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Distribution of gelatinase B (MMP-9) and type IV collagen in colorectal carcinoma

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Abstract

Gelatinase B (92 kD matrix metalloproteinase-9, MMP-9), an enzyme capable of degrading several connective tissue components, was demonstrated by immunolocalization in all specimens of colorectal carcinoma (n=40), but its distribution between specimens was variable. MMP-9 expression was more frequently observed in advanced tumour stages and was especially prevalent at the side and deep margins of the tumours, and ulceration sites. MMP-9 staining was observed for transformed epithelial cells, macrophages and neutrophils, but seldom for vascular or stromal cells. By contrast, the enzyme was absent from epithelial cells of normal mucosal tissue. Immunostaining of type IV collagen, the major structural component of basement membranes, revealed a general depletion or loss of these structures both within the tumours and at the tumour margins. Dual immunolocalization of MMP-9 and type IV collagen demonstrated that MMP-9 expression at specific sites in the tumour was often inversely related to the distribution of type IV collagen MMP-9 expression was most pronounced at the invasive tumour margins and in microfoci where tumour cells were in close proximity to inflammatory cells. Such observations support the concept that localized proteolytic and collagenolytic activities contribute to the invasive properties of colorectal tumours.

Résumé

La gélatinase B (92 kD, matrice métalloprotéinase-9, MMP-9), une enzyme capable de dégrader de nombreux composants du tissu conjonctif a été mise en évidence par immuno-localisation dans des specimens de cancers colo-rectaux (n=40) mais sa distribution entre les divers échantillons était inégale. La mise en évidence de MMP-9 était plus fréquemment observée dans des tumeurs d'un stade avancé et particulièrement sur les berges et en profondeur de ces tumeurs et dans les zones ulcérées. La coloration du MMP-9 a été observée dans des cellules épithéliales transformées, des macrophages et des neutrophiles mais rarement dans des cellules vasculaires ou des cellules du stroma. Au contraire l'enzyme était absente des cellules épithéliales d'une muquese normale. L'immuno-coloration du collagène type IV, le composant structurel majeur des membranes basales, montrent une déplétion générale ou une perte de ces structures à la fois dans les tumeurs et dans les marges de celles-ci. La localisation par immuno-histochimie à la fois de MMP-9 et du type IV du collagène démontre que l'expression du MMP-9 dans certains sites tumoraux est souvent en relation inverse à la distribution du collagène de type IV. L'expression de MMP-9 était plus prononcée sur les berges d'une tumeur invasive et dans des microfoyers tumouraux lorsque les cellules tumorales étaient à proximité immédiate de cellules inflammatoires. Ces observations soutiennent le concept que des activités protéolytiques et collagénolytiques localisées contribuent à l'extansion des tumeurs colo-rectales.

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References

  1. Dukes CE, Bussey HJR (1958) The spread of the rectal cancer and its effect on prognosis. Br J Surg 12:309–320

    Google Scholar 

  2. Jass JR, Atkin WS, Cuzick J, Bussey HJR, Morson BC, Northover JMA, Todd IP (1986) The grading of rectal cancer: historical perspectives and multivariate analysis of 447 cases. Histopathology 10:437–459

    Google Scholar 

  3. Jass JR, Love SB, Northover JMA (1987) A new prognostic classification of rectal cancer. Lancet i:1303–1306

    Google Scholar 

  4. Hase K, Shatney C, Johnson D, Trollope M, Vierra M (1993) Prognostic value of tumour “budding” in patients with colorectal cancer. Dis Colon Rectum 36:627–635

    Google Scholar 

  5. Vracko R (1974) Basal lamina scaffold — anatomy and significance for maintenance of orderly tissue structure. Am J Pathol 77:314–346

    Google Scholar 

  6. Abrahamson DR (1986) Recent studies on the structure and pathology of basement membranes. J Pathol 149:257–278

    Google Scholar 

  7. Albrechtsen R, Wewer UM, Liotta LA (1986) Basement membranes in human cancer. Pathol Annu 21:251–276

    Google Scholar 

  8. Forster SJ, Talbot IC, Clayton DG, Critchley DR (1986) Tumour basement membrane laminin in adenocarcinoma of rectum: An immunohistochemical study of biological and clinical significance. Int J Cancer 37:813–817

    Google Scholar 

  9. Grigioni WF, Biagni G, Errico AD, Milani M, VillanacciV, Garbisa S, Mattioli S, Gozzetti C, Mancini AM (1986) Behaviour of basement membrane antigens in gastric and colorectal cancer. Acta Pathol Jpn 36:173–184

    Google Scholar 

  10. Hewitt RE, Powe DG, Griffin NR, Turner DR (1991) Relationship between epithelial basement membrane staining patterns in primary colorectal carcinomas and the extent of tumour spread. Int J Cancer 48:855–860

    Google Scholar 

  11. Birkedal-Hansen H, Moore WG, Bodden MK, Windsor LJ, Birkedal-Hansen B, DeCarlo A, Engler JA (1993) Matrix metalloproteinases: A review Crit Rev Oral Biol Med 4:197–250

    Google Scholar 

  12. Murphy G, Reynolds JJ (1993) Extracellular matrix degradation. In: Royce PM, Steinman B (eds) Connective tissue and its heritable disorders: Molecular and genetic aspects, Wiley-Liss Inc, New York, pp 287–316

    Google Scholar 

  13. Nagase H, Barrett AJ, Woessnar JF, Jr (1992) Nomenclature and glossary of the matrix metalloproteinases. In: Birkedal-Hansen H, Werb Z, Welgus HG, Van Wart HE (eds) Matrix Metalloproteinases and Inhibitors, Gustav Fischer, Stuttgart, pp 421–424

    Google Scholar 

  14. Morodomi T, Ogata Y, Sasaguri Y, Morimatsu M, Nagase H (1992) Purification and characterisation of matrix metalloproteinase-9 from U937 monocytic leukaemia and HT 1080 fibrosarcoma cells. Biochem J 285:603–611

    Google Scholar 

  15. Murphy G, Docherty AJP, Hembry RM, Reynolds JJ (1991) Metalloproteinases and tissue damage. Br J Rheumatol 30 (Suppl 1):25–31

    Google Scholar 

  16. Liotta LA, Rao CN, Barsky SH (1983) Tumour invasion and the extracellular matrix. Lab Invest 49:636–649

    Google Scholar 

  17. Van der Stappen JWJ, Hendriks T, Wobbes T (1990) Correlation between collagenolytic activity and grade of histological differentiation in colorectal tumours. Int J Cancer 45:1071–1078

    Google Scholar 

  18. Hewitt RE, Leach IH, Powe DG, Clark IM, Cawston TE, Turner DR (1991) Distribution of collagenase and tissue inhibitor of metalloproteinases (TIMP) in colorectal tumours. Int J Cancer 49:666–672

    Google Scholar 

  19. Poulsom R, Pignatelli M, Stetler-Stevenson WG, Liotta LA, Wright PA, Jeffrey RE, Longcroft JM, Rogers L, Stamp GWH (1992) Stromal expression of 72 kda type IV collagenase (MMP-2) and TIMP-2 mRNAs in colorectal neoplasia. Am J Pathol 141:389–396

    Google Scholar 

  20. D'Errico A, Garbisa S, LiottaLA, Castronova V, Stetler-Stevenson WG, Grigioni WF (1991) Augmentation of type IV collagenase, laminin receptor, and Ki67 proliferation antigen associated with human colon, gastric, and breast carcinoma progression. Mod Pathol 4:239–246

    Google Scholar 

  21. Ogata Y, Pratta MA, Nagase H, Arner EC (1992) Matrix metalloproteinase-9 (92-kDa gelatinase/type-IV collagenase) is induced in rabbit articular chondrocytes by cotreatment with interleukin 1-beta and a protein kinase C activator. Exp Cell Res 201:245–249

    Google Scholar 

  22. Morson BC, DawsonIMP, Day DW, Jass JR, Price AB, Williams GT (1991) Morson & Dawson's Gastrointestinal Pathology, 3rd edn. Blackwell, Oxford, pp 597–629

    Google Scholar 

  23. Jass JR (1992) Epithelial and mesenchymal tumours of the small and large bowel. In: McGee JO'D, Isaacson PG, Wright NA (eds) Oxford Textbook of Pathology, v 2 a, pp 1258–1281

  24. Burtin P, Chavanel G (1983) Immunofluorescence study of the antigens of the basement membrane and the peritumoral stoma in human colonic adenocarcinomas. Ann NY Acad Sci 420:229–236

    Google Scholar 

  25. Havenith MG, Arends JW, Simon R, Volovics A, Wiggers T, Bosman FT (1988) Type IV collagen immunoreactivity in colorectal cancer. Cancer 62:2207–2211

    Google Scholar 

  26. Barsky SH, Siegal GP, Jannotta F, Liotta LA (1983) Loss of basement membrane components by invasive tumours but not by their benign counterparts. Lab Invest 49:140–147

    Google Scholar 

  27. Nakamura K, Mori M, Enjoi M (1987) Distribution of basement membrane antigens in clinical gastric adenocarcinomas: an immunohistochemical study. J Clin Pathol 40:1418–1423

    Google Scholar 

  28. Nakajima M, Chop AM (1991) Tumour invasion and extracellular matrix degradative enzymes: regulation of activity by organ factors. Semin Cancer Biol 2:115–127

    Google Scholar 

  29. Nagase H, Ogata Y, Suzuki K, Enghild JJ, Salvesen G (1991) Substrate specificities and activation mechanisms of matrix metalloproteinases. Biochem Soc Trans 19:715–718

    Google Scholar 

  30. Senior RM, Griffin GL, Fliszar CJ, Shapiro SD, Goldberg GI, Welgus HG (1991) Human 92-kilodalton and 72-kilodalton type IV collagenases are elasteses. J Biol Chem 266:7870–7875

    Google Scholar 

  31. Woolley DE (1984) Collagenolytic mechanisms in tumour cell invasion. Cancer Metastasis Rev 3:361–372

    Google Scholar 

  32. Dabbous MK, Woolley DE, Hanley L, Carter LM, Nicolson GL (1986) Host-mediated effectors of tumour invasion: role of mast cells in matrix degradation. Clin Exp Metastasis 4:141–152

    Google Scholar 

  33. Garbisa S, D'Errico A, Grigioni WF, Biagni G, Caenazzo C, Fastelli G, Stetler-Stevenson W, Liotta LA (1990) Type IV collagenase augmentation associated with colorectal and gastric cancer progression. In: Harris CC (ed) Genetical Mechanisms in Carcinogenesis and Tumour Progression, Wiley-Liss, Inc., pp 203–212

  34. Pyke C, Ralfkiær E, Tryggvason K, DanøK (1993) Messenger RNA for two type IV collagenases is located in stromal cells in human colon cancer. Am J Pathol 142:359–365

    Google Scholar 

  35. Woessner JF Jr (1991) Matrix metalloproteinases and their inhibitors in connective tissue remodelling. FASEB J 5:2145–2154

    Google Scholar 

  36. Watanabe H, NakanishiI, Yamashita K, Hayakawa T, Okada Y (1993) Matrix metalloproteinase-9 (92 kDa gelatinase/type IV collagenase) from U937 monoblastoid cells: correlation with cellular invasion. J Cell Sci 104:991–999

    Google Scholar 

  37. Juarez J, Clayman G, Nakajima, M, Tanabe KK, Saya H, Nicolson GL (1993) Role and regulation of expression of 92-kDa type-IV collagenase (MMP-9) in 2 invasive squamous-cell-carcinoma cell lines of th eoral cavity. Int J Cancer 55:10–18

    Google Scholar 

  38. BernhardEJ, Muschel RJ, Hughes EN (1990) Mr 92,000 gelatinase release correlates with the metastatic phenotype in transformed rat embryo cells. Cancer Res 50:3872–3877

    Google Scholar 

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Authors and Affiliations

  1. Department of Medicine, University Hospital of South Manchester, M20 8LR, Manchester, UK

    M. Jeziorska & D. E. Woolley

  2. Department of Histopathology, University Hospital of South Manchester, M20 8LR, Manchester, UK

    N. Y. Haboubi

  3. Department of Surgery, University Hospital of South Manchester, M20 8LR, Manchester, UK

    P. F. Schofield

  4. Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, 66160-7421, Kansas City, KS, USA

    Y. Ogata & H. Nagase

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  1. M. Jeziorska
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  3. P. F. Schofield
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  4. Y. Ogata
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  5. H. Nagase
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  6. D. E. Woolley
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Jeziorska, M., Haboubi, N.Y., Schofield, P.F. et al. Distribution of gelatinase B (MMP-9) and type IV collagen in colorectal carcinoma. Int J Colorect Dis 9, 141–148 (1994). https://doi.org/10.1007/BF00290191

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