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Assessing Urinary Tract Defects in Mice: Methods to Detect the Presence of Vesicoureteric Reflux and Urinary Tract Obstruction

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Kidney Development

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 886))

Abstract

Congenital Anomalies of the Kidney and Urinary Tract (CAKUT) encompass a spectrum of kidney and urinary tract disorders. Here, we describe two assays that can be used to determine if a mouse has vesicoureteric reflux (VUR) or urinary tract obstruction, two urinary tract defects observed in CAKUT. To test for VUR, dye is injected into the mouse bladder and then monitored to determine if it passes retrogradely from the bladder towards the kidneys, indicating the presence of VUR. To test for urinary tract obstruction, the renal pelvis is microinjected with dye and its passage along the urinary tract is monitored to determine if there is evidence of impaired flow along the tract. These methods will facilitate the analysis of CAKUT phenotypes in the mouse.

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Authors and Affiliations

  1. Department of Human Genetics, Montreal Children’s Hospital, McGill University, Montreal, QC, Canada

    Inga J. Murawski & Christine L. Watt

  2. Department of Human Genetics and Pediatrics, Montreal Children’s Hospital, McGill University, Montreal, QC, Canada

    Indra R. Gupta

Authors
  1. Inga J. Murawski
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  2. Christine L. Watt
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  3. Indra R. Gupta
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Corresponding author

Correspondence to Indra R. Gupta .

Editor information

Editors and Affiliations

  1. Hammer Health Sciences Center Department of Genetics and Development, Columbia University Medical Center, New York, NY, USA

    Odyssé Michos

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Murawski, I.J., Watt, C.L., Gupta, I.R. (2012). Assessing Urinary Tract Defects in Mice: Methods to Detect the Presence of Vesicoureteric Reflux and Urinary Tract Obstruction. In: Michos, O. (eds) Kidney Development. Methods in Molecular Biology™, vol 886. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-851-1_31

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  • DOI: https://doi.org/10.1007/978-1-61779-851-1_31

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-850-4

  • Online ISBN: 978-1-61779-851-1

  • eBook Packages: Springer Protocols

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