Abstract
Primary cultures are an important in vitro tool to study cellular processes and interactions. These cultures are complex systems, composed of many cell types, including neurons, astrocytes, oligodendrocytes, microglia, NG2 cells, and endothelial cells. For some studies it is necessary to be able to study a pure culture of one cell type, or eliminate a particular cell type, to better understand its function. There exist cell culture protocols for making pure astrocyte or microglia cultures. Here we present two protocols to produce cultures depleted for microglia: in the first case, from a mixed astrocyte–microglia culture and, in the second, for eliminating microglia from neuronal cultures.
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Acknowledgments
We thank Sarrah Ben Achour for preliminary experiments and thank Yasmine Belarif-Cantaut for the helpful discussion with the Saporin protocol. The work was supported by a grant from the seventh Framework Program Moodinflame (222963).
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Pont-Lezica, L., Colasse, S., Bessis, A. (2013). Depletion of Microglia from Primary Cellular Cultures. In: Joseph, B., Venero, J. (eds) Microglia. Methods in Molecular Biology, vol 1041. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-520-0_7
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DOI: https://doi.org/10.1007/978-1-62703-520-0_7
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