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Transcriptomic analysis of the red and green light responses in Columba livia domestica

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Abstract

In this study, 108 paired White King pigeons, randomly divided into three compartments were exposed to green light, red light, and white light followed by 15 h of light exposure, for a 6-month period. Three female birds from each group were selected and ovarian stromal tissue was collected. Pigeon reproductive data were also recorded every day. We performed transcriptome assembly on several tissue samples using Illumina Hiseq 2000 and analyzed differentially expressed genes involving follicle development mechanisms. Reproductive data confirmed that exposure to red and green lights improved pigeon reproduction. In total, approximately 158,080 unigenes with an average length of 753 bp were obtained using the Trinity program. Gene ontology, clusters of orthologous groups, and the Kyoto encyclopedia of genes were used to annotate and classify these unigenes. Large numbers of differentially expressed genes were discovered through pairwise comparisons between groups treated with monochromatic light versus white light. Some of these genes are associated with steroid hormone biosynthesis, cell cycle and circadian rhythm. Furthermore, qRT-PCR was used to detect the relative expression levels of randomly selected genes. A total of 17,419 potential simple sequence repeats were also identified. Our study provides insights into potential molecular mechanisms and genes that regulate pigeon reproduction in response to monochromatic light exposure. Our results and data will facilitate a further investigation into the molecular mechanisms behind the effects of red and green lights on follicle development and reproduction in the pigeon.

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Data availability

Data are available at NCBI Transcriptome Shotgun Assembly Sequence Database (R1: SRR2148830, R2: SRR2148831, R3: SRR2148832; G1: SRR2148833, G2: SRR2148834, G3: SRR2148835; W1: SRR2094777, W2: SRR2094789, W3: SRR2094799).

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Acknowledgements

We would like to thank the National Science Foundation for Young Scientists of China (Grant No. 31702155) and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD; Jiangsu, China).

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Authors and Affiliations

  1. College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, Jiangsu Province, China

    Ying Wang, Haiming Yang & Zhiyue Wang

  2. Department of Pathology, Linyi People’s Hospital, Linyi, 276000, Shandong Province, China

    Chen Zi

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  1. Ying Wang
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  2. Haiming Yang
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  4. Zhiyue Wang
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Correspondence to Haiming Yang.

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Wang, Y., Yang, H., Zi, C. et al. Transcriptomic analysis of the red and green light responses in Columba livia domestica. 3 Biotech 9, 20 (2019). https://doi.org/10.1007/s13205-018-1551-1

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