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Molecular characteristics and resistant mechanisms of imipenem-resistant Acinetobacter baumannii isolates in Shenyang, China

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Abstract

The investigation was carried out to elucidate the molecular characteristics and resistant mechanisms of imipenem-resistant Acinetobacter baumannii. Thirty-seven isolates were collected from January 2007 to December 2007. The homology of the isolates was analyzed by both pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). The genes of β-lactamases, adeB, and class 1 integron were polymerase chain reaction amplified. Genotype analysis of the 37 A. baumannii isolates by PFGE revealed the circulation of four PFGE types (A-D); the A- and B-type accounted for 48.6% and 40.5%, respectively. MLST showed the existence of three allelic profiles. The agar dilution method was carried out to determine the MIC of imipenem, in the absence or presence of carbonyl cyanide m-chlorophenylhydrazone (CCCP, 10 μg/ml). The MICs of the strains to imipenem were between 16 μg/ml and 128 μg/ml. When CCCP was added, a MIC decrease of at least four-fold was observed in 20 isolates, which belonged to the A- or C-type. AdeB and bla PER-1 genes were each detected in 35 isolates, bla OXA-23 gene in 34 isolates and bla OXA-58-like gene in 24 isolates. All isolates harbored bla OXA-51-like genes. No isolates carried the bla IMP-1 gene. Integron was detected in 25 isolates, which mediated the resistance to aminoglycosides and rifampin. The epidemiologic data suggested that the increasing infection of A. baumannii in our hospital was mainly caused by the inter-hospital spread of two epidemic clones. The AdeABC efflux system may be the important factor that leads to the high level of imipenem-resistance in PFGE A-type.

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Authors and Affiliations

  1. Division of Infectious Diseases, The First Hospital of China Medical University, Shenyang, 110001, P. R. China

    Jing Ping Zhang & Bai Yi Chen

  2. Department of Nosocomial Infection Control, The First Hospital of China Medical University, Shenyang, 110001, P. R. China

    Wan Zhu

  3. Department of Laboratory Medicine, The First Hospital of China Medical University, Shenyang, 110001, P. R. China

    Su Fei Tian & Yun Zhuo Chu

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  1. Jing Ping Zhang
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Zhang, J.P., Zhu, W., Tian, S.F. et al. Molecular characteristics and resistant mechanisms of imipenem-resistant Acinetobacter baumannii isolates in Shenyang, China. J Microbiol. 48, 689–694 (2010). https://doi.org/10.1007/s12275-010-0137-3

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