Skip to main content

Advertisement

SpringerLink
Log in

A Novel Ribonuclease with HIV-1 Reverse Transcriptase Inhibitory Activity from the Edible Mushroom Hygrophorus russula

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

A 28-kDa ribonuclease, with an optimum pH of 4.0 and an optimum temperature at 58 °C, was isolated from fruiting bodies of the edible mushroom Hygrophorus russula. It was purified by ion exchange chromatography on carboxymethyl-cellulose, dithyaminoethyl-cellulose, quaternary amine-sepharose and sulphopropyl-sepharose, followed by fast protein liquid chromatography gel filtration on Superdex 75. The N-terminal amino acid sequence was ASAGG which showed homology to those of other fungal RNases to some degree. It exerted the highest RNase activity on poly C and poly U. The Michaelis constant (K m) value of the RNase on yeast tRNA was 3.6 μM, and the maximal velocity (V max) was 0.6 μM. The RNase activity was suppressed by some ions including Fe2+ and Zn2+ ions. The RNase inhibited the activity of HIV-1 reverse transcriptase with an IC50 of 4.64 μM.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Norioka, S., Oneyama, C., Takuma, S., Shinkawa, T., Ishimizu, T., Nakanishi, T., et al. (2007). Purification and characterization of a non-S-RNase and S-RNases from styles of Japanese pear (Pyrus pyrifolia). Plant Physiology and Biochemistry, 45(12), 878–886.

    Article  CAS  Google Scholar 

  2. Schein, A., Sheffy-Levin, S., Glaser, F., & Schuster, G. (2008). The RNase E/G-type endoribonuclease of higher plants is located in the chloroplast and cleaves RNA similarly to the E. coli enzyme. RNA, 14(6), 1057–1068.

    Article  CAS  Google Scholar 

  3. Tanaka, N., Arai, J., Inokuchi, N., Koyama, T., Ohgi, K., Irie, M., et al. (2000). Crystal structure of a plant ribonuclease, RNase LE. Journal of Molecular Biology, 298(5), 859–873.

    Article  CAS  Google Scholar 

  4. Fang, E. F., & Ng, T. B. (2011). Ribonucleases of different origins with a wide spectrum of medicinal applications. Biochimica et Biophysica Acta, 1815(1), 65–74.

    CAS  Google Scholar 

  5. Newton, D. L., Stockwin, L. H., & Rybak, S. M. (2009). Anti-CD22 onconase: preparation and characterization. Methods Mol Biol, 525, 425–43. xiv.

    Article  CAS  Google Scholar 

  6. Mamolen, M., & Andrulis, E. D. (2009). Characterization of the Drosophila melanogaster Dis3 ribonuclease. Biochemical and Biophysical Research Communications, 390(3), 529–534.

    Article  CAS  Google Scholar 

  7. Vourekas, A., Vryzaki, E., Toumpeki, C., Stamatopoulou, V., Monastirli, A., Tsambaos, D., et al. (2009). Partial purification and characterization of RNase P from human peripheral lymphocytes. Experimental Dermatology, 18(2), 130–133.

    Article  CAS  Google Scholar 

  8. Aphanasenko, G. A., Dudkin, S. M., Kaminir, L. B., Leshchinskaya, I. B., & Severin, E. S. (1979). Primary structure of ribonuclease from Bacillus intermedius 7P. FEBS Letters, 97(1), 77–80.

    Article  CAS  Google Scholar 

  9. Sana, B., Ghosh, D., Saha, M., & Mukherjee, J. (2008). Purification and characterization of an extracellular, uracil specific ribonuclease from a Bizionia species isolated from the marine environment of the Sundarbans. Microbiology Research, 163(1), 31–38.

    Article  CAS  Google Scholar 

  10. Hlinkova, V., Urbanikova, L., Krajcikova, D., & Sevcik, J. (2001). Purification, crystallization and preliminary X-ray analysis of two crystal forms of ribonuclease Sa3. Acta Crystallographica Section D: Biological Crystallography, 57(Pt 5), 737–739.

    Article  CAS  Google Scholar 

  11. Kobayashi, H., Inokuchi, N., Koyama, T., Watanabe, H., Iwama, M., Ohgi, K., et al. (1992). Primary structure of a base non-specific and adenylic acid preferential ribonuclease from the fruit bodies of Lentinus edodes. Bioscience, Biotechnology, and Biochemistry, 56(12), 2003–2010.

    Article  CAS  Google Scholar 

  12. Wang, H., & Ng, T. B. (1999). Isolation of a new ribonuclease from fresh fruiting bodies of the straw mushroom. Biochemical and Biophysical Research Communications, 264(3), 714–718.

    Article  CAS  Google Scholar 

  13. Ngai, P. H., Wang, H. X., & Ng, T. B. (2003). Purification and characterization of a ubiquitin-like peptide with macrophage stimulating, antiproliferative and ribonuclease activities from the mushroom Agrocybe cylindracea. Peptides, 24(5), 639–645.

    Article  CAS  Google Scholar 

  14. Zhang, R. Y., Zhang, G. Q., Hu, D. D., Wang, H. X., & Ng, T. B. (2010). A novel ribonuclease with antiproliferative activity from fresh fruiting bodies of the edible mushroom Lyophyllum shimeiji. Biochemical Genetics, 48(7-8), 658–668.

    Article  CAS  Google Scholar 

  15. Ercole, C., Spadaccini, R., Alfano, C., Tancredi, T., & Picone, D. (2007). A new mutant of bovine seminal ribonuclease with a reversed swapping propensity. Biochemistry, 46(8), 2227–2232.

    Article  CAS  Google Scholar 

  16. Zhao, S., Zhao, Y., Li, S., Zhang, G., Wang, H., & Ng, T. B. (2010). An antiproliferative ribonuclease from fruiting bodies of the wild mushroom Russula delica. Journal of Microbiology and Biotechnology, 20(4), 693–699.

    Article  CAS  Google Scholar 

  17. Guan, G. P., Wang, H. X., & Ng, T. B. (2007). A novel ribonuclease with antiproliferative activity from fresh fruiting bodies of the edible mushroom Hypsizigus marmoreus. Biochimica et Biophysica Acta, 1770(12), 1593–1597.

    Article  CAS  Google Scholar 

  18. Wang, H. X., & Ng, T. B. (2001). Purification and characterization of a potent homodimeric guanine-specific ribonuclease from fresh mushroom (Pleurotus tuber-regium) sclerotia. The International Journal of Biochemistry and Cell Biology, 33(5), 483–490.

    Article  CAS  Google Scholar 

  19. Wang, H., & Ng, T. B. (2003). A novel ribonuclease from the veiled lady mushroom Dictyophora indusiata. Biochemical Cell Biology, 81(6), 373–377.

    Article  CAS  Google Scholar 

  20. Lam, Y. W., & Ng, T. B. (2001). A monomeric mannose-binding lectin from inner shoots of the edible chive (Allium tuberosum). Journal of Protein Chemistry, 20(5), 361–366.

    Article  CAS  Google Scholar 

  21. Zhao, J. K., Wang, H. X., & Ng, T. B. (2009). Purification and characterization of a novel lectin from the toxic wild mushroom Inocybe umbrinella. Toxicon, 53(3), 360–366.

    Article  CAS  Google Scholar 

  22. Zhao, Y. C., Zhang, G. Q., Ng, T. B., & Wang, H. X. (2011). A novel ribonuclease with potent HIV-1 reverse transcriptase inhibitory activity from cultured mushroom Schizophyllum commune. Journal of Microbiology, 49(5), 803–808.

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This work was financially supported by National Grants of China (Biomass dissociation and low-molecular fragment green monomerization and transformation, 2010CB732202).

Author information

Authors and Affiliations

  1. State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University, Beijing, 100193, China

    Mengjuan Zhu, Lijing Xu, Xiao Chen, Zengqiang Ma & Hexiang Wang

  2. School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China

    T. B. Ng

Authors
  1. Mengjuan Zhu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Lijing Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Xiao Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Zengqiang Ma
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Hexiang Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. T. B. Ng
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Hexiang Wang or T. B. Ng.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhu, M., Xu, L., Chen, X. et al. A Novel Ribonuclease with HIV-1 Reverse Transcriptase Inhibitory Activity from the Edible Mushroom Hygrophorus russula . Appl Biochem Biotechnol 170, 219–230 (2013). https://doi.org/10.1007/s12010-013-0180-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12010-013-0180-8

Keywords

Search

Navigation