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Methods for the Determination of Antioxidant Capacity in Food and Raw Materials

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Bio-Farms for Nutraceuticals

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 698))

Abstract

A comprehensive description of the most frequently used methods to determine the antioxidant activity in food and raw materials is given. The methods are classified into two categories, depending on the type of the assessment carried out. Several methods for the assessment of antioxidant efficacy using free radical scavenging such as Oxygen Radical Absorbance Capacity Assay (ORAC), Total Radical Trapping Antioxidant Parameter assay (TEAC), Ferric reducing antioxidant power assay (FRAP) and 2,2′-diphenyl-1-picrylhydrazyl (DPPH) assay are described. An example of methods based on the assessment of antioxidant efficacy using significant biological substrates is also presented. Critical opinions concerning the proposed methods are presented.

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Author information

Authors and Affiliations

  1. Centre of Bioanalysis, National Institute for Biological Sciences Bucharest, 296 Splaiul Independentei, Bucharest 6, 060031, Romania

    Simona Carmen Litescu

Authors
  1. Simona Carmen Litescu
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  2. Sandra Eremia
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  3. Gabriel Lucian Radu
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Editor information

Editors and Affiliations

  1. Institute of Crystallography, Department of Agrofood and Department of Molecular Design, National Council of Research, Area of Research of Rome, Monterotondo Scalo, Rome, Italy

    Maria Teresa Giardi PhD  & Giuseppina Rea PhD  & 

  2. Institute of General Physiology and Biochemistry, Department of Molecular Sciences Applied to Biosystems, DISMAB, University of Milan, Milan, Italy

    Bruno Berra PhD

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© 2010 Landes Bioscience and Springer Science+Business Media, LLC

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Litescu, S.C., Eremia, S., Radu, G.L. (2010). Methods for the Determination of Antioxidant Capacity in Food and Raw Materials. In: Giardi, M.T., Rea, G., Berra, B. (eds) Bio-Farms for Nutraceuticals. Advances in Experimental Medicine and Biology, vol 698. Springer, Boston, MA. https://doi.org/10.1007/978-1-4419-7347-4_18

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