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Monocyte-Derived Dendritic Cells as Antigen-Presenting Cells in T-Cell Proliferation and Cytokine Production

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Allergy

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2020))

Abstract

Dendritic cells (DC) are the most potent antigen-presenting cells that link innate with adaptive immunity. They circulate the body and sample the microenvironments for maintaining homeostasis and for mounting T-cell responses against invading pathogens, foreign antigens, and aberrant self-proteins. In humans, DC derived from blood monocytes (MDC) by cytokine treatment provide the most abundant and versatile source for studying DC and T-cell biology, and for use as adjuvants in cancer therapy. In asthma patients, T-cell functions are studied by using autologous MDC as accessory cells for allergen presentation. The method for isolating T cells and monocytes from peripheral blood mononuclear cells (PBMC) and the stimulation of T cells to proliferate and produce cytokines by MDC are outlined in this chapter. The method can be applied to the functional studies of T cells and DC in other diseases.

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Authors and Affiliations

  1. Department of Medicine, Center for Immunity, Inflammation, and Regenerative Medicine, University of Virginia Health Sciences Center, Charlottesville, VA, USA

    Sun-Sang J. Sung

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  1. Sun-Sang J. Sung
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Correspondence to Sun-Sang J. Sung .

Editor information

Editors and Affiliations

  1. Core Facilities, St. George’s University of London, London, UK

    Penny Lympany

  2. Department of Occupational and Environmental Medicine, Imperial College London, London, UK

    Meinir G. Jones

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Sung, SS.J. (2019). Monocyte-Derived Dendritic Cells as Antigen-Presenting Cells in T-Cell Proliferation and Cytokine Production. In: Lympany, P., Jones, M. (eds) Allergy. Methods in Molecular Biology, vol 2020. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9591-2_9

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  • DOI: https://doi.org/10.1007/978-1-4939-9591-2_9

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9589-9

  • Online ISBN: 978-1-4939-9591-2

  • eBook Packages: Springer Protocols

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