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Extrahepatic expression of plasma protein genes during inflammation

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Abstract

The body's protective responses to infection, wounding, trauma, and malignancy include the acute-phase reaction, which is modulated by various cytokines and their cellular receptors. During the acute-phase reaction, levels of specific proteins synthesized by the liver increase in the plasma. Little information is available about the extrahepatic synthesis of plasma proteins during the acute-phase reaction. The study described here analyzes the tissue-specific expression of genes encoding the plasma proteins albumin (ALB), α1,-antitrypsin (AAT), transferrin (TF), haptoglobin (HP), ceruloplasmin (CP), serum amyloid A (SAA), α1-acid glycoprotein (AGP) and α2-HS-glycoprotein (AHSG) during the acute-phase reaction in C57B1 mice. The acute-phase reaction was induced by intraperitoneal injections of bacterial lipopolysaccharide (LPS). During the acute-phase reaction, genes encoding CP, SAA, AGP, and HP demonstrate unique extrahepatic tissue specific patterns of expression in kidney, spleen, thymus, heart, brain, lung, testis, and epididymis. Different temporal patterns of HP gene expression also were observed in lung and thymus after induction by LPS. The function of extrahepatic synthesis of plasma proteins is not yet understood; however, a local provision of specific plasma proteins in mammalian tissues may offer the host a source of functionally important proteins during periods of stress.

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References

  1. Kushner, I., andA. Mackiewicz. 1987. Acute phase proteins as disease markers.Dis. Markers 5:1–11.

    Google Scholar 

  2. Adrian, G. S., D. C.Herbert, L. K.Robinson, C. A.Walter, J. M.Buchanan, E. K.Adrian, F. J.Weaker, C. A.Eddy, F.Yang, and B. H.Bowman. 1991. Expression of a human chimeric transferrin gene in senescent transgenic mice reflects the decrease of transferrin levels in aging humans (submitted).

  3. Pepys, M. B., M. D. Baltz, K. Gomer, A. S. Davies, andM. Doenhoff. 1979. Serum amyloid P-component is an acute phase reactant in the mouse.Nature 278:259–261.

    Google Scholar 

  4. Lum, J. B., A. J. Infante, D. M. Marker, F. Yang, andB. H. Bowman. 1986. Transferrin synthesis by inducer T lymphocytes.J. Clin. Invest. 77:841–849.

    Google Scholar 

  5. Levin, M. J., D. Tuil, G. Uzan, J.-C. Dreyfus, andA. Kahn. 1984. Expression of the transferrin gene during development of nonhepatic tissues: High level of transferrin mRNA in fetal muscle and adult brain.Biochem. Biophys. Res. Commun. 122:212–217.

    Google Scholar 

  6. Aldred, A. R., A. Grimes, G. Schreiber, andJ. F. B. Mercer. 1987. Rat ceruloplasmin. Molcular cloning and gene expression in liver, choroid plexus, yolk sac, placenta and testis.J. Biol. Chem. 262:2875–2878.

    Google Scholar 

  7. Skinner, M. K., andM. D. Griswold. 1983. Sertoli cells synthesize and secrete a ceruloplasmin-like protein.Biol. Reprod. 28:1225–1229.

    Google Scholar 

  8. Dickson, P. W., A. R. Aldred, P. D. Marley, G. F. Tu, G. J. Howlett, andG. Schreiber. 1985. High prealbumin and transferrin mRNA levels in the choroid plexus of rat brain.Biochem. Biophys. Res. Commun. 127:890–895.

    Google Scholar 

  9. Ruther, U., M. Tripodi, R. Cortese, andE. F. Wagner. 1987. The human alpha-1-antitrypsin gene is efficiently expressed from two tissue specific promoters in transgenic mice.Nucleic Acids Res. 15:7519–7529.

    Google Scholar 

  10. Kelsey, G. D., S. Povey, A. E. Bygrave, andR. H. Lovell-Badge. 1987. Species and tissue-specific expression of human alpha-1-antitrypsin in transgenic mice.Genes Dev. 1:161–171.

    Google Scholar 

  11. Koopman, P., S. Povey, andR. H. Lovell-Badge. 1989. Widespread expression of humanα 1-antitrypsin in transgenic mice revealed by in situ hybridization.Genes Dev. 3:16–25.

    Google Scholar 

  12. Schreiber, G., A. Tsykin, A. R. Aldred, T. Thomas, Wai-Ping Fung, P. W. Dickson, T. Cole, H. Birch, F. A. De Jong, andJ. Milland. 1989. The acute phase response in the rodent.Ann. N.Y. Acad. Sci. 557:61–86.

    Google Scholar 

  13. Meek, R. L., andE. P. Benditt. 1986. Amyloid A gene family expression in different mouse tissues.J. Exp. Med. 164:2006–2017.

    Google Scholar 

  14. Katz, Y., M. Revel, andR. C. Struck. 1989. Interleukin 6 stimulates synthesis of complement proteins factor B and C3 in human skin fibroblasts.Eur. J. Immunol. 19:983–988.

    Google Scholar 

  15. Chomczynski, P., andN. Sacchi. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.Anal. Biochem. 162:156–159.

    Google Scholar 

  16. Fourney, R. M., J. Miyakoshi, R. S. Day, III andM. C. Paterson. 1988. Northern blotting: Efficient RNA staining and transfer.Focus (Bethesda Res. Lab.) 10:5–7.

    Google Scholar 

  17. Leharch, H., D. Diamond, J. M. Wozney, andH. Boedtker. 1977. RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination.Biochemistry 16:4743–4751.

    Google Scholar 

  18. Davies, L. G., M. D. Dibner, andJ. F. Battey. 1986. InBasic Methods in Molecular Biology. Elsevier Science Publishing, New York, 143–149.

    Google Scholar 

  19. Feinberg, A. P., andB. Vogelstein. 1983. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.Anal. Biochem. 132:6–13.

    Google Scholar 

  20. Yang, F., J. L. Brune, W. D. Baldwin, D. R. Barnett, andB. H. Bowman. 1983. Identification and characterization of human haptoglobin cDNA.Proc. Natl. Acad. Sci. U.S.A. 80:5875–5879.

    Google Scholar 

  21. Yang, F., J. B. Lum, J. R. McGill, C. M. Moore, S. L. Naylor, P. H. Van Bragt, W. D. Baldwin, andB. H. Bowman. 1984. Human transferrin: cDNA characterization and chromosomal localization.Proc. Natl. Acad. Sci. U.S.A. 81:2752–2756.

    Google Scholar 

  22. Yang, F., S. L. Naylor, J. B. Lum, S. Cutshaw, J. L. McCombs, K. H. Naberhaus, J. R. McGill, G. S. Adrian, C. M. Moore, D. R. Barnett, andB. H. Bowman. 1986. Characterization, mapping and expression of human ceruloplasmin gene.Proc. Natl. Acad. Sci. U.S.A. 83:3257–3261.

    Google Scholar 

  23. Yang, F., Z.-L.Chen, J. M.Bergeron, R. L.Cupples, and W. E.Friedrichs. 1991. Human alpha-2-HS-gylcoprotein/bovine fetuin homologue in mice: Identification of the cDNA sequence and developmertal regulation of the gene (submitted).

  24. Yamamoto, K.-I., andS. Migita. 1985. Complete primary structures of two major murine serum amyloid A proteins deduced from cDNA sequences.Proc. Natl. Acad. Sci. U.S.A. 82:2915–2919.

    Google Scholar 

  25. Lee, S.-C., C.-J. Chang, Y.-M. Lee, H.-Y. Lei, M.-Y. Lai, andD.-S. Chen. 1989. Molecular cloning of cDNAs corresponding to two genes of alpha-1-acid glycoprotein and characterization of two alleles of alpha-1-acid glycoprotein in the mouse.DNA 8(4):245–251.

    Google Scholar 

  26. Sifers, R. N., F. D. Ledley, L. Reed-Fourquet, D. H. Ledbetter, S. A. Ledbetter, andS. L. C. Woo. 1990. Complete cDNA sequence and chromosomal localization of mouse alpha1-antitrypsin.Genomic 6:100–104.

    Google Scholar 

  27. Draper, N. R., andH. Smith. 1966. Applied Regression Analysis. John Wiley & Sons, New York. 77–81.

    Google Scholar 

  28. Yang, F., W. E. Friedrichs, J. M. Buchanan, D. C. Herbert, F. J. Weaker, J. H. Brock, andB. H. Bowman. 1990. Tissue specific expression of mouse transferrin during development and aging.Mech. Age. Dev. 56:187–197.

    Google Scholar 

  29. Lang, C. H., G. J. Bagby, andJ. J. Spitzer. 1985. Glucose kinetics and body temperature after lethal and nonlethal doses of endotoxin.Ann. J. Physiol. 248:R471-R478.

    Google Scholar 

  30. Filkins, J. P. andN. R. Diluzio. 1968. Endotoxin induced hypothermia and tolerance in the rat.Proc. Soc. Exp. Biol Med. 129:724–726.

    Google Scholar 

  31. Inarrea, P., J. Cambronero-Gonez, J. Pascual, M. Del C. Ponte, L. Herando, andM. Sanchez-Crespo. 1985. Synthesis of PAF-acether and blood volume changes in gram-negative sepsis.Immunopharmacology 9:45–52.

    Google Scholar 

  32. Baumhueter, S., D. B. Mendel, P. B. Conley, C. J. Kuo, C. Turk, M. K. Graves, C. A. Edwards, G. Courtois, andG. R. Crabtree. 1990. HNF-1 shares three sequence motifs with the POU domain proteins and is identical to LB-B1 and APF.Genes Dev. 4:372–379.

    Google Scholar 

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Author notes

  1. Nuanthip Kalmovarin

    Present address: Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, 10330, Bangkok, Thailand

Authors and Affiliations

  1. Department of Cellular and Structural Biology, University of Texas Health Science Center, 78284-7762, San Antonio, Texas

    Nuanthip Kalmovarin, William E. Friedrichs, Houston V. O'brien, Leslie A. Linehan, Barbara H. Bowman & Funmei Yang

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  1. Nuanthip Kalmovarin
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  2. William E. Friedrichs
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  3. Houston V. O'brien
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  4. Leslie A. Linehan
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Additional information

Supported in part by NIH grants GM33298, AG0672, and AG06650 and a fellowship to N.K. from the Educational Commission for Foreign Medical Graduates, Philadelphia.

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Kalmovarin, N., Friedrichs, W.E., O'brien, H.V. et al. Extrahepatic expression of plasma protein genes during inflammation. Inflammation 15, 369–379 (1991). https://doi.org/10.1007/BF00917353

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