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NMR and MS Methods for Metabonomics

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Drug Safety Evaluation

Part of the book series: Methods in Molecular Biology ((MIMB,volume 691))

Abstract

Metabonomics, also often referred to as “metabolomics” or “metabolic profiling,” is the systematic profiling of metabolites in bio-fluids or tissues of organisms and their temporal changes. In the last decade, metabonomics has become increasingly popular in drug development, molecular medicine, and other biotechnology fields, since it profiles directly the phenotype and changes thereof in contrast to other “-omics” technologies. The increasing popularity of metabonomics has been possible only due to the enormous development in the technology and bioinformatics fields. In particular, the analytical technologies supporting metabonomics, i.e., NMR, LC-MS, UPLC-MS, and GC-MS have evolved into sensitive and highly reproducible platforms allowing the determination of hundreds of metabolites in parallel. This chapter describes the best practices of metabonomics as seen today. All important steps of metabolic profiling in drug development and molecular medicine are described in great detail, starting from sample preparation, to determining the measurement details of all analytical platforms, and finally, to discussing the corresponding specific steps of data analysis.

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Authors and Affiliations

  1. Molecular Diagnostics, Novartis Pharma AG, Basel, Switzerland

    Frank Dieterle

  2. Laboratory Diagnostics, Non-Clinical Drug Safety Evaluation, Bayer Schering Pharma AG, Berlin, Germany

    Björn Riefke

  3. Hoffmann LaRoche, Basel, Switzerland

    Götz Schlotterbeck, Alfred Ross & Hans Senn

  4. Disposition, Safety and Animal Research, sanofi-aventis R&D, Hattersheim, Germany

    Alexander Amberg

Authors
  1. Frank Dieterle
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  2. Björn Riefke
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  3. Götz Schlotterbeck
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  4. Alfred Ross
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  5. Hans Senn
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  6. Alexander Amberg
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Editor information

Editors and Affiliations

  1. , Toxicologie Cellulaire et Moléculaire, Sanofi-aventis, Quai Jules Guesde 13, Vitry-sur-Seine, 94400, France

    Jean-Charles Gautier

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Dieterle, F., Riefke, B., Schlotterbeck, G., Ross, A., Senn, H., Amberg, A. (2011). NMR and MS Methods for Metabonomics. In: Gautier, JC. (eds) Drug Safety Evaluation. Methods in Molecular Biology, vol 691. Humana Press. https://doi.org/10.1007/978-1-60761-849-2_24

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  • DOI: https://doi.org/10.1007/978-1-60761-849-2_24

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60327-186-8

  • Online ISBN: 978-1-60761-849-2

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