Abstract
To investigate the inhibitory effects of Ginsenoside Rb1 (GRb1) on apoptosis caused by Herpes Simplex Virus-1(HSV-1) in Human Glioma Cells (U251), U251 cells were infected by HSV-1 at a multiplicity of infection of 5 and GRb1, GRb1+HSV-1, HSV-1 and control groups. MTT and cell apoptosis assays were used to detect the inhibitory effects of GRb1 on the apoptosis of U251 cells that caused by HSV-1 infection for various concentrations of drug and virus treatments by MTT assay. We found that in the 400 μg/mL GRb1 and 400 μg/mL GRb1+HSV-1 groups, MTT values were higher than control group at all times (P<0. 05). Moreover, the apoptosis rate in the 400 μg/mL GRb1+HSV-1 group was lower than the HSV-1 group (P<0. 05). These results confirmed that, at appropriate concentrations, GRb1 could inhibit nerve cell apoptosis in HSV-1 infections.
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Foundation items: Supported by National Natural Science Foundation of China (Grant No. 81070501 and 30770105), and Shandong Provincial Outstanding Medical Academic Professional Program.
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Liang, YY., Wang, B., Qian, DM. et al. Inhibitory effects of Ginsenoside Rb1 on apoptosis caused by HSV-1 in Human Glioma Cells. Virol. Sin. 27, 19–25 (2012). https://doi.org/10.1007/s12250-012-3220-6
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DOI: https://doi.org/10.1007/s12250-012-3220-6