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Sequence and phylogenetic analysis of S1, S2, M, and N genes of infectious bronchitis virus isolates from Malaysia

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Abstract

Two Malaysian infectious bronchitis virus isolates, MH5365/95 and V9/04 were characterized based on sequence and phylogenetic analyses of S1, S2, M, and N genes. Nucleotide sequence alignments revealed many point mutations, short deletions, and insertions in S1 region of both IBV isolates. Phylogenetic analysis of S1 gene and sequences analysis of M gene indicated that MH5365/95 and V9/04 belong to non-Massachusetts strain. However, both isolates share only 77% identity. Analysis based on S1 gene showed that MH5365/95 shared more than 87% identity to several Chinese strains. Meanwhile, V9/04 showed only 67–77% identity to all the previously studied IBV strains included in this study suggesting it is a variant of IBV isolate that is unique to Malaysia. Phylogenetic analysis suggests, although both isolates were isolated 10 years apart from different states in Malaysia, they shared a common origin. Analysis based on S2 and N genes indicated that both strains are highly related to each other, and there are fewer mutations which occurred in the respective genes.

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Acknowledgments

The authors wish to thank the Ministry of Science, Technology and Innovation, Government of Malaysia for the funding provided under the Intensified Research in Priority Area Proposal Grant (IRPA). The authors wish to thank Dr Maizan Mohamed from Veterinary Research Institute, Ipoh, Perak for providing the MH5365/95 virus.

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Authors and Affiliations

  1. Faculty of Veterinary Medicine, University Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    Zarirah M. Zulperi, A. R. Omar & S. S. Arshad

  2. Institute of Bioscience, University Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    A. R. Omar

Authors
  1. Zarirah M. Zulperi
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  2. A. R. Omar
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  3. S. S. Arshad
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Corresponding author

Correspondence to A. R. Omar.

Additional information

The nucleotide sequence reported in this article has been submitted to EMBL/GenBank database and has been assigned the accession numbers EF591038, EF591037 for the M gene, EF591036, EF591035 for the N gene, FJ518779, FJ518780 for the S1 gene, and FJ518781, FJ518782 for the S2 gene.

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Zulperi, Z.M., Omar, A.R. & Arshad, S.S. Sequence and phylogenetic analysis of S1, S2, M, and N genes of infectious bronchitis virus isolates from Malaysia. Virus Genes 38, 383–391 (2009). https://doi.org/10.1007/s11262-009-0337-2

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  • DOI: https://doi.org/10.1007/s11262-009-0337-2

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