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Phenotypic and physiologic variability in nasal epithelium cultured from smokers and non-smokers exposed to secondhand tobacco smoke

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Abstract

The emergence of air-liquid interface (ALI) culturing of mammalian airway epithelium is a recent innovation for experimental modeling of airway epithelial development, function, and pathogenic mechanisms associated with infectious agent and irritant exposure. This construct provides an experimental platform for in vitro propagation, manipulation, and testing of airway epithelium in a structural and physiologic state that emulates in vivo organization. In this study, we have cultured nasal epithelial biopsies from human subjects with variable histories of tobacco smoke exposure and assessed ciliary beat frequency (CBF) after an extended interval in vitro relative to CBF determined on biopsies from the same subjects immediately upon acquisition. We observed elevated CBF in nasal epithelial biopsies as well as persistence of accelerated CBF in ALI cultures deriving from biopsies of smokers and non-smokers exposed to environmental tobacco smoke compared to CBF in cultures from biopsies of well-documented non-smokers. Moreover, cultures deriving from smokers exhibited reduced ciliation as the cultures matured. These studies document that nasal epithelium cultured in the ALI system retains physiologic and phenotypic characteristics of the epithelial layer in vivo even through rounds of proliferative expansion. These observations suggest that stable epigenetic factors affecting regulation of ciliary function and phenotype commitment may be operative.

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Acknowledgments

This study was supported by a Clinical Innovator Award to JLC from the Flight Attendant Medical Research Institute, Grant #R01CA79949 from the National Institutes of Health to HZ, and the US Environmental Protection Agency. Although the research described in this article has been funded wholly or in part by the US Environmental Protection Agency through cooperative agreement CR833463-01 with the Center for Environmental Medicine, Asthma, and Lung Biology at the University of North Carolina at Chapel Hill, it has not been subjected to the agency’s required peer and policy review, and therefore does not necessarily reflect the views of the agency and no official endorsement should be inferred. Mention of trade names or commercial products does not constitute endorsement or recommendation for use.

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Authors and Affiliations

  1. Department of Biostatistics, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA

    Haibo Zhou

  2. Department of Pediatrics, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA

    Johnny L. Carson & Ilona Jaspers

  3. Center for Environmental Medicine, Asthma, and Lung Biology, The University of North Carolina at Chapel Hill, CB #7310, 104 Mason Farm Road, Chapel Hill, NC, 27599-7310, USA

    Johnny L. Carson, Luisa Brighton, Milan Hazucha, Ilona Jaspers & Haibo Zhou

  4. The Department of Mathematics, National Taiwan Normal University, Taipei, Taiwan, Republic of China

    Tsui-Shan Lu

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  1. Johnny L. Carson
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  2. Tsui-Shan Lu
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  3. Luisa Brighton
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  4. Milan Hazucha
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  5. Ilona Jaspers
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  6. Haibo Zhou
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Correspondence to Johnny L. Carson.

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Editor: J. Denry Sato

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Carson, J.L., Lu, TS., Brighton, L. et al. Phenotypic and physiologic variability in nasal epithelium cultured from smokers and non-smokers exposed to secondhand tobacco smoke. In Vitro Cell.Dev.Biol.-Animal 46, 606–612 (2010). https://doi.org/10.1007/s11626-010-9310-6

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  • DOI: https://doi.org/10.1007/s11626-010-9310-6

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