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Influences of graphene oxide on biofilm formation of gram-negative and gram-positive bacteria

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Abstract

In this study, we evaluated the influences of graphene oxide (GO) on biofilm formation. Escherichia coli MG1655 and Bacillus subtilis 168 were used as models for Gram-negative and Gram-positive bacteria. The growth profiles and viability assays indicated that GO exhibited a high antibacterial activity, of which the negative effects on bacteria growth raised with the increasing GO concentration. The antibacterial activity of GO was mainly attributed to the membrane stress and ROS-independent oxidative stress. Moreover, it was worthy to note that the biofilm formation was enhanced in the presence of GO at low dosage whereas inhibited in the high-concentration GO environment. These results could be explained by the roles of the dead cells, which were inactivated by GO. When the concentration of GO was limited, only a part of the cells would be inactivated, which may then serve as a protection barrier as well as the necessary nutrient to the remaining living cells for the formation of biofilm. In contrast, with a sufficient presence of GO, almost all cells can be inactivated completely and thus the formation of biofilm could no longer be triggered. Overall, the present work provides significant new insights on the influence of carbon nanomaterials towards biofilm formation, which has far-reaching implications in the field of biofouling and membrane bioreactor.

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Funding

The research was supported by the National Natural Science Foundation of China (21676161, 21476130 and 51208283).

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Authors and Affiliations

  1. Shandong Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Shandong University, Jinan, 250100, China

    Chao Song, Chun-Miao Yang, Xue-Fei Sun, Peng-Fei Xia, Jing Qin, Bei-Bei Guo & Shu-Guang Wang

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  1. Chao Song
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  2. Chun-Miao Yang
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  3. Xue-Fei Sun
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  4. Peng-Fei Xia
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  5. Jing Qin
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  6. Bei-Bei Guo
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  7. Shu-Guang Wang
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Correspondence to Xue-Fei Sun or Shu-Guang Wang.

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Responsible editor: Diane Purchase

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Song, C., Yang, CM., Sun, XF. et al. Influences of graphene oxide on biofilm formation of gram-negative and gram-positive bacteria. Environ Sci Pollut Res 25, 2853–2860 (2018). https://doi.org/10.1007/s11356-017-0616-8

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  • DOI: https://doi.org/10.1007/s11356-017-0616-8

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