Skip to main content
SpringerLink
Log in

Acute phase response in lame cattle with interdigital dermatitis

  • Published:
World Journal of Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

This study was undertaken to evaluate acute phase response via assessing the concentration of serum sialic acids (total, lipid-bound and protein-bound), inflammatory mediators (IFN-γ and TNF-α) and acute phase proteins [haptoglobin (Hp) and serum amyloid A (SAA)] in lame cattle with interdigital dermatitis. Fifteen hoof scrapings from lame cows were collected from eight commercial dairy farms. As a consequence of the difficulty in culturing and isolation, a PCR technique was used to detect the organism. None of the colonies on enriched blood agar was identified as Fusobacterium necrophorum. Four (26.6%) out of the 15 hoof scrapings examined tested positive for the presence of the lktA gene (402 bp) of F. necrophorum. It seems that culture cannot be considered as the gold standard method for F. necrophorum isolation. Molecular detection is suggested as an alternative method. In the blood serum of different groups of animals (control, lameness and F. necrophorum-positive lameness) Hp, SAA, total sialic acid, lipid-bound sialic acid, and protein-bound sialic acid, and IFN-γ and TNF-α were measured using validated standard procedures. All parameters were significantly higher in the lameness group and the F. necrophorum-positive lameness group compared with the healthy group (P < 0.01 in all cases). Mean SAA concentrations in the lameness group and the F. necrophorum-positive lameness group was relatively 4.6 and 8.0 times higher than the control group. Corresponding measures for Hp indicate a 3.3 times increase in the lameness group compared to the control. In the lameness group, significant associations were observed for Hp with PBSA, SAA with TSA, TSA with PBSA, TSA with LBSA, PBSA with LBSA, and SAA with IFN-γ.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  • Alsemgeest SPM, Kalsbeek HC, Wensing T, Koeman JP, Van Ederen AM, Gruys E (1994) Concentrations of serum amyloid A (SAA) and haptoglobin (Hp) as parameters of inflammatory diseases in cattle. Vet Q 16:21–23

    Article  CAS  Google Scholar 

  • Bennett G, Hickford J, Sedcole R, Zhou H (2009a) Dichelobacter nodosus, Fusobacterium necropuorum and the epidemiology of footrot. Anaerobe 15:173–176

    Article  CAS  Google Scholar 

  • Bennett G, Hickford J, Zhou H, Laporte J, Gibbs J (2009b) Detection of Fusobacterium necrophorum and Dichelobacter nodosus in lame cattle on dairy farms in New Zealand. Res Vet Sci 87:413–415

    Article  CAS  Google Scholar 

  • Brown DJ, Campbell JDM, Russell GC, Hopkins J, Glass EJ (1995) T cell activation by Theileria annulata-infected macrophages correlates with cytokine production. Clin Exp Immunol 102:507–514

    Article  CAS  Google Scholar 

  • Campbell JDM, Brown DJ, Nichani AK, Howie SEM, Spooner RL, Glass EJ (1997) A non-protective T helper1 response against the intra-macrophage protozoan Theileria annulata. Clin Exp Immunol 108:463–470

    Article  CAS  Google Scholar 

  • Citil M, Gunes V, Karapehlivan M, Atalan G, Marasli S (2004) Evaluation of serum sialic acid as an inflammation marker in cattle with traumatic reticuloperitonitis. Rev Med Vet 155:389–392

    CAS  Google Scholar 

  • Coyle-Dennis JE, Lauerman LH (1979) Correlations between leukocidin production and virulence of isolates of Fusobacterium necrophorum. Am J Vet Res 40:274–276

    CAS  Google Scholar 

  • Crook M (1993) The determination of plasma or serum sialic acid. Clin Biochem 26:31–38

    Article  CAS  Google Scholar 

  • Eckersall PD (2000) Recent advances and future prospects for the use of acute phase proteins as markers of disease in animals. Rev Med Vet 151:577–584

    CAS  Google Scholar 

  • Eckersall PD (2004) The time is right for acute phase protein assays. Vet J 168:3–5

    CAS  Google Scholar 

  • Ekin S, Mert N, Gunduz H, Meral I (2003) Serum sialic acid levels and selected mineral status in patients with type 2 diabetes mellitus. Biol Trace Elem Res 94:193–201

    Article  CAS  Google Scholar 

  • Enjuanes L, Spaan WJ, Snijder EJ, Cavanagh D (2000) Nidovirales. In: Regenmortel MHV, Fauquet CM, Bishop DHL, Carsten EB, Estes MK, Lemon SM, McGeoch DJ, Maniloff J, Mayo MA, Pringle CR, Wickner RB (eds) Virus taxonomy. Classification and nomenclature of viruses. Academic Press, New York, pp 827–834

    Google Scholar 

  • Ertekin A, Keles I, Ekin S, Karaca M, Akkan HA (2000) An investigation on sialic acid and lipid-bounded sialic acid levels in animals with blood parasites. J Fac Vet Med Univ Yüzüncü Yil 11:34–35

    Google Scholar 

  • Ganheim C, Hulten C, Carlsson U, Kindahl H, Niskanen R, Waller KP (2003) The acute phase response in calves experimentally infected with bovine viral diarrhea virus and/or Mannheimia heamolytica. J Vet Med B 50:183–190

    Article  CAS  Google Scholar 

  • Gruys E, Toussaint MJM, Niewold TA, Koopmans SJ (2005) Acute phase reaction and acute phase proteins. J Zhejiang Univ Sci 6:1045–1056

    Article  CAS  Google Scholar 

  • Gunes V, Karapehlivan M, Citil M, Atalan G, Marasli S (2004) Relationship between serum sialic acid levels and eye lesions in calves with infectious bovine keratoconjunctivitis. Rev Med Vet 155:508–510

    CAS  Google Scholar 

  • Haq M, Haq S, Tutt P, Crook M (1993) Serum total sialic acid and lipid-associated sialic acid in normal individuals and patients with myocardial infarction and their relationship to acute phase proteins. Ann Clin Biochem 30:383–386

    Google Scholar 

  • Hernandez J, Shearer JK, Webb DW (2001) Effect of lameness on the calving to conception interval in dairy cows. J Am Vet Med Assoc 218:1611–1614

    Article  CAS  Google Scholar 

  • Hurtado MA, Piriz S, Valle J, Jimenez R, Vadillo S (1998) Etiology of ovine footrot in Spain. Vet Rec 142:60–63

    Article  CAS  Google Scholar 

  • Jawor P, Steiner S, Stefaniak T, Baumgartner W, Rzasa A (2008) Determination of selected acute phase proteins during the treatment of limb diseases in dairy cows. Vet Med 53:173–183

    CAS  Google Scholar 

  • Karagenc TI, Kiral FK, Seyrek K, Bildik A, Eren H (2005) Detection of serum total sialic acid in cattle with natural tropical theileriosis. Rev Med Vet 156:578–582

    CAS  Google Scholar 

  • Karapehlivan M, Atakisi E, Citil M, Kankavi O, Atakisi O (2007) Serum sialic acid levels in calves with pneumonia. Vet Res Commun 31:37–41

    Article  CAS  Google Scholar 

  • Katoh N, Nakagawa H (1999) Detection of haptoglobin in the high-density lipoprotein fractions from sera of calves with experimental pneumonia and cows with naturally occurring fatty liver. J Vet Med Sci 61:119–124

    Article  CAS  Google Scholar 

  • Katopodis N, Hirshaut Y, Geller NL, Stock CC (1982) Lipid-associated sialic acid test for the detection of human cancer. Cancer Res 42:5270–5275

    CAS  Google Scholar 

  • Keles I, Ertekin A, Karaca M, Ekin S, Akkan HA (2000) An investigation on serum sialic acid and lipid-bounded sialic acid levels in cattle with leptospirosis. J Fac Vet Med Univ Yüzüncü Yil 11:121–122

    Google Scholar 

  • King DJ, Cavanagh D (1991) Infectious bronchitis. In: Calnek BW, Barnes HJ, Reid WM, Yoder HW (eds) Diseases of poultry, 9th edn. Iowa State University Press, Ames, pp 471–484

    Google Scholar 

  • Makimura S, Usui M (1990) Correlation between haptoglobin and sialic acid or mucoprotein in diseased bovine serum. Jpn J Vet Sci 52:1245–1250

    Article  CAS  Google Scholar 

  • Murata H (2007) Stress and acute phase protein response: an inconspicuous but essential linkage. Vet J 173:473–474

    Article  Google Scholar 

  • Murata H, Shimada N, Yoshioka M (2004) Current research on acute phase proteins in veterinary diagnosis: an overview. Vet J 168:28–40

    Article  CAS  Google Scholar 

  • Nagaraja TG, Narayanan SK, Stewart GC, Chengappa MM (2005) Fusobacterium necrophorum infections in animals: pathogenesis and pathogenic mechanisms. Anaerobe 11:239–246

    Article  CAS  Google Scholar 

  • Nazifi S, Khoshvaghti A, Gheisari HR (2008a) Evaluation of serum and milk amyloid A in some inflammatory diseases of cattle. Iran J Vet Res 9:222–226

    Google Scholar 

  • Nazifi S, Rezakhani A, Koohimoghadam M, Ansari-Lari M, Esmailnezhad Z (2008b) Evaluation of serum haptoglobin in clinically healthy cattle and cattle with inflammatory diseases in Shiraz, a tropical area in southern Iran. Bulgarian J Vet Med 11:95–101

    Google Scholar 

  • Oelke AM, Nagaraja TG, Wilkerson MJ, Stewart GC (2005) The leukotoxin operon of Fusobacterium necrophorum is not present in other species of Fusobacterium. Anaerobe 11:123–129

    Article  CAS  Google Scholar 

  • Petersen HH, Nielsen JP, Heegaard PMH (2004) Application of acute phase protein measurements in veterinary clinical chemistry. Vet Res 35:163–187

    Article  CAS  Google Scholar 

  • Quinn PJ, Carter ME, Markey B, Carter GR (1994) Clinical veterinary microbiology. Mosby Co., St. Louis

    Google Scholar 

  • Smith BI, Kauffold J, Sherman L (2010) Serum haptoglobin concentrations in dairy cattle with lameness due to claw disorders. Vet J 186:162–165

    Article  CAS  Google Scholar 

  • Sonel B, Tutkak H, Düzgün N (2002) Serum levels of IL-1beta, TNF-alpha, IL-8, and acute phase proteins in seronegative spondyloarthropathies. Joint Bone Spine 69:463–467

    Article  Google Scholar 

  • Thougaard AV, Hellmen E, Jensen AL (1998) Total serum sialic acid is a general disease marker rather than a specific tumor marker in dogs. J Vet Med A 45:471–479

    Article  CAS  Google Scholar 

  • Warnick LD, Janssen D, Guard CL, Grohn YT (2001) The effect of lameness on milk production in dairy cows. J Dairy Sci 84:1988–1997

    Article  CAS  Google Scholar 

  • Warren L (1959) The thiobarbituric acid assay of sialic acids. J Biol Chem 234:1971

    CAS  Google Scholar 

  • Zhou H, Hickford JGH (2000) Extensive diversity in New Zealand Dichelobacter nodosus strains from infected sheep and goats. Vet Microb 71:113–123

    Article  CAS  Google Scholar 

  • Zhou H, Grant Bennett G, Hickford JGH (2009) Variation in Fusobacterium necrophorum strains present on the hooves of footrot infected sheep, goats and cattle. Vet Microbiol 135:363–367

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors would like to thank the members of the Research Committee of Shiraz University for providing financial support.

Author information

Authors and Affiliations

  1. Department of Clinical Studies, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

    S. Nazifi, S. Ghadirian & A. Mirzaei

  2. Department of Pathobiology, School of Veterinary Medicine, Shiraz University, P.O. Box: 1731, 71345, Shiraz, Iran

    Z. Esmailnezhad & M. Haghkhah

Authors
  1. S. Nazifi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Z. Esmailnezhad
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Haghkhah
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. S. Ghadirian
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. A. Mirzaei
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to S. Nazifi.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Nazifi, S., Esmailnezhad, Z., Haghkhah, M. et al. Acute phase response in lame cattle with interdigital dermatitis. World J Microbiol Biotechnol 28, 1791–1796 (2012). https://doi.org/10.1007/s11274-011-0995-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11274-011-0995-9

Keywords

Search

Navigation