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Ice Crystals in Tissues and Organs

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The Biophysics of Organ Cryopreservation

Part of the book series: NATO ASI Series ((NSSA,volume 147))

Abstract

It is generally supposed that extracellular ice is innocuous to slowly-frozen cells — that freezing damage is a consequence either of reduction in temperature per se, or of changes in solution composition occasioned by freezing, or both. There are many papers supporting this view, but those of Lovelock (1,2), Meryman (3), Farrant and Morris (4) and Mazur (5) will suffice. However, this comfortable consensus has recently been disturbed by Mazur and his colleagues (6,7,8,9) who now advocate a direct, presumbly mechanical, action by extracellular ice. These workers have provided extensive experimental evidence which they believe indicates that reduction in the fraction of water that remains unfrozen is more damaging than the increase in solute concentration that accompanies freezing: they discuss mechanisms such as crushing of cells within the narrow liquid channels between the ice masses, and forced cell-to-cell contacts.

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Author information

Authors and Affiliations

  1. MRC Medical Cryobiology Group, University Department of Surgery, Douglas House, Trumpington Road, Cambridge, CB2 2AH, UK

    David E. Pegg

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  1. David E. Pegg
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Editor information

Editors and Affiliations

  1. MRC Medical Cryobiology Group, Cambridge University, Cambridge, UK

    David E. Pegg

  2. Medical College of Georgia, Augusta, Georgia, USA

    Armand M. Karow Jr.

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© 1987 Plenum Press, New York

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Pegg, D.E. (1987). Ice Crystals in Tissues and Organs. In: Pegg, D.E., Karow, A.M. (eds) The Biophysics of Organ Cryopreservation. NATO ASI Series, vol 147. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5469-7_7

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  • DOI: https://doi.org/10.1007/978-1-4684-5469-7_7

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-5471-0

  • Online ISBN: 978-1-4684-5469-7

  • eBook Packages: Springer Book Archive

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