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Discovery and molecular characterization of a novel enamovirus, Grapevine enamovirus-1

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Abstract

In this study, we describe a novel putative Enamovirus member, Grapevine enamovirus-1 (GEV-1), discovered by high-throughput sequencing (HTS). A limited survey using HTS of 17 grapevines (Vitis spp.) from the south, southeast, and northeast regions of Brazil led to the detection of GEV-1 exclusively on southern plants, infecting four grapevine cultivars (Cabernet Sauvignon, Semillon, CG 90450, and Cabernet franc) with a remarkable identity of around 99% at the nucleotide level. This novel virus was only detected in multiple-virus infected plants exhibiting viral-like symptoms. GEV-1 was also detected on a cv. Malvasia Longa by RT-PCR. We performed graft-transmissibility assays on GEV-1. The organization, products, and cis-acting regulatory elements of GEV-1 genome are also discussed here. The near complete genome sequence of GEV-1 was obtained during the course of this study, lacking only part of the 3′ untranslated terminal region. This is the first report of a virus in the family Luteoviridae infecting grapevines. Based on its genomic properties and phylogenetic analyses, GEV-1 should be classified as a new member of the genus Enamovirus.

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Acknowledgements

This study was funded by Embrapa (Empresa Brasileira de Pesquisa Agropecuária, Brazil), Project 02.13.14.002. Scholarship of JMFS was supported by CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brazil). We thank Osmar Nickel for his kind review of the manuscript.

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Authors and Affiliations

  1. University of Brasília, Brasília, DF, Brazil

    João Marcos Fagundes Silva, Rosana Blawid & Tatsuya Nagata

  2. Department of Plant Pathology, University of California, Davis, CA, USA

    Maher Al Rwahnih

  3. Embrapa Grape & Wine, Bento Gonçalves, RS, Brazil

    Thor Vinícius Martins Fajardo

Authors
  1. João Marcos Fagundes Silva
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  2. Maher Al Rwahnih
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  3. Rosana Blawid
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  4. Tatsuya Nagata
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  5. Thor Vinícius Martins Fajardo
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Contributions

TVMF designed and supervised the study, and performed initial confirmation of infection and graft-transmissibility assays. MAR performed de novo assemblies and BLASTX searches, as well as primer design. Further bioinformatics analysis and 5′ RACE were performed by JMFS and RB. TN supervised the study. JMFS wrote the manuscript, and all authors contributed to the review.

Corresponding author

Correspondence to Thor Vinícius Martins Fajardo.

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The authors declare that they have no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Edited by Thomas Hohn.

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Silva, J.M.F., Al Rwahnih, M., Blawid, R. et al. Discovery and molecular characterization of a novel enamovirus, Grapevine enamovirus-1. Virus Genes 53, 667–671 (2017). https://doi.org/10.1007/s11262-017-1470-y

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  • DOI: https://doi.org/10.1007/s11262-017-1470-y

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