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Assignment of human transcobalamin II (TC2) to chromosome 22 using somatic cell hybrids and monosomic meningioma cells

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Summary

Human transcobalamin II (TC2), a vitamin B12 binding serum protein, is synthesized and secreted into the medium by cells growing in vitro. Mouse-man somatic cell hybrids were analyzed in order to map the locus of TC2. The presence of human TC2 in the culture media was correlated with the results of genetic marker and chromosome analysis of the hybrid cells. Chromosome 22 showed 100% concordancy. However, chromosome 6 (90% concordancy) and chromosome 7 (96% concordancy) were not completely excluded. Meningioma cells obtained from patients heterozygous for TC2 showed a concomitant loss of one chromosome 22 and one of the TC2 alleles, strongly supporting the assignment to chromosome 22.

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Authors and Affiliations

  1. Institute of Human Genetics, Free University, P.O. Box 7161, NL-1007 MC, Amsterdam, The Netherlands

    F. Arwert, H. J. Porck, R. R. Frants, H. T. Kortbeek & A. W. Eriksson

  2. Institut für Biochemie der Universität, Winterthurer Strasse 190, CH-8057, Zürich, Switzerland

    M. Fràter-Schröder

  3. Institute of Human Genetics, UCSC, Rome, Italy

    C. Brahe

  4. Department of Cell Biology and Genetics, Erasmus University, P.O. Box 1738, NL-3000 DR, Rotterdam, The Netherlands

    A. Geurts van Kessel & A. Westerveld

  5. Department of Human Genetics, University of Leiden, Sylvius Laboratories, NL-2333 AL, Leiden, The Netherlands

    P. Meera Khan

  6. Institut für Humangenetik der Universität des Saarlandes, Universität Bau 68, D-6650, Homburg/Saar, Germany

    K. Zang

Authors
  1. F. Arwert
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  2. H. J. Porck
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  3. M. Fràter-Schröder
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  4. C. Brahe
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  5. A. Geurts van Kessel
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  6. A. Westerveld
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  7. P. Meera Khan
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  8. K. Zang
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  9. R. R. Frants
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  10. H. T. Kortbeek
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  11. A. W. Eriksson
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Arwert, F., Porck, H.J., Fràter-Schröder, M. et al. Assignment of human transcobalamin II (TC2) to chromosome 22 using somatic cell hybrids and monosomic meningioma cells. Hum Genet 74, 378–381 (1986). https://doi.org/10.1007/BF00280489

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  • DOI: https://doi.org/10.1007/BF00280489

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