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Plasminogen activator inhibitor type 1 expression induced by lipopolysaccharide of Porphyromonas gingivalis in human gingival fibroblast

  • Microbial Pathogenesis and Host-Microbe Interaction
  • Published:
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Abstract

In the gingival tissues of patients with periodontitis, inflammatory responses are mediated by a wide variety of genes. In our previous screening study, plasminogen activator inhibitor type 1 (PAI-1) mRNA binding protein expression was increased in gingiva from periodontitis patients. In this study, we further investigated the signaling pathway involved in PAI-1 expression induced by Porphyromonas gingivalis LPS (Pg LPS) in human gingival fibroblasts (HGF). When HGFs were treated with Pg LPS, both PAI-1 mRNA expression and PAI-1 protein were induced in a dose-dependent manner. Pg LPS induced NF-κB activation and the expressions of PAI-1 mRNA and protein were suppressed by pretreating with a NF-κB inhibitor. Pg LPS also induced ERK, p38, and JNK activation, and Pg LPS-induced PAI-1 expression was inhibited by ERK/p38/JNK inhibitor pretreatment. In conclusion, Pg LPS induced PAI-1 expression through NF-κB and MAP kinases activation in HGF.

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Authors and Affiliations

  1. Department of Oral Microbiology, Pusan National University, Yangsan, 626-810, Republic of Korea

    Hee Sam Na, Eun J. Lim, So Y. Jeong, Mi Hee Park & Jin Chung

  2. Department of Oral Pathology, School of Dentistry, Pusan National University, Yangsan, 626-810, Republic of Korea

    Mi H. Ryu

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  1. Hee Sam Na
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  2. Eun J. Lim
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  3. So Y. Jeong
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  4. Mi H. Ryu
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  5. Mi Hee Park
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  6. Jin Chung
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Correspondence to Jin Chung.

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Na, H.S., Lim, E.J., Jeong, S.Y. et al. Plasminogen activator inhibitor type 1 expression induced by lipopolysaccharide of Porphyromonas gingivalis in human gingival fibroblast. J Microbiol. 52, 154–160 (2014). https://doi.org/10.1007/s12275-014-3022-7

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  • DOI: https://doi.org/10.1007/s12275-014-3022-7

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