Abstract
Transmissible gastroenteritis coronavirus (TGEV) infects both, the enteric and the respiratory tract of swine. S protein, that is recognized by the cellular receptor, has been proposed that plays an essential role in controlling the dominant tropism.The genetic relationship of S gene from different enteric strains and non-enteropathogenic porcine respiratory coronaviruses (PRCVs) was determined. A correlation between tropism and the genetic structure of the S gene was established. PRCVs, derived from enteric isolates have a large deletion at the N-terminus of the S protein. Interestingly, two respiratory isolates, attenuated Purdue type virus (PTV-ATT) and Toyama (TOY56) have a full-length S gene. PTV-ATT has two specific amino acid differences with the S protein of the enteric viruses. One is located around position 219, within the deleted area, suggesting that alterations around this amino acid may result in the loss of enteric tropism.
To study the role of different genes in tropism, a cluster of viruses closely related to PUR46 strain was analyzed. All of them have been originated by accumulating point mutations from a common, virulent isolate which infected the enteric tract. During their evolution these viruses have lost, virulence first, and then, enteric tropism. Sequencing analysis proved that enteric tropism could be lost without changes in ORFs 3a, 3b, 4, 6, and 7, and in 3′-end untranslated regions (3’-UTR). To study the role of the S protein in tropism recombinants were obtained between an enteric and a respiratory virus of this cluster. Analysis of the recombinants supported the hypothesis on the role in tropism of S protein domain around position 219.
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References
Bohl, E. H. Antibody in serum, colostrum, and milk of swine after infection or vaccination with transmissible gastroenteritis virus. Infect. Immun. 1972; 6: 289–301.
Britton, P., Page, K. W., Mawditt, K., Pocock, D. H. Sequence comparison of porcine transmissible gastroenteritis virus (TGEV) with porcine respiratory coronavirus. VHIth International Congress of Virology. 1990. IUMS, Berlin, pp. P6–018.
Callebaut, P., Correa, I., Pensaert, M., Jiménez, G., Enjuanes, L. Antigenic differentiation between transmissible gastroenteritis virus of swine and a related porcine respiratory coronavirus. J. Gen. Virol. 1988; 69: 1725–1730.
Delmas, B., Gelfi, J., L’Haridon, R., Vogel, L. K., Norén, O., Laude, H. Aminopeptidase N is a major receptor for the enteropathogenic coronavirus TGEV. Nature 1992; 357: 417–420.
Fichot, O., Girard, M. An improved method for sequencing of RNA templates. Nucleic Acids Res. 1990;18:6162.
Haelterman, E. O., Pensaert, M. B. Pathogenesis of transmissible gastroenteritis of swine. Proc. 18th World Vet. Congress. 1967. Paris. Vol. 2, pp. 569–572.
Jiménez, G., Correa, I., Melgosa, M. P., Bullido, M. J., Enjuanes, L. Critical epitopes in transmissible gastroenteritis virus neutralization. J. Virol. 1986; 60: 131–139.
Laude, H., Godet, M., Bernard, S., Gelfi, J., Duarte, M., Delmas, B. Functional domains in the spike protein of transmissible gastroenteritis virus. VI International symposium on corona-and related viruses.1994. Québec, Canada.
Laude, H., Vanreeth, K., Pensaert, M. Porcine respiratory coronavirus -molecular features and virus host interactions. Vet. Res. 1993; 24: 125–150.
Makino, S., Keck, J. G., Stohlman, S. A., Lai, M. M. C. High-frequency RNA recombination of murine coronaviruses. J. Virol. 1986; 57: 729–737.
McClurkin, A. W., Norman, J. O. Studies on transmissible gastroenteritis of swine II. Selected charac-teristics of a cytopathogenic virus common to five isolates from transmissible gastroenteritis. Can. J.Comp. Vet. Sci. 1966; 30: 190–198.
Pensaert, M., Callebaut, P., Vergote, J. Isolation of a porcine respiratory, non-enteric coronavirus related to transmissible gastroenteritis. Vet. Q. 1986; 8: 257–260.
Rasschaert, D., Duarte, M., Laude, H. Porcine respiratory coronavirus differs from transmissible gastroenteritis virus by a few genomic deletions. J. Gen. Virol. 1990; 71: 2599–2607.
Sánchez, C. M., Gebauer, F., Suñe, C., Méndez, A., Dopazo, J., Enjuanes, L. Genetic evolution and tropism of transmissible gastroenteritis coronaviruses. Virology 1992; 190: 92–105.
Sánchez, C. M., Jiménez, G., Laviada, M. D., Correa, I., Suñé, C., Bullido, M. J., Gebauer, F., Smerdou, C, Callebaut, P., Escribano, J. M., Enjuanes, L. Antigenic homology among coronaviruses related to transmissible gastroenteritis virus. Virology 1990; 174: 410–417.
Suñé, C., Jiménez, G., Correa, L, Bullido, M. J., Gebauer, F., Smerdou, C., Enjuanes, L. Mechanisms of transmissible gastroenteritis coronavirus neutralization. Virology 1990; 177: 559–569.
Wesley, R. D., Woods, R. D., Cheung, A. K. Genetic analysis of porcine respiratory coronavirus, an attenuated variant of transmissible gastroenteritis virus. J. Virol. 1991; 65: 3369–3373.
Wesley, R. D., Woods, R. D., Hill, H. T., Biwer, J. D. Evidence for a porcine respiratory coronavirus, antigenically similar to transmissible gastroenteritis virus, in the United States. J. Vet. Diagn. Invest. 1990; 2:312–317.
Yokomori, K., Asanaka, M., Stohlman, S. A., Lai, M. M. C. A spike protein-dependent cellular factor other than the viral receptor is required for mouse hepatitis virus entry. Virology 1993; 196: 45–56.
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Ballesteros, M.L., Sánchez, C.M., Martín-Caballero, J., Enjuanes, L. (1995). Molecular Bases of Tropism in the PUR46 Cluster of Transmissible Gastroenteritis Coronaviruses. In: Talbot, P.J., Levy, G.A. (eds) Corona- and Related Viruses. Advances in Experimental Medicine and Biology, vol 380. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1899-0_89
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