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Antibody Epitope Mapping Using De Novo Generated Synthetic Peptide Libraries

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Epitope Mapping Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 524))

Summary

Identification of antibody binding peptides may be based on the primary structure of the protein antigens used to raise the antibodies (knowledge- or sequence-based approach). This involves scanning the entire sequence of the antigen with overlapping peptides (peptide scan), which are then probed for binding to the respective antibody. If a natural protein binding partner is not known, one has to use combinatorial synthetic libraries with peptide mixtures, randomly generated chemically synthesized libraries of single individual sequences, or biologically produced libraries (e.g., phage display libraries, see Chapter “Epitope Mapping Using Phage Display Peptide Libraries”). This chapter describes chemically synthesized combinatorial, as well as randomly generated peptide libraries, collectively called de novo approaches, and their application for antibody epitope mapping.

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  1. Lead Discovery Biology Department, Jerini AG, Invalidenstr. 130, D-10115, Berlin, Germany

    Ulrich Reineke

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  1. Ulrich Reineke
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Correspondence to Ulrich Reineke .

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    © 2009 Humana Press, a part of Springer Science+Business Media, LLC

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    Reineke, U. (2009). Antibody Epitope Mapping Using De Novo Generated Synthetic Peptide Libraries. In: Schutkowski, M., Reineke, U. (eds) Epitope Mapping Protocols. Methods in Molecular Biology™, vol 524. Humana Press. https://doi.org/10.1007/978-1-59745-450-6_14

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    • DOI: https://doi.org/10.1007/978-1-59745-450-6_14

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    • Publisher Name: Humana Press

    • Print ISBN: 978-1-934115-17-6

    • Online ISBN: 978-1-59745-450-6

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