Skip to main content
SpringerLink
Log in

Arachidonic acid-induced swelling in incubated rat brain cortical slices

Effect of bovine serum albumin

  • Original Articles
  • Published:
Neurochemical Research Aims and scope Submit manuscript

Abstract

The influence of bovine serum albumin (BSA) on the rat brain cortical swelling induced by sodium arachidonate and polyunsaturated fatty acids has been studied. Coincubation of arachidonate with BSA at a molar ratio of 5 (arachidonate/BSA) or less greatly inhibited the arachidonate-induced swelling. As the molar ratio of arachidonate/BSA increased, the degree of swelling increased. The swelling was not reversed by BSA, although the BSA released 46% of the previously incorporated [3H]arachidonic acid from the cortical slices. The entry of [3H]arachidonate into the slice was completely abolished by 0.1 mM BSA or partially inhibited by exogenous arachidonate. It is concluded that the induction of brain swelling by arachidonate requires the intracellular transport of exogenous arachidonate.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Chan, P. H., andFishman, R. A. 1978. Brain edema: Induction in cortical slices by polyunsaturated fatty acids. Science 201:358–360.

    Google Scholar 

  2. Kimelberg, H. K., andPapahadjopoulos, D. 1971. Phospholipid-protein interactions: Membrane permeability correlated with monolayer “penetration.” Biochim. Biophys. Acta 233:805–809.

    Google Scholar 

  3. Solomonson, L. P., Leipkalns, V. A., andSpector, A. A. 1976. Changes in (Na++K+)-ATPase activity of Ehrlich ascites tumor cells produced by alteration of membrane fatty acid composition. Biochemistry 15:892–897.

    Google Scholar 

  4. Shafrir, E., Gatt, S., andKhasis, S. 1965. Partition of fatty acids of 20–24 carbon atoms between serum albumin and lipoproteins. Biochim. Biophys. Acta 98:365–371.

    Google Scholar 

  5. Havel, R. M., Ekelund, L.-G., andHolmgren, A. 1967. Kinetic analysis of the oxidation of palmitate-1-14C in man during prolonged heavy muscular exercise. J. Lipid Res. 8:366–373.

    Google Scholar 

  6. Saifer, A., andGoldman, L. 1961. The free fatty acids bound to human serum albumin. J. Lipid Res. 2:268–270.

    Google Scholar 

  7. Spector, A. A. 1968. The transport and utilization of free fatty acid. Ann. N. Y. Acad. Sci. 149:768–783.

    Google Scholar 

  8. Spector, A. A. 1971. Metabolism of free fatty acids. Prog. Biochem. Pharmacol. 6:130–176.

    Google Scholar 

  9. Bourke, R. S., andTower, D. B. 1966. Fluid compartmentation and electrolytes of cat cerebral cortex in vitro. II. Sodium, potassium and chloride of mature cerebral cortex. J. Neurochem. 13:1099–1117.

    Google Scholar 

  10. Chan, P. H., Wong, Y. P., andFishman, R. A. 1978. Hyperosmolality-induced GABA release from rat brain slices: Studies of calcium dependency and sources of release. J. Neurochem. 30:1363, 1368.

    Google Scholar 

  11. Fishman, R. A., Reiner, M., andChan, P. H. 1977. Metabolic changes associated with iso-osmotic regulation in brain cortex slices. J. Neurochem. 28:1061–1067.

    Google Scholar 

  12. Isakson, P. C., Raz, A., andNeedleman, P. 1976. Effective incorporation of14C-arachidonic acid into the phospholipids of intact tissues and subsequent metabolism to14C-prostaglandins. Prostaglandins 12:739–748.

    Google Scholar 

  13. Fishman, R. A., Sligar, K., andHake, R. B. 1977. Effects of leukocytes on brain metabolism in granulocytic brain edema. Ann. Neurol. 2:89–94.

    Google Scholar 

  14. Spector, A. A., Steinberg, D., andTanaka, A. 1965. Uptake of free fatty acids by Ehrlich ascites tumor cells. J. Biol. Chem. 240:1032–1041.

    Google Scholar 

  15. Kimelberg, H. K., andPapahadjopoulos, D. 1974. Effects of phospholipid acyl chain fluidity, phase transition and cholesterol on (Na++K+)-stimulated adenosine triphosphatase. J. Biol. Chem. 249:1071–1080.

    Google Scholar 

  16. Kesner, L., Kindya, R. J., andChan, P. C. 1979. Inhibition of erythrocyte membrane (Na++K+)-activated ATPase by ozone-treated phospholipids. J. Biol. Chem. 254:2705–2709.

    Google Scholar 

  17. Fishman, R. A., Chan, P. H., Lee, J., andQuan, S. 1979. Effects of superoxide free radicals on the induction of brain edema. Neurology 29:546.

    Google Scholar 

  18. Hexum, T. D., andFried, R. 1979. Effects of superoxide radicals on transport (Na+K) adenosine triphoshatase and protection by superoxide dismutase. Neurochem. Res. 4:73–82.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Brdin Edema Clinical Research Center Department of Neurology, University of California, 94143, San Francisco, California

    Pak H. Chan, Robert A. Fishman, Janie L. Lee & Susan C. Quan

Authors
  1. Pak H. Chan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Robert A. Fishman
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Janie L. Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Susan C. Quan
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chan, P.H., Fishman, R.A., Lee, J.L. et al. Arachidonic acid-induced swelling in incubated rat brain cortical slices. Neurochem Res 5, 629–640 (1980). https://doi.org/10.1007/BF00964784

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00964784

Keywords

Search

Navigation