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Fermentation of ginseng extracts by Penicillium simplicissimum GS33 and anti-ovarian cancer activity of fermented products

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Abstract

A total of 58 isolates of β-glucosidase-producing microorganisms were isolated from soil around the wild ginseng roots under forest using Esculin-R2A agar. Among these isolates, strain GS33 showed a strong ability to convert ginsenosides Rb1, Rb2, Rc, and Rd into F2, Rg3, C-K, and convert ginsenoside Rg1 into Rh1, and F1. Fermented ginseng products can inhibit ES-2 cells growth and the IC50 value was 0.73 mg ml−1. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain GS33 belongs to the genus Penicillium and is most closely related to Penicillium simplicissimum (99 %).

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Acknowledgments

This work supported by Doctoral Fund of the Ministry of Education of China (No. 20112201110001) and the National Natural Science Foundation of China (No. 20862017).

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Authors and Affiliations

  1. Department of Chemistry, Chemical Biology Research Center, Yanbian University, Gongyuan Road, Yanji, 133002, China

    Yu Fu & Chengri Yin

  2. Department of Environmental Engineering, Chungnam National University, Daejeon City, 305-764, Korea

    Zhenhao Yin

  3. National Ginseng Products Quality Supervision Inspection Center, Yanji, 133000, Jilin, China

    Lunpeng Wu

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  1. Yu Fu
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  2. Zhenhao Yin
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  3. Lunpeng Wu
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  4. Chengri Yin
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Correspondence to Chengri Yin.

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Fu, Y., Yin, Z., Wu, L. et al. Fermentation of ginseng extracts by Penicillium simplicissimum GS33 and anti-ovarian cancer activity of fermented products. World J Microbiol Biotechnol 30, 1019–1025 (2014). https://doi.org/10.1007/s11274-013-1520-0

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  • DOI: https://doi.org/10.1007/s11274-013-1520-0

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