Abstract
Several types of polymeric glycoconjugates,N-substituted polyacrylamides, have been synthesized by the reaction of activated polymers with ω-aminoalkylglycosides: (i) (carbohydrate-spacer) n -polyacrylamide, ‘pseudopolysaccharides’; (ii) (carbohydrate-spacer) n -phosphatidylethanolamine m -polyacrylamide, neoglycolipids, derivatives of phosphatidylethanolamine; (iii) (carbohydrate-spacer) n -biotin m -polyacrylamide, biotinylated probes; (iv) (carbohydrate-spacer) n -polyacrylamide-(macroporous glass), affinity sorbents based on macroporous glass, covalently coated with polyacrylamide. An almost quantitative yield in the conjugation reaction makes it possible to insert in the conjugate a predetermined quantity of the ligand(s).
Pseudopolysaccharides proved to be a suitable form of antigen for activation of polystyrene and poly(vinyl chloride) plates (ELISA) and nitrocellulose membranes (dot blot), being advantageous over traditional neoglycoproteins. Polyvalent glycolipids insert well in biological membranes: their physical properties, particularly solubility, can be changed in a desired direction. Biotinylated derivatives were used as probes for detection and analysis of lectins.
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Abbreviations
- sp:
-
spacer arm
- PAA:
-
polyacrylamide
- PE:
-
phosphatidylethanolamine
- Biot:
-
biotin
- MPGlass:
-
macroporous glass 200 Å
- ELISA:
-
enzyme-linked immunosorbent assay
- HPLC:
-
high-performance liquid chromatography
- AIBN:
-
azodiisobutyronitrile
- BSA:
-
bovine serum albumin
- DMG:
-
3,6-di-O-methyl-d-glucose
- TLC:
-
thin-layer chromatography
- DMF:
-
dimethylformamide
- DMSO:
-
dimethylsulfoxide
- RI:
-
refractive index
- PBS:
-
phosphate buffered saline (0.14m NaCl, 0.01m sodium phosphate, pH 7.3)
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Bovin, N.V., Korchagina, E.Y., Zemlyanukhina, T.V. et al. Synthesis of polymeric neoglycoconjugates based onN-substituted polyacrylamides. Glycoconjugate J 10, 142–151 (1993). https://doi.org/10.1007/BF00737711
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DOI: https://doi.org/10.1007/BF00737711