Abstract
Cytokine measurement is a prerequisite to understand the inflammatory state of the body. Quantitative analysis of cytokines by Western blotting and ELISA is a daunting task as these are time-consuming and error-prone protocols. With the advent of flow cytometry, the estimation of cytokines using the classical antigen–antibody reaction has become a popular choice with researchers/clinicians. Here, we describe a protocol for multiple cytokine analysis using flow cytometry.
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Kaushik, D.K., Basu, A. (2013). Microglial Activation: Measurement of Cytokines by Flow Cytometry. In: Joseph, B., Venero, J. (eds) Microglia. Methods in Molecular Biology, vol 1041. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-520-0_9
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DOI: https://doi.org/10.1007/978-1-62703-520-0_9
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-519-4
Online ISBN: 978-1-62703-520-0
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