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Insights into the NOX NADPH Oxidases Using Heterologous Whole Cell Assays

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NADPH Oxidases

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1982))

Abstract

Assays based on ectopic expression of NOX NADPH oxidase subunits in heterologous mammalian cells are an important approach for investigating features of this family of enzymes. These model systems have been used to analyze the biosynthesis and functional domains of NOX enzyme components as well as their regulation and cellular activities. This chapter provides an overview of the basic principles and applications of heterologous whole cell assays in studying NOX NADPH oxidases.

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Acknowledgments

MCD is funded by the Children’s Discovery Institute at Washington University and St. Louis Children’s Hospital and NIH grants R01HL045635 and R01AR072212. I thank Tina McGrath and Diane Jensen for assistance with preparation of the manuscript. I apologize to colleagues whose work could not be cited due to space limitations.

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Authors and Affiliations

  1. Department of Pediatrics, Washington University in St. Louis School of Medicine , St. Louis, MO, USA

    Mary C. Dinauer

  2. Department of Pathology and Immunology, Washington University in St. Louis School of Medicine, St. Louis, MO, USA

    Mary C. Dinauer

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  1. Mary C. Dinauer
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Correspondence to Mary C. Dinauer .

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Editors and Affiliations

  1. Conway Institute, School of Medicine, University College Dublin, Dublin, Ireland

    Ulla G. Knaus

  2. Laboratory of Clinical Immunology and Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA

    Thomas L. Leto

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Dinauer, M.C. (2019). Insights into the NOX NADPH Oxidases Using Heterologous Whole Cell Assays. In: Knaus, U., Leto, T. (eds) NADPH Oxidases. Methods in Molecular Biology, vol 1982. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9424-3_9

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  • DOI: https://doi.org/10.1007/978-1-4939-9424-3_9

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