

  5. Briddon RW, Lunness P, Chamberlin LCL, Pinner MS, Brundish H, Markham PG (1992) The nucleotide sequence of an infectious insect-transmissible clone of the geminivirus panicum streak virus. J Gen Virol 73: 1041–1047

    Google Scholar 

  6. Chatani M, Matsumoto Y, Mizuta H, Ikegami M, Boulton MI, Davies JW (1991) The nucleotide sequence and genome structure of the geminivirus miscanthus streak virus. J Gen Virol 72: 2325–2331

    Google Scholar 

  7. Dekker EL, Pinner MS, Markham PG, Van Regenmortel MHV (1988) Characterisation of maize streak virus isolates from different plant species by polyclonal and monoclonal antibodies. J Gen Virol 69: 983–990

    Google Scholar 

  8. Devereux J, Haeberli P, Smithies O (1984) A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res 12: 387–395

    Google Scholar 

  9. Donson J, Accotto G-P, Boulton MI, Mullineaux PM, Davies JW (1987) The nucleotide sequence of a geminivirus fromDigitaria sanguinalis. Virology 161: 160–169

    Google Scholar 

  10. Donson J, Morris-Krsinich BAM, Mullineaux PM, Boulton MI, Davies JW (1984) A putative primer for second-strand DNA synthesis of maize streak virus is virion-associated. EMBO J 3: 3069–3073

    Google Scholar 

  11. Fenoll C, Black DM, Howell SH (1988) The intergenic region of maize streak virus contains promoter elements involved in rightward transcription of the viral genome. EMBO J 1589–1596

  12. Fenoll C, Schwarz JJ, Black DM, Schneider M, Howell SH (1990) The intergenic region of maize streak virus contains a GC-rich element that activates rightward transcription and binds maize nuclear factors. Plant Mol Biol 15: 865–877

    Google Scholar 

  13. Gibbs A, Fenner F (1984) Methods for comparing sequence data such as restriction endonuclease maps or nucleotide sequences of viral nucleic acid molecules. J Virol Methods 9: 317–324

    Google Scholar 

  14. Hayes RJ, Macdonald H, Coutts RHA, Buck KW (1988) Priming of complementary DNA synthesisin vitro by small DNA molecules tightly bound to virion DNA of wheat dwarf virus. J Gen Virol 69: 1345–1350

    Google Scholar 

  15. Henikoff S (1984) Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene 28: 351–359

    Google Scholar 

  16. Higgins DG, Bleasby AJ, Fuchs R (1992) CLUSTAL-V-Improved software for multiple sequence alignment. Comput Appl Biosci 8: 189–191

    Google Scholar 

  17. Howarth AJ, Vandemark GJ (1989) Phylogeny of geminiviruses. J Gen Virol 70: 2717–2727

    Google Scholar 

  18. Howell SH (1985) Physical structure and genetic organisation of the genome of maize streak virus (Kenyan isolate). Nucleic Acids Res 13: 3018–3019

    Google Scholar 

  19. Hughes FL, Rybicki EP, Kirby R, von Wechmar MB (1991) Characterization of the sugarcane streak agent as a distinct geminivirus. Intervirology 32: 19–27

    Google Scholar 

  20. Hughes FL, Rybicki EP, von Wechmar MB (1992) Genome typing of southern African subgroup 1 geminiviruses. J Gen Virol 73: 1031–1040

    Google Scholar 

  21. Hull R, Stanley J, Briddon RW (1991) Geminivirus. In: Francki RIB, Fauquet CM, Knudson DL, Brown F (eds) Classification and nomenclature of viruses: Fifth Report of the International Committee on Taxonomy of Viruses. Springer, Wien New York, pp 173–177. (Arch Virol [Suppl 2])

    Google Scholar 

  22. Lazarowitz SG (1987) The molecular characterization of geminiviruses. Plant Mol Biol Reptr 4: 177–192

    Google Scholar 

  23. Lazarowitz SG (1988) Infectivity and complete nucleotide sequence of the genome of a South African isolate of maize streak virus. Nucleic Acids Res 16: 229–249

    Google Scholar 

  24. Lazarowitz SG, Pinder AJ, Damsteegt VD, Rogers SG (1989) Maize streak virus genes essential for systemic spread and symptom development. EMBO J 8: 1023–1032

    Google Scholar 

  25. MacDowell SW, Macdonald H, Hamilton WDO, Coutts RHA, Buck KW (1985) The nucleotide sequence of cloned wheat dwarf virus DNA. EMBO J 4: 2173–2180

    Google Scholar 

  26. McClean APD (1947) Some forms of streak virus occuring in maize, sugarcane and wild grasses. SA Dept Agric Sci Bull 265: 33 pp

    Google Scholar 

  27. Messing J, Geraghty D, Heidecker G, Hu N, Kridl J, Rubinstein I (1983) Plant gene structure. In: Kasuge T, Meredith CP, Hollaender A (eds) Genetic engineering of plants. Plenum Press, New York, pp 211–227

    Google Scholar 

  28. Morris BAM, Richardson KA, Haley A, Zhan XC, Thomas JE (1992) The nucleotide sequence of the infectious cloned DNA component of tobacco yellow dwarf virus reveals features of geminiviruses infecting monocotyledonous plants. Virology 187: 633–642

    Google Scholar 

  29. Morris-Krsinich BAM, Mullineaux PM, Donson J, Boulton MI, Markham PG, Short MN, Davies JW (1985) Bidirectional transcription of maize streak virus DNA and identification of the coat protein gene. Nucleic Acids Res 13: 7237–7256

    Google Scholar 

  30. Mullineaux PM, Donson J, Morris-Krsinich BAM, Boulton MI, Davies JW (1984) The nucleotide sequence of maize streak virus DNA. EMBO J 3: 3063–3068

    Google Scholar 

  31. Mullineaux PM, Guerineau F, Accotto GP (1990) Processing of complementary sense RNAs of digitaria streak virus in its host and in transgenic tobacco. Nucleic Acids Res 18: 7259–7265

    Google Scholar 

  32. Pinner MS, Markham PG (1990) Serotyping and strain identification of maize streak virus isolates. J Gen Virol 71: 1635–1640

    Google Scholar 

  33. Pinner MS, Markham PG, Markham RH, Dekker EL (1988) Characterisation of maize streak virus: description of strains, symptoms. Plant Pathol 37: 74–87

    Google Scholar 

  34. Pinner MS, Rybicki EP, Greber RS, Markham PG (1992) Serological relationships of geminivirus isolates from Gramineae in Australia. Plant Pathol 41: 618–625

    Google Scholar 

  35. Rybicki EP (1991) The use of serological differentiation indices for the phylogenetic analysis of plant virus relationships. Arch Virol 119: 83–93

    Google Scholar 

  36. Rybicki EP, Hughes FL (1990) Detection and typing of maize streak virus and other distantly related geminiviruses of grasses by polymerase chain reaction amplification of a conserved viral sequence. J Gen Virol 71: 2519–2526

    Google Scholar 

  37. Saitou N, Nei M (1987) The neighbour-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4: 406–425

    Google Scholar 

  38. Salser W (1977) Globin mRNA sequences: analysis of base pairing and evolutionary implications. Cold Spring Harb Symp Quant Biol 42: 985–1002

    Google Scholar 

  39. Sanger F, Miklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467

    Google Scholar 

  40. Schalk H-J, Matzeit V, Schiller B, Schell J, Gronenborn B (1989) Wheat dwarf virus, a geminivirus of graminaceous plants needs splicing for replication. EMBO J 8: 359–364

    Google Scholar 

  41. Schneider M, Jarchow E, Hohn B (1992) Mutational analysis of the conserved region of maize streak virus suggests its involvement in replication. Plant Mol Biol: 1–10

  42. Studier JA, Keppler KL (1988) A note on the neighbour-joining algorithm of Saitou and Nei. Mol Biol Evol 5: 729–731

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Microbiology, University of Cape Town, Rondebosch

    F. L. Hughes & E. P. Rybicki

  2. Department of Microbiology, Rhodes University, Grahamstown, South Africa

    R. Kirby

Authors
  1. F. L. Hughes
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. P. Rybicki
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R. Kirby
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hughes, F.L., Rybicki, E.P. & Kirby, R. Complete nucleotide sequence of sugarcane streak Monogeminivirus. Archives of Virology 132, 171–182 (1993). https://doi.org/10.1007/BF01309851

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01309851

Keywords

Search

Navigation