Skip to main content
SpringerLink
Log in

The p19.7 RNA silencing suppressor from Grapevine leafroll-associated virus 3 shows different levels of activity across phylogenetic groups

  • Published:
Virus Genes Aims and scope Submit manuscript

Abstract

At least five phylogenetic groups have been reported for Grapevine leafroll-associated virus 3 (GLRaV-3). The p19.7 protein encoded by the GLRaV-3 was previously identified as an RNA silencing suppressor. In this study, five constructs of p19.7 belonging to different groups were compared for their suppressing activity. For each p19.7 variant, the accumulation level of green fluorescent protein mRNA and specific siRNAs were determined using co-infiltration assays in transgenic 16C Nicotiana benthamiana. Differences in the suppressing activity were found among the variants assayed. Some constructs originated viral-like mosaic symptoms that evolved to necrosis. The intensity of these symptoms appeared to be related to the strength of the suppressor activity. A comparison of the protein sequences revealed a few amino acid substitutions that may be associated with the observed differences in the suppressing activity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. D. Baulcombe, Nature 431, 356–363 (2004)

    Article  PubMed  CAS  Google Scholar 

  2. P. Brodersen, O. Voinnet, Trends Genet. 22, 268–280 (2006)

    Article  PubMed  CAS  Google Scholar 

  3. S.W. Ding, O. Voinnet, Cell 130, 413–426 (2007)

    Article  PubMed  CAS  Google Scholar 

  4. E.J. Chapman, A.I. Prokhnevsky, K. Gopinath, V.V. Dolja, J.C. Carrington, Genes Dev. 18, 1179–1186 (2004)

    Article  PubMed  CAS  Google Scholar 

  5. P. Dunoyer, C.H. Lecellier, E.A. Parizotto, C. Himber, O. Voinnet, Plant Cell 16, 1235–1250 (2004)

    Article  PubMed  CAS  Google Scholar 

  6. V. Pallas, J.A. Garcia, J. Gen. Virol. 92, 2691–2705 (2011)

    Article  PubMed  CAS  Google Scholar 

  7. F. Li, S.W. Ding, Annu. Rev. Microbiol. 60, 503–531 (2006)

    Article  PubMed  CAS  Google Scholar 

  8. O. Voinnet, Nat. Rev. Genet. 6, 206–220 (2005)

    Article  PubMed  CAS  Google Scholar 

  9. J.A. Diaz-Pendon, S.W. Ding, Annu. Rev. Phytopathol. 46, 303–326 (2008)

    Article  PubMed  CAS  Google Scholar 

  10. T. Mangwende, M.L. Wang, W. Borth, J. Hu, P.H. Moore, T.E. Mirkov, H.H. Albert, Virology 384, 38–50 (2009)

    Article  PubMed  CAS  Google Scholar 

  11. H. Senshu, J. Ozeki, K. Komatsu, M. Hashimoto, K. Hatada, M. Aoyama, S. Kagiwada, Y. Yamaji, S. Namba, J. Gen. Virol. 90, 1014–1024 (2009)

    Article  PubMed  CAS  Google Scholar 

  12. W.J. Cuellar, F. Tairo, J.F. Kreuze, J.P.T. Valkonen, J. Gen. Virol. 89, 573–582 (2008)

    Article  PubMed  CAS  Google Scholar 

  13. N.T. Marques, A.A. Costa, D. Lopes, G. Silva, G. Nolasco, Eur. J. Plant Pathol. 133, 229–235 (2012)

    Article  CAS  Google Scholar 

  14. C. Sire, M. Bangratz-Reyser, D. Fargette, C. Brugidou, Virol J. 5, 55 (2008)

    Article  PubMed  Google Scholar 

  15. H.Y. Chen, J. Yang, C. Lin, Y.A. Yuan, EMBO J. 9, 754–760 (2008)

    Article  CAS  Google Scholar 

  16. C. Torres-Barcelo, S. Martin, J.A. Daros, S.F. Elena, Genetics 180, 1039–1049 (2008)

    Article  PubMed  CAS  Google Scholar 

  17. M.L. Yambao, H. Yagihashi, H. Sekiguchi, T. Sekiguchi, T. Sasaki, M. Sato, G. Atsumi, Y. Tacahashi, K.S. Nakahara, I. Uyeda, Arch. Virol. 153, 105–115 (2008)

    Article  PubMed  CAS  Google Scholar 

  18. J. Ye, J. Qu, J.F. Zhang, Y.F. Geng, R.X. Fang, FEBS Lett. 583, 101–106 (2009)

    Article  PubMed  CAS  Google Scholar 

  19. H.J. Maree, M.J. Freeborough, J.T. Burger, Arch. Virol. 153, 755–757 (2008)

    Article  PubMed  CAS  Google Scholar 

  20. G.P. Martelli, A.A. Agranovsky, M. Bar-Joseph, D. Boscia, T. Candresse, R.H. Coutts, V.V. Dolja, B.W. Falk, D. Gonsalves, W. Jelkmann, A.V. Karasev, A. Minafra, S. Namba, H.J. Vetten, G.C. Wisler, N. Yoshikawa, Arch. Virol. 147, 2039–2044 (2002)

    Article  PubMed  CAS  Google Scholar 

  21. P. Gouveia, M.T. Santos, J.E. Eiras-Dias, G. Nolasco, Arch. Virol. 156, 413–420 (2011)

    Article  PubMed  CAS  Google Scholar 

  22. A. Jooste, G. Pietersen, J. Burger, Eur. J. Plant Pathol. 131, 371–381 (2011)

    Article  Google Scholar 

  23. A.M. Sharma, J. Wang, S. Duffy, S. Zhang, M.K. Wong, A. Rashed, M.L. Cooper, K.M. Daane, R.P. Almeida, PLoS One 6, e26227 (2011)

    Article  PubMed  CAS  Google Scholar 

  24. P. Gouveia, S. Dandlen, A. Costa, N. Marques, G. Nolasco, Eur. J. Plant Pathol. 133, 237–245 (2012)

    Article  CAS  Google Scholar 

  25. J.C. Reed, K.D. Kasschau, A.I. Prokhnevsky, K. Gopinath, G.P. Pogue, J.C. Carrington, V.V. Dolja, Virology 306, 203–209 (2003)

    Article  PubMed  CAS  Google Scholar 

  26. J. Wang, A.M. Sharma, S. Duffy, R.P. Almeida, Phytopathology 101, 445–450 (2011)

    Article  PubMed  Google Scholar 

  27. M. Karimi, D. Inze, A. Depicker, Trends Plant Sci. 7, 193–195 (2002)

    Article  PubMed  CAS  Google Scholar 

  28. J. Haseloff, K.R. Siemering, D.C. Prasher, S. Hodge, Proc Natl Acad Sci USA 94, 2122–2127 (1997)

    Article  PubMed  CAS  Google Scholar 

  29. M.T. Ruiz, O. Voinnet, D.C. Baulcombe, Plant Cell 10, 937–946 (1998)

    PubMed  CAS  Google Scholar 

  30. R. Hofgen, L. Willmitzer, Nucleic Acids Res. 16, 9877 (1988)

    Article  PubMed  CAS  Google Scholar 

  31. M.W. Pfaffl, Nucleic Acids Res. 29, e45 (2001)

    Article  PubMed  CAS  Google Scholar 

  32. D. Rotenberg, T.S. Thompson, T.L. German, D.K. Willis, J. Virol. Methods 138, 49–59 (2006)

    Article  PubMed  CAS  Google Scholar 

  33. K. Tamura, D. Peterson, N. Peterson, G. Stecher, M. Nei, S. Kumar, Mol. Biol. Evol. 28, 2731–2739 (2011)

    Article  PubMed  CAS  Google Scholar 

  34. G. Brigneti, O. Voinnet, W.X. Li, L.H. Ji, S.W. Ding, D.C. Baulcombe, EMBO J. 17, 6739–6746 (1998)

    Article  PubMed  CAS  Google Scholar 

  35. C. Himber, P. Dunoyer, G. Moissiard, C. Ritzenthaler, O. Voinnet, EMBO J. 22, 4523–4533 (2003)

    Article  PubMed  CAS  Google Scholar 

  36. M.R. Albiach-Marti, C. Robertson, S. Gowda, S. Tatineni, B. Belliure, S.M. Garnsey, S.Y. Folimonova, P. Moreno, W.O. Dawson, Mol. Plant Pathol. 11, 55–67 (2010)

    Article  PubMed  CAS  Google Scholar 

  37. C. Torres-Barcelo, J.A. Daros, S.F. Elena, Mol. Biol. Evol. 27, 543–551 (2010)

    Article  PubMed  CAS  Google Scholar 

  38. V.A. Klaassen, S.T. Sim, G.S. Dangl, F. Osman, M.A. Rwahnih, A. Rowhani, D.A. Golino, Plant Dis. 95, 657–665 (2011)

    Article  Google Scholar 

  39. M. Wang, F. Fei, T. Zhou, Y. Cheng, Z. Fan, Acta Phytopathol. Sin. 39, 458–465 (2009)

    Google Scholar 

  40. C. Turturo, P. Saldarelli, D. Yafeng, M. Digiaro, A. Minafra, V. Savino, G.P. Martelli, J. Gen. Virol. 86, 217–224 (2005)

    Article  PubMed  CAS  Google Scholar 

  41. A.E.C. Jooste, H.J. Maree, D.U. Bellstedt, D.E. Goszczynski, G. Pietersen, J.T. Burger, Arch. Virol. 155, 1997–2006 (2010)

    Article  PubMed  CAS  Google Scholar 

  42. E.A. Engel, C. Girardi, P.F. Escobar, V. Arredondo, C. Dominguez, T. Perez-Acle, P.D.T. Valenzuela, Virus Genes 37, 110–118 (2008)

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

The authors gratefully acknowledge the assistance of S. Dandlen for help with the statistical calculations. This work was supported by the research project PTDC/AGR-GPL/099512/2008 funded by Fundação para a Ciência e Tecnologia, Portugal. Paulo Gouveia is the recipient of the PhD Student grant SFRH/BD/41826/2007.

Author information

Authors and Affiliations

  1. Center for Biodiversity, Functional and Integrative Genomics, Faculdade de Ciências e Tecnologia, Universidade do Algarve, Campus de Gambelas, 8005-139, Faro, Portugal

    Paulo Gouveia & Gustavo Nolasco

Authors
  1. Paulo Gouveia
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Gustavo Nolasco
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Gustavo Nolasco.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gouveia, P., Nolasco, G. The p19.7 RNA silencing suppressor from Grapevine leafroll-associated virus 3 shows different levels of activity across phylogenetic groups. Virus Genes 45, 333–339 (2012). https://doi.org/10.1007/s11262-012-0772-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11262-012-0772-3

Keywords

Search

Navigation