Skip to main content
SpringerLink
Log in

The stimulating influence of the raphé nuclei on the morphofunctional development of the hippocampus during their combined cultivation

  • Published:
Neuroscience and Behavioral Physiology Aims and scope Submit manuscript

Abstract

It has been demonstrated in combined (raphé nuclei-hippocampus, cerebellum-hippocampus) organotypical cultures of the brain of newborn rats that, unlike cerebellar expiants, the raphé nuclei grow in an oriented manner toward the hippocampus and exert a stimulating influence on its morphofunctional development; this is manifested: a) in an intense proliferation and migration of glial cells, which are accompanied by accelerated growth of neuronal processes and an increase in the dimensions of the initial piece; b) in the earlier formation of the background electrical activity of the neurons; c) in an increase in these cultures in the number of active cells; d) in a significant predominance of neurons with periodic (in the form of trains or groups) discharges. These data are in agreement with previously established effects of exogenous serotonin, and attest to an important role of the serotoninergic influences of the raphé nuclei in the morphofunctional development of the hippocampus.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. I. V. Viktorov, “The participation of neuroglia in the formation of connections between isolated brain explants,” in:The Functions of the Neuroglia [in Russian], Tbilisi (1979), pp. 154–160.

  2. M. D. Gedevanishvili, L. E. Tsutsunava, and N. A. Malazoniya, “Serotonin independently stimulates the adhesion and multiplication of fibroblasts in culture,”Tsitologiya,24, No. 2, 224–226 (1982).

    Google Scholar 

  3. J. Paul,The Culture of Cells and Tissues [Russian translation], Moscow (1963).

  4. A. R. Chubakov and O. S. Sotnikov, “The formation of connections between the nuclei of the raphé and hippocampus in tissue culture (intravital functional-morphological investigations),”Arkh. Anatomii, Gistologii i Émbriologii,82, No. 3, 11–20 (1982).

    Google Scholar 

  5. A. R. Chubakov, É. F. Sarkisova, and V. G. Tsyganova, “Serotonin and the functional development of the hippocampus in tissue culture,”Ontogenez,14, No. 5, 518–524 (1983).

    PubMed  Google Scholar 

  6. E. I. Chumasov, A. R. Chybakov, G. V. Konovalov, and E. A. Gromova, “Influence of serotonin on the growth and differentiation of cellular elements of the hippocampus in culture conditions,”Arkh. Anatomii, Gistologii i Émbriologii,74, No. 1, 98–106 (1978).

    Google Scholar 

  7. E. I. Chumasov, A. R. Chubakov, and V. G. Tsyganova, “The relationship of the differentiation of neurons of the cerebral cortex and hippocampus to the development of neuroglial elements,” in:The Ultrastructure of Neurons and Pharmacological Actions [in Russian], Pushchino (1981), pp. 154–160.

  8. B. H. Choi and L. W. Lapham, “Interactions of neurons and astrocytes during growth and development of human fetal brain in vitro,”Exp. Mol. Pathol.,24, No. 1, 110–125 (1976).

    PubMed  Google Scholar 

  9. T. Ebendol and C. O. Jacobson, “Tissue explants affecting extension and orientation of axons in cultured chick embryo ganglia,”Exper. Cell Res.,105, No. 2, 379–387 (1977).

    Google Scholar 

  10. Z. Lodin, P. Korinkova, J. Faltin, and V. Fleischmannova, “Differentiation of corpus callosum glial cells and factors influencing their maturation in vitro,”Acta Histochem.,61, No. 2, 184–191 (1978).

    PubMed  Google Scholar 

  11. M. Segal, “Physiological and pharmacological evidence for a serotoninergic projection to the hippocampus,”Brain Res.,94, No. 1, 115–131 (1975).

    PubMed  Google Scholar 

  12. M. Yamamoto, H. W. M. Steinbusch, and T. M. Jessell, “Differentiated properties of identified serotonin neurons in dissociated cultures of embryonic rat brain stem,”J. Cell Biology,91, No. 1, 142–152 (1981).

    Google Scholar 

  13. K. Yoshida, S. Kohsaka, T. Nii, et al., “Subcultured astrocytes suppress the proliferation of neuroblasts in vitro,”Neurosci Lett.,70, No. 1, 34–39 (1986).

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino

    A. R. Chubakov, V. G. Tsyganova & É. F. Sarkisova

Authors
  1. A. R. Chubakov
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. V. G. Tsyganova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. É. F. Sarkisova
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Translated from Fiziologicheskii Zhurnal imeni I. M. Sechenova, Vol. 78, No. 2, pp. 55–61, February, 1992.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chubakov, A.R., Tsyganova, V.G. & Sarkisova, É.F. The stimulating influence of the raphé nuclei on the morphofunctional development of the hippocampus during their combined cultivation. Neurosci Behav Physiol 23, 271–276 (1993). https://doi.org/10.1007/BF01182928

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01182928

Keywords

Search

Navigation