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Distribution of pathogenicity island markers in commensal and uropathogenic Escherichia coli isolates

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Abstract

Uropathogenic Escherichia coli (UPEC) isolates contain large genomic segments, termed pathogenicity islands (PAIs), that contribute to their virulence. A total of 150 UPEC and 50 commensal E. coli isolates from outpatients were investigated for antimicrobial susceptibility and the presence of eight PAI markers. One hundred ninety (95 %) isolates were resistant to one or more antimicrobial agents. The most frequent resistance found against amoxicillin (68 %), amoxicillin/clavulanic acid (55 %), aztreonam (50 %), trimethoprim/sulfamethoxazole (46 %) and tetracycline (43.5 %). Antimicrobial resistance among UPEC isolates was higher than that of commensals. PAI markers were detected in substantial percentage of commensal (88 %) and UPEC isolates (98.6 %) (P > 0.05). The most prevalent PAI marker among UPEC and commensal isolates was PAI IV536 (98.7 % UPEC vs. 84 % commensal). We found a high number of PAI markers such as PAI ICFT073, PAI IICFT073, PAI I536, PAI II536, PAI III536 and PAI IIJ96 significantly associated with UPEC. PAI III536 (21.3 %) and PAI IIJ96 (8 %) were detected only in the uropathogenic isolates. Several different combinations of PAIs were found among UPEC isolates. Comparison of PAIs among UPEC and commensal isolates showed that many UPEC isolates (79.3 %) carried two or more PAI markers, while 6 % of commensals had two PAI markers (P < 0.05). The most frequent combinations of PAI markers in UPEC isolates were PAI IV536 + PAI IICFT073 (18 %) and PAI IV536 + PAI ICFT073 + PAI IICFT073 (18 %). These results indicate that PAI markers are widespread among commensal and UPEC isolates and these commensal isolates may be reservoirs for transmission of these markers.

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References

  • Ananias M, Yano T (2008) Serogroups and virulence genotypes of Escherichia coli isolated from patients with sepsis. Braz J Med Biol L Res 41(10):877–883

    Article  CAS  Google Scholar 

  • Che D, Hasan MS, Chen B (2014) Identifying pathogenicity islands in bacterial pathogenomics using computational approaches. Pathogens 3:36–56

    Article  PubMed  PubMed Central  Google Scholar 

  • Clinical and Laboratory Standards Institute (2013) Performance standards for antimicrobial susceptibility testing; 23th informational supplement. CLSI document M100-S23. Vol. 31 No.1

    Google Scholar 

  • Copur-Cicek A, Ozgumus OB, Saral A, Sandalli C (2014) Antimicrobial resistance patterns and integron carriage of Escherichia coli isolates causing community-acquired infections in Turkey. Ann Lab Med 34:139–144

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Dobrindt U, Blum-Oehle G, Nagy G, Schneider G, Johann A, Gottschalk G (2002) Genetic structure and distribution of four pathogenicity islands (PAI I536 to PAI IV536) of uropathogenic Escherichia coli strain 536. Infect Immun 70(11):6365–6372

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Farshad S, Ranjbar R, Japoni A, Hosseini M, Anvarinejad M, Mohammadzadegan R (2012) Microbial susceptibility, virulence factors, and plasmid profiles of uropathogenic Escherichia coli strains isolated from children in Jahrom, Iran. Arch Iran Med 15(5):312–316

    PubMed  Google Scholar 

  • Gal-Mor O, Finlay BB (2006) Pathogenicity islands: a molecular toolbox for bacterial virulence. Cell Microbiol 8(11):1707–1719

    Article  CAS  PubMed  Google Scholar 

  • Hacker J, Bender L, Ott M, Wingender J, Lund B, Marre R, Goebel W (1990) Deletions of chromosomal regions coding for fimbriae and hemolysins occur in vitro and in vivo in various extraintestinal Escherichia coli isolates. Microbial pathogenesis 8(3):213–225

    Article  CAS  PubMed  Google Scholar 

  • Koga VL, Tomazetto G, Cyoia P S, Neves M S, Vidotto M C, Nakazato G, Kobayashi R K T (2014) Molecular screening of virulence genes in extraintestinal pathogenic Escherichia coli isolated from human blood culture in Brazil. Biomed Res Int Article, ID 465054; http://dx.doi.org/10.1155/2014/465054.

  • Li B, Sun JY, Han LZ, Huang XH, Fu Q, Ni YX (2010) Phylogenetic groups and pathogenicity island markers in fecal Escherichia coli isolates from asymptomatic humans in China. Appl Environ Microb 76(19):6698–6700

    Article  CAS  Google Scholar 

  • Middendorf B, Hochhut B, Leipold K, Dobrindt U, Blum-Oehler G, Hacker J (2004) Instability of pathogenicity islands in uropathogenic Escherichia coli 536. J Bacteriol 186:3086–3096

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Murugan K, Savitha T, Vasanthi S (2012) Retrospective study of antibiotic resistance among uropathogens from rural teaching hospital, Tamilnadu, India. Asian Pacific J Tropical Dis 2(5):375–380

    Article  Google Scholar 

  • Navidinia M, Najar Peerayeh S, Fallah F, Bakhshi B (2013a) Phylogenetic groups and pathogenicity island markers in Escherichia coli isolated from children. Jundishapur J Microbiol 6(10):e8362

    Article  Google Scholar 

  • Navidinia M, Najar Peerayeh S, Fallah F, Bakhshi B, Adabian S, Alimehr S, Gholinejad Z (2013b) Distribution of the pathogenicity islands markers (PAIs) in uropathogenic E.coli isolated from children in Mofid children hospital, Pediatric Infections Research Center. Arch Pediatr Infect Dis 1(2):75–79

    Article  Google Scholar 

  • Neamati F, Firoozeh F, Saffari M, Zibaei M (2015) Virulence genes and antimicrobials resistance pattern in uropathogenic Escherichia coli isolated from hospitalized patients in Kashan, Iran. Jundishapur J Microbiol 8(2):e17514

    Article  PubMed  PubMed Central  Google Scholar 

  • Oliveira FA, Paludo KS, Arend LN, Farah SM, Pedrosa FO, Souza EM, Surek M, Picheth G, Fadel-Picheth CM (2011) Virulence characteristics and antimicrobial susceptibility of uropathogenic Escherichia coli strains. Genet Mol Res 10(4):4114–4125

    Article  CAS  PubMed  Google Scholar 

  • Östblom A, Adlerberth I, Wold AE, Nowrouzian FL (2011) Pathogenicity island markers, virulence determinants malX and usp, and the capacity of Escherichia coli to persist in infants’ commensal microbiotas. Appl Environ Microb 77(7):2303–2308

    Article  Google Scholar 

  • Phongpaichit S, Tunyapanit W, Pruekprasert P (2011) Antimicrobial resistance, class I integrons and extended spectrum betalactamases in E. coli clinical isolates from patients in south Thailand. J Health Sci 57:281–288

    Article  Google Scholar 

  • Pobiega M, Wojkowska-Mach J, Chmielarczyk A, Romaniszyn D, Adamski P, Heczko PB et al (2013) Molecular characterization and drug resistance of Escherichia coli strains isolated from urine from long-term care facility residents in Cracow, Poland. Med Sci Monit 19:317–326

    Article  PubMed  PubMed Central  Google Scholar 

  • Ravi A, Avershina E, Ludvigsen J, L’Abée-Lund TM, Rudi K (2014) Integrons in the intestinal microbiota as reservoirs for transmission of antibiotic resistance genes. Pathogens 3:238–248

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Rezaee MA, Sheikhalizadeh V, Hasani A (2011) Detection of integrons among multi-drug resistant (MDR) Escherichia coli strains isolated from clinical specimens in northern west of Iran. Braz J Microbiol 42(4):1308–1313

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Rice LB (2009) The clinical consequences of antimicrobial resistance. Curr Opin Microbiol 12:476–481

    Article  CAS  PubMed  Google Scholar 

  • Sabaté M, Moreno E, Pérez T, Andreu A, Prats G (2006) Pathogenicity island markers in commensal and uropathogenic Escherichia coli isolates. Clin Microbiol Infect 12:880–886

    Article  PubMed  Google Scholar 

  • Schmidt H, Hensel M (2004) Pathogenicity islands in bacterial pathogenesis. Clin Microbiol Rev 17(1):14–56

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Sepp E, Stsepetova J, Lõivukene K, Truusalu K, Kõljalg S, Naaber P, Mikelsaar M (2009) The occurrence of antimicrobial resistance and class 1 integrons among commensal Escherichia coli isolates from infants and elderly persons. Annals of Clinical Microbiology and Antimicrobials 8:34

    Article  PubMed  PubMed Central  Google Scholar 

  • Su J, Shi L, Yang L, Xiao Z, Li X, Yamasaki S (2006) Analysis of integrons in clinical isolates of Escherichia coli in China during the last six years. FEMS Microbiol Lett 254:75–80

    Article  CAS  PubMed  Google Scholar 

  • Wenting J, Jinling S, Magaly T, Shaohua Z, Jianghong M (2013) Distribution of pathogenicity islands OI-122, OI-43/48, and OI-57 and a high-pathogenicity island in shiga toxin-producing Escherichia coli. Appl Environ Microb 79(11):3406–3412

    Article  Google Scholar 

  • Wozniak RAF, Waldor MK (2010) Integrative and conjugative elements: mosaic mobile genetic elements enabling dynamic lateral gene flow. Nat Rev Microbiol 8(8):552–563

    Article  CAS  PubMed  Google Scholar 

  • Yoon SH, Hur CG, Kang HY, Kim YH, Oh TK, Kim JF (2005) A computational approach for identifying pathogenicity islands in prokaryotic genomes. BMC Bioinformatics 6:184

    Article  PubMed  PubMed Central  Google Scholar 

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Authors and Affiliations

  1. Department of Microbiology, Zanjan University of Medical Sciences, Zanjan, Iran

    Ali Samei, Fakhri Haghi & Habib Zeighami

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  1. Ali Samei
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  2. Fakhri Haghi
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  3. Habib Zeighami
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Correspondence to Habib Zeighami.

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Samei, A., Haghi, F. & Zeighami, H. Distribution of pathogenicity island markers in commensal and uropathogenic Escherichia coli isolates. Folia Microbiol 61, 261–268 (2016). https://doi.org/10.1007/s12223-015-0433-8

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