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Immunohistological evidences of cortical and medullary differentiation in thymoma

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Summary

The phenotypical characteristics of human epithelial and lymphoid cells have been studied with immunohistochemical methods on frozen sections of 12 thymomas. On the basis of the cytohistological characteristics of thymoma epithelial cells (EC) the thymomas were divided in cortical, medullary and mixed types, according to recently developed light microscopical criteria. When tested with a series of monoclonal antibodies, thymoma EC were all stained by the antibody Ki-M3 (as in the thymus), but reacted with anti-HLA-DR, anti-HLA-A,B,C and with a new monoclonal antibody to cortical EC,21A6, to a lesser extent and with weaker, variable intensity in comparison with the normal thymus. Cortical type thymomas were most reactive and the medullary type almost negative. Thymomas, like normal thymus showed different immunoreactivity patterns with antibodies to prekeratins of different specificities. Cortical type thymomas and areas in mixed thymoma showed an EC staining with the antibody to non-squamous type keratin (35βH11) whereas medullary type thymomas and areas showed staining with antibodies to squamous-type keratin (34βE12-IV/82) in addition. Lymphoidcellswithcortical(OKT6+,Leu 1 weakly+,Leu2a+,Leu3a+) or mature medullary (OKT6-, Leu 1 strongly+, Leu 2a or Leu 3a+) phenotype were found to colonize tumours with diferent EC types. These immunohistochemical findings largely confirm our earlier cytological distinction of thymoma EC. In addition important differences have been observed in neoplastic cortical EC concerning the HLA-DR and 21A6 immunoreactivity that may be intimately related to the neoplastic process and paraneoplastic immune phenomena.

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References

  • Castleman B (1955) Tumours of the thymus gland. In:Atlas of Tumor Pathology, Fascicle 19. Armed Forces Institute of Pathology, Washington

    Google Scholar 

  • Chan WC, Zaatari GS, Tabei S, Bibb M, Brynes RK (1984) Thymoma: an immunohistochemical study. Am J Clin Pathol 82:160–166

    Google Scholar 

  • Chilosi M, Menestrina F, Iannucci AM, Fiore-Donati L, Pizzolo G, Janossy G (1984) Immunohistochemical analysis of thymoma. Evidence for medullary origin of epithelial cells. Am J Surg Pathol 8:309–318

    Google Scholar 

  • McFarland EJ, Scearce RM, Haynes BF (1984) The human thymic microenvironemnt:cortical thymic epithelium is an antigenically distinct region of the thymic microenvironment. J Immunol 133:1241–1249

    Google Scholar 

  • Gaudecker B von (1985) The development of human thymus microenvironment. In:Müller-Hermelink HK (ed) Histophysiology and pathology of the human thymus. Current Topics in Pathology, vol. 75, Springer Verlag, Berlin Heidelberg New York, in press

    Google Scholar 

  • Graham RC, Karnovsky MJ (1966) The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney. Ultrastructural cytochemistry by a new technique. J Histochem Cytochem 14:219–302

    Google Scholar 

  • Gray GF, Gutowski WT III (1979) Thymoma. A clinicopathologic study of 54 cases. Am J Surg Pathol 3:235–249

    Google Scholar 

  • Haynes BF (1984) The human thymic microenvironment. In:Dixon FJ (ed) Advances in Immunology, vol 36. Academic Press Inc., Orlando San Diego New York, pp 87–142

    Google Scholar 

  • Haynes BF, Robert-Guroff M, Metzgar RS, Franchini G, Kalyanaraman VS, Palker TJ, Gallo RC (1983) Monoclonal antibody against human T-cell leukemia virus p19 definies a human thymic epithelial antigen acquired during ontogeny. J Exp Med 157:907–920

    Google Scholar 

  • Haynes BF, Scearce RM, Lobach DF, Hensley LL (1984) Phenotypic characterization and ontogeny of mesodermal-derived and endocrine epithelial components of the human thymic microenvironment. J Exp Med 159:1149–1168

    Google Scholar 

  • Hofmann W, Möller P, Momburg F, Moldenhauer G, Otto HF (1984) Struktur des normalen Thymus, der lymphofollikulären Thymushyperplasie und der Thymome, dargestellt mit Lectinen, S-100-Protein- und Keratin Antiseren, und monoklonalen (epitheliotropen) Antikörpern. Verh Dtsch Ges Path 68:504

    Google Scholar 

  • Hofmann W, Möller P, Manke H-G, Otto HF (1985) Thymoma. A clinicopathologic study of 98 cases with special reference to three unusual cases. Path Res Pract 179:337–353

    Google Scholar 

  • Janossy G, Bofill M, Trejdosiewicz LK, Poulter LW, Chilosi M, Newson-Davis J (1985) Cellular differentiation of lymphoid subpopulations and their microenvironment in the human thymus. In:Müller-Hermelink HK (ed) Histophysiology and pathology of the human Thymus. Current Topics in Pathology, vol. 75, Springer Verlag, Berlin Heidelberg New York, in press

    Google Scholar 

  • Kuroda Y, Oda K-I, Neshige R, Shibasaki H (1984) Exacerbation of myasthenia gravis after removal of a thymoma having a membrane phenotype of suppressor T cells. Ann Neurol 15:400–402

    Google Scholar 

  • Lattes R (1962) Thymoma and other tumors of the thymus. An analysis of 107 cases. Cancer 15:1224–1260

    Google Scholar 

  • Lauriola L, Maggiano N, Marino M, Carbone A, Piantelli M, Musiani P (1981) Human thymoma: immunologic characteristics of the lymphocytic component. Cancer 48:1992–1995

    Google Scholar 

  • Lauriola L, Musiani P, Ranelletti FO, Maggiano N, Piantelli M (1983) Human thymoma lymphocyte mytogenesis: glucocorticoid inhibitory capacity as a function of the size of the more mature T cells subset. Clin Exp Immunol 52:477–484

    Google Scholar 

  • Levine GD, Bensch KG (1972) Epithelial nature of spindle-cell thymoma. An ultrastructural study. Cancer 30:500–511

    Google Scholar 

  • Marino M, Müller-Hermelink HK (1985) Thymoma and thymic carcinoma. Relation of thymoma epithelial cells to the cortical and medullary differentiation of thymus. Virchows Arch [Pathol Anat] 407:119–149

    Google Scholar 

  • Mokhtar N, Hsu S-M, Rajnikant P, Haynes BF, Jaffe ES (1984) Thymoma: lymphoid and epithelial components mirror the phenotype of normal thymus. Hum Pathol 15:378–384

    Google Scholar 

  • Müller-Hermelink HK, Marino M (1984) Zytologische Befunde und Malignitatskriterien menschlicher Thymome. Verh Dtsch Ges Path 68:454

    Google Scholar 

  • Müller-Hermelink HK, Steinmann GG (1984) Age-related alternations of intrathymic microenvironments. In: De Week AL (ed) Lymphoid cell functions in aging. Topics in aging research in Europe, vol 3. Rijswijk Eurage, pp 75–82

    Google Scholar 

  • Müller-Hermelink HK, Marino M, Palestro G (1985) Pathology of thymic epithelial tumors. In:Müller-Hermelink HK (ed) Histophysiology and pathology of the human thymus. Current Topics in Pathology, vol. 75, Springer Verlag, Berlin Heidelberg New York, in press

    Google Scholar 

  • Musiani P, Lauriola L, Maggiano N, Tonali P, Piantelli M (1982) Functional properties of human thymoma lymphocytes: role of subcellular factors in blastic activation. J Natl Cancer Inst 69:827–831

    Google Scholar 

  • Reddick RL, Jennette JC (1983) Immunologic and ultrastructural characterization of the small cell population in malignant thymoma. Hum Pathol 14:377–380

    Google Scholar 

  • Ritter MA, Sauvage CA, Cotmore SF (1981) The human thymus microenvironment: in vivo identification of thymic nurse cells and other antigenically distinct subpopulations of epithelial cells. Immunology 44:439–446

    Google Scholar 

  • Rosai J, Levine GD (1976) Tumors of the thymus. In:Atlas of Tumor Pathology. 2nd Series, Fascicle 13. Armes Forces Institute of Pathology, Washington

    Google Scholar 

  • Savino W, Berrih S, Dardenne M (1984) Thymic epithelial antigen, acquired during ontogeny and definied by the anti-p19 monoclonal antibody, is lost in thymomas. Lab Invest 51:292–296

    Google Scholar 

  • Sternberger LA, Hardy PH jr. Cuculis JJ, Meyer HG (1970) The unlabeled antibody enzyme method of immunohistochemistry. Preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish perioxidase) and its use in identification of spirochetes. J Histochem Cytochem 18:315–333

    Google Scholar 

  • Verley JM, Hollmann KH (1985) Thymoma. A comparative Study of clinical stages, histologic features and survival in 200 cases. Cancer 55:1074–1086

    Google Scholar 

  • Wekerle H, Müller-Hermelink HK (1985) The thymus in myasthenia gravis. In: Müller-Hermelink HK (ed) Histophysiology and pathology of the human thymus. Current Topics in Pathology, vol. 75, Springer Verlag, Berlin Heidelberg New York, in press

    Google Scholar 

  • Wijngaert FP van de, Kendall MD, Schuurman H-J, Rademakers LHPM, Kater L (1984) Heterogeneity of epithelial cells in the human thymus. An ultrastructural study. Cell Tissue Res 237:227–237

    Google Scholar 

  • Woda BA, Bain K, Salm T van der (1984) The phenotype of lymphocytes in a thymoma as studied with monoclonal antibodies. Clin Immunol Immunopathol 30:197–201

    Google Scholar 

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Authors and Affiliations

  1. Institute of Pathology, University of Würzburg, D-8700, Würzburg, Germany

    Hans Konrad Müller-Hermelink & Thomas Kirchner

  2. Department of Anatomy and Pathologic Histology, Ospedale S. Giacomo, I-00186, Rome, Italy

    Mirella Marino

  3. Institute of Pathology, University of Turin, I-10126, Turin, Italy

    Giorgio Palestro

  4. Anatomical Institute, University of Munich, D-8000, Munich, Germany

    Udo Schumacher

Authors
  1. Mirella Marino
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  2. Giorgio Palestro
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  3. Udo Schumacher
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  4. Thomas Kirchner
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This work has been supported by the Deutsche Forschungsgemeinschaft, SFB 111, project CN5

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Müller-Hermelink, H.K., Marino, M., Palestro, G. et al. Immunohistological evidences of cortical and medullary differentiation in thymoma. Vichows Archiv A Pathol Anat 408, 143–161 (1985). https://doi.org/10.1007/BF00707978

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