Abstract
Chicken anemia virus (CAV) is an important pathogen associated with immunosuppression in chicken. In this study, out of samples collected from 115 commercial poultry farms, 12 samples were CAV positive by PCR. Partial sequence and phylogenetic analysis of VP1 gene revealed that the detected viruses were clustered to genotype I (n = 3) and genotype II (n = 9). Motifs of both low (E144) and high pathogenic strains (T89, I125, Q141) were found in the three viruses of genotype I. Whereas genotype II viruses demonstrated the characteristic motifs of highly pathogenic strains (I75, T89, I125, Q141, and Q144). Three isolates representative of both genotypes (CAV/CA1, CAV/GZ1 and CAV/SK4) were selected for full genome sequencing and results revealed that the VP2 gene had two substitutions at V153 and E 175, while VP3 gene had only one substitution at C118. To evaluate virus pathogenicity, two isolates from each genotype (CAV/SK4 of genotype I and CAV/CA1 of genotype II) were intramuscularly inoculated in two groups of one-day-old specific pathogen free chicks. Eighteen days post inoculation, PCR detected CAV in 75 and 90% of chicks in group I and II; respectively. Mortalities in inoculated chicks were 5 and 20% and packed cell volume values were 0.21 and 0.19; respectively. CAV/CA1 and CAV/SK4 isolates showed pathogenic evidences at the level of genetic (Q141 and 394Q) with variable degree of virulence. In conclusion, the study reports the circulation of at least two genotypes of CAV among chicken population with mutation associated with pathogenicity.
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AboElkhair M, Abd El-Razak AG, Metwally AY. Molecular characterization of chicken anemia virus circulating in chicken flocks in Egypt. Adv Virol. 2014;797151–6.
Adair BM. Immunopathogenesis of chicken anemia virus infection. Dev Comp Immunol. 2000;24:247–55.
Akiyama Y, Kato S. Two cell lines from lymphomas of Marek’s disease. Biken J. 1974;17:105–16.
Amin AA, Hassan MK, Mona M, Abdel-Zaher, A. A serological study of the prevalence of chicken anaemia in commercial flocks. In: Proceedings of the 5th science conference, Egypt veterinary poultry association; 1998. p. 69–75.
Dhama K, Kataria JM, Senthilkumar N, Dash BB, Tomar S. Standardization and application of polymerase chain reaction and indirect immunofluorescent technique for detection of chicken infectious anaemia virus. Indian J Comp Microbiol Immunol Infect Dis. 2002;23:11–22.
Dhama K, Mahendran M, Somvanshi R, Chawak MM. Chicken infectious anaemia virus: an immunosuppressive pathogen of poultry—a review. Indian J Vet Pathol. 2008;32:158–67.
Ducatez MF, Owoade AA, Abiola JO, Muller CP. Molecular epidemiology of chicken anemia virus in Nigeria. Arch Virol. 2005;151:97–111. https://doi.org/10.1007/s00705-005-0609-7.
El-Lethi AM. Viral contamination of dressed poultry. M.V.Sc. thesis, Cairo University, Egypt; 1990.
Ezzi A, Shoushtari A, Marjanmehr H, Toroghi R, Tavasoly A, Bahmani-nejad MA. Experimental studies of pathogenecity of chicken infectious anaemia virus (3 isolates) in Iran Archives of Razi Institute, Vol. 67, No. 1, June 13–19; 2012.
Hegazy AM, Abdallah FM, Abd-El Samie LK and Nazim AA. Incidence of Chicken Anemia Virus in Sharkia governorate chicken flocks. Assiut Vet Med J. 2014;752–61(142)
Hussein HA, Sabry MZ, El-Ebiary EA, El-Safty M, Abdel-Hady AL. Chicken infectious anemia virus in Egypt: molecular diagnosis by PCR and isolation of the virus from infected flocks. Ar J Biotechnol. 2002;5(2):263–74.
Islam MR, Johne R, Raue R, Todd D, Muller H. Sequence analysis of the full-length cloned DNA of a chicken anaemia virus (CAV) strain from Bangladesh: evidence for genetic grouping of CAV strains based on the deduced VP1 amino acid sequences. J Vet Med B Infect Dis Vet Public Health. 2002;49:332–7.
Jeurissen SHM, Wagenaar F, Pol JMA, van der Eb AJ, Noteborn MHM. Chicken anemia virus causes apoptosis of thymocytes after in vivo infection and of cell lines after in vitro infection. J Virol. 1992;66:7383–8.
Kim HR, Kwon YK, Bae YC, Oem JK, Lee OS. Molecular characterization of chicken infectious anemia viruses detected from breeder and broiler chickens in South Korea. Poult Sci. 2010;89:2426–31.
Koch G, van Roozelaar DJ, Verschueren CA, van der Eb AJ, Noteborn MH. Immunogenic and protective properties of chicken anaemia virus proteins ex pressed by baculovirus. Vaccine. 1995;8:763–70.
Kye S-J, Kim J-Y, Seul H-J, Kim S, Kim S-E, Lee H-S, Sorn S, Choi K-S. Phylogenetic analysis and genetic characterization of chicken anemia virus isolates from Cambodia. Poult Sci. 2013;92(10):2681–6.
Lai GH, Lin MK, Lien YY, Fu JH, Chen HJ, Huang CH, Tzen J, Lee MS. Expression and characterization of highly antigenic domains of chicken anemia virus viral VP2 and VP3 subunit proteins in a recombinant E. coli for sero-diagnostic applications. BMC Vet Res. 2013;9:161.
Liu ZG, Zheng SM, Xu YB, Zhou ZY, Wang XR, Yang LP. Immunopathological changes of chicken infectious anemia. Sci Agric Sin. 1997;30:74–82.
McNulty MS, Connor TJ, McNeilly F, McLoughlin MF, Kirkpatrick KS. Preliminary characterization of isolates of chicken anaemia agent from the United Kingdom. Avian Pathol. 1990;19:67–73.
Noteborn MHM, Koch G. Chicken anaemia virus infection: molecular basis of pathogenicity. Avian Pathol. 1995;24:11–31.
Pringle CR. Virus taxonomy at the XI international congress of virology, Sydney, Australia. Arch Virol. 1999;144:2065–70.
Reed LJ, Muench H. A simple method of estimating fifty percent endpoints. Am J Hyg. 1938;27:493–7.
Renshaw RW, Some C, Weinkle T, O’Connel PH, Ohashi K, Watson S, Lucio B, Harrington S, Schat KA. A hypervariable region in VP1 of chicken infectious anemia virus mediates rate of spread and cell tropism in tissue culture. J Virol. 1996;70:8872–8.
Schat KA. Chicken infectious anemia. In: Saif YM, editor. Diseases of poultry. Iowa: Iowa State Press; 2003. p. 182–202.
Schat KA, van Santen V. Chicken infectious anemia. In: Saif YM, Barns HJ, Gilssion JR, Fadly AM, McDougald LR, Swayne DE, editors. Disease of poultry. 11th ed. Ames: Iowa State University Press; 2009.
Sommer F, Cardona C. Chicken anemia virus in broilers: dynamics of the infection in two commerical broiler flocks. Avian Dis. 2003;47:1466–73.
Spackman E, Cloud SS, Rosenberger JK. Comparison of a putative second serotype of chicken infectious anemia virus with a prototypical isolate II. Antigenic and physicochemical characteristics. Avian Dis. 2002;46:956–63.
Todd D, Niagro FD, Ritchie BW, Curran W, Allan GM, Lukert PD, Latimer KS, Steffens WL III, McNulty MS. Comparison of three animal viruses with circular single-stranded DNA genomes. Arch Virol. 1991;117:129–35.
Todd D, Scott AN, Ball NW, Borghmans BJ, Adair BM. Molecular basis of the attenuation exhibited by molecularly cloned highly passaged chicken anemia virus isolates. J Virol. 2002;76:8472–4.
Toro H, Ramirez AM, Larenas J. Pathogenicity of chicken anemia virus (isolate 10343) for young and older chickens. Avian Pathol. 1997;26:485–99.
Von Bulow V. Infectious anemia. In: Calnek BW, editor. Diseases of poultry. 9th ed. Ames: Iowa State University Press; 1991. p. 690–9.
Yamaguchi S, Imada T, Kaji N, Mase M, Tsukamoto K, Tanimura N, Yuasa N. Identification of a genetic determinant of pathogenicity in chicken anaemia virus. J Gen Virol. 2001;82:1233–8.
Yuasa N. Propagation and infectivity titration of the Gifu-1 strain of chicken anemia agent in a cell line (MDCC-MSB1) derived from Marek’s disease lymphoma. Natl Inst Anim Health Q. 1983;23:13–20.
Yuasa N, Taniguchi T, Yoshida I. Isolation and some characteristics of an agent inducing anemia in chicks. Avian Dis. 1979;23:366–85.
Zhang X, Liu Y, Wu B, Sun B, Chen F, Ji J, Ma J, Xie Q. Phylogenetic and molecular characterization of chicken anemia virus in southern China from 2011 to 2012. Sci Rep. 2013;3:3519.
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The authors would like to acknowledge Naglaa Hagag, Marwa Ali, Ali Zanaty and the staff of gene analysis unit of reference laboratory of poultry production (RLQP) for the kind support.
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Abdel-Mawgod, S., Adel, A., Arafa, AS. et al. Full genome sequences of chicken anemia virus demonstrate mutations associated with pathogenicity in two different field isolates in Egypt. VirusDis. 29, 333–341 (2018). https://doi.org/10.1007/s13337-018-0467-z
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DOI: https://doi.org/10.1007/s13337-018-0467-z